How does the shaker agitation rate and orbit affect culture growth?

Generally, agitation speed and orbit affect both the aeration and the mixing of the culture. Greater aeration increases oxygen transfer rate (OTR), boosting cell growth. The size of the orbit affects OTR linearly and the speed has a square-law relationship. Therefore, if the size of the orbit doubles, say from 1/2 to 1," the amount of OTR should double〖RPM〗_1/〖RPM〗_2 = (r_2^2)/(r_1^2 ), then 〖RPM〗_2 = √(〖RPM〗_1^2 (r_1/r_2 ) ). If the speed doubles, the OTR should quadruple. Of course, these are general rules and caution should be taken with shear sensitive cells. However, this information can be used to estimate the conditions needed to obtain the same results on different model shakers. For example, to get the same oxygen transfer in a shaker with a 1 in orbit running at 300 rpm, a shaker with 3/4 in orbit must be run at 350 rpm.

Do Eppendorf shakers come with platforms?

Each Eppendorf shaker is available with multiple platform options, which are ordered separately to give you the flexibility to choose the one that best suits your needs. Dedicated platforms include installed clamps of a single size (ie. only 250 mL clamps) and provide in most cases the best capacity but does not allow for the use of different size clamps or holders. Universal Platforms are designed for use with a combination of various-sized clamps and test tube racks providing maximum flexibility. When ordering a Universal Platform, clamps and test tube racks must also be ordered separately.

What is the temperature offset function?

The temperature offset function on many of our shakers allow you to match a reference point" temperature inside the chamber (culture medium) with the "Actual Value" displayed. The temperature offset function on Innova Incubated shakers allows you to monitor the temperature of the media providing you a clearer and more accurate picture. Please be aware that matching reference point temperatures to the Actual means you have to deal with many variables such as: greater evaporation; heat gains generated by cultures growing - (slowly at first and much more during their growth phase) and heat generated by the friction of media with the glass at high speeds or use of baffled flasks. Over time, the reference point temperature offset may need to change due to these varying conditions.

I want to grow my cultures at 27°C, do I need an incubated shaker with refrigeration?

The Eppendorf Incubated, non-refrigerated Shakers Innova and I-Series are designed to maintain temperatures of ambient plus 5 °C to its maximum operating temperature. The Eppendorf Incubated Excella shakers will operate from ambient plus 7 °C to maximum temperature. It is critical to understand that "ambient temperature is not the temperature on the wall thermometer (room temperature), but the temperature measured one meter from the front of the machine.

Why are some flask clamps supplied with an extra spring set?

This "extra spring set" (we call it a girdle) is included with clamps for 2 L, 2.8 L, 4 L and 6 L flasks. It is intended to be used with the already installed spring set. This girdle helps prevent large flasks from spinning inside the clamp and reduces the risk of flasks becoming dislodged or scored by the steel clamp. One girdle is already in place on the clamp, the other is packed separately. To install this extra “girdle”, follow the instructions in the manual supplied with the shaker.

How is temperature accuracy defined by Eppendorf?

Accuracy is defined as the degree of closeness between the displayed value and the actual value as determined by a traceable device. All Eppendorf Incubated Innova and I-Series shakers have a temperature Accuracy of ±0.1 °C from 30 – 40 °C, ±0.5 °C over the remaining range. New Brunswick Incubated Excella Shakers are accurate within 0.1°C at 37°C.

How is Uniformity defined by Eppendorf?

Uniformity is the ability to maintain uniform temepratures throughout the platform. New Brunswick Incubated Innova Shaker temperatures will not deviate by more than +/- 0.25 °C at 37 °C.

The orbit of a shaker is the distance traveled around the central drive. This can be descibed in inches, centimeters, or milimeters. If our shakers had a one inch (2.54 cm) orbit. That means that that the lateral movement of the platform would equal one inch from one point to another that is 180° opposite that position. All of the New Brunswick. shakers have a circular orbit

Which screws will fit to which platform ?

All of Eppendorf Inc. clamps and accessories are packaged with multiple screws to fix them to our universal platforms, included in M1013-0261 clamp screw kit (Stainless steel platform screw #S2117-3050 (10-32 x 5/16 in (7.9mm)), Stainless Steel, Aluminum, and Aluminum and Phenolic (Plastic) #S2116-3051 (10-24 x 5/16 in (7.9 mm)), and Wood #S2116-3101 (10-24 x 5/8 in (15.87mm)).

How can I disinfect, clean, maintain my shaker?

We recommend cleaning the exterior of our units with a damp cloth or any standard household or laboratory cleaner to wipe the surfaces clean. Do not use abrasive or corrosive compounds to clean the instruments as they may damage the unit and void the warranty. It is the responsibility of theuser to carry out the proper decontamination procedures if hazardous materials are spilled on or inside the equipment. Before using any cleaning or decontamination method other than those suggested by the manufacturer, users should contact Eppendorf Inc. to ensure that the proposed method will not damage the equipment. Commercially available household bleach solutions, when diluted at a 1:10 ratio, are effective in routine decontamination of the instrument. The method for decontaminating a spill depends on the nature of the spill. Spills involving fresh cultures or samples of known concentrations of biomass should be flooded with decontamination solution and soaked for at least 5 minutes before cleanup. Spills involving samples with high concentrations of biomass, or involving organic matter, or occuring in areeas warmer than ambient/room temperature, should be exposed to decontamination solution for at least one hour before being cleaned up. Personnel involved in the cleanup of any spill should wear gloves, safety glasses, and a laboratory coat or gown during the cleanup process. Respiratory protection should be considered for spills where aerosolization is suspected.

Which flask type fits in which clamp ?

Please see the Accessories for New Brunswick™ Shakers: Dimensions and Capacities in the Userguides section of the shaker product page.

Which vessel fits in Eppendorf Test tube racks ?

Please see the Accessories for New Brunswick™ Shakers: Dimensions and Capacities in the Userguides section of the shaker product page.

Why is the triple eccentric drive special ? What is the difference between Innova and Excella Shakers drive ?

The Innova and I-series Triple eccentric drive consists of three individual bearings and eccentric shafts mounted in a triangular fashion for precise uniform circular motion at every point on the platform. The heavy duty drive has a single shaft that utilizes springs to aid in balancing the load. This drive works great with small to medium loads at lower speeds.

How can I shake MTPs ? Which parameters ?

Eppendorf shakers offer a variety of Micro Titer Plate holders that attach to our univeral platforms. Our shakers offer a variety of orbits up to 5.1 cm (2 in) and speeds up to 500 rpm. If mixing is what is needed at higher speeds and smaller orbits you can find Eppendorf Mixers.

How do I use of sticky pads/sticky tape?

Eppendorf Sticky Pads are recommended for use on our sticky pad platform or our sticky pad adapter kit (M1250-9504). On the S44i universal platform sticky pad can be used directly. The adapter kit is easily fixed to our universal platforms. Any size flask can be used with the sticky pad with a 1 in (2.54 cm) orbit up to 250 rpm, any size flask can be used with the 2 in (5.1 cm) orbit up to 200 rpm. To clean the sticky pad, just wash with soap and water. this will revive" the stickiness, but the sticky pad must be completely dry before replacing on the platform.

What is the heavy load platform ?

The Heavy Duty Platform (M1282-9928) is designed for use in the Innova 44 only. This platform is 0.95 cm thick alluminum plate that is recommended for shaking full loads of flasks greater than 1 L at or above 20 % fill volumes or flasks with baffles.

What is the orbit of a shaker?

An orbital shaker moves the platform in a circular shaking motion. The term orbit describes the diameter of this circular movement. Typical orbit sizes are 1.9 cm, 2.5 and 5 cm.

What orbit shall I take?

An orbital shaker is used to grow microorganisms or cells growing in suspension, like bacteria, yeast, fungal, insect, algae or plant cells. Orbital shaking enables a homogeneous distribution and aeration of the cultures by increasing the oxygen transfer rate (OTR). There are different influencing parameters on shaking performance, e.g. the shaker orbit, the applied speed, the vessel shape, the liquid viscosity and the filling volume. The 2.5 mm orbit is the standard solution which works well for most applications. For some applications, e.g. large vessels > 2 Liter, especially large volume Fernbach flasks or very shear sensitive cells, a larger shaking diameter with the consequence of a reduced speed can be beneficial.

I use two shakers with different orbits. Can I transfer my experimental conditions to a shaker with a different orbit?

Yes, the rpm can be adapted with help of the following formula .

What platform types are offered with the shakers?

Universal platform and a variety of dedicated platforms are available. University platforms offer maximum flexibility having a hole-pattern that allows using virtually any combination of clamps and other accessories. Dedicated platforms come complete with pre-installed clamps of one size. The hole-pattern of the dedicated platform is optimized for the use of one specific size of clamps. It allows in most cased more capacity but can only be used with that size of clamp. Please note that the platform has to be ordered in addition to the shaker.

Why should I have my incubator serviced at regular intervals?

Eppendorf Performance Plans offer a selection of preventive maintenance services which ensure a consistent performance of the device and confidence in the results.

How often does my incubator need to be serviced?

This depends on how intensively it is used and varies depending on the load on the device. However, we recommend to have it serviced at least once a year.

What do you need to look out for before carrying out a service?

The device needs to be decontaminated first. The device must be freely accessible and all samples removed from the device. You should also ensure that the device has cooled down already.

What types of service programs are available for incubators?

各器機に提供されているサービスプログラムは、サービス＆サポート/メンテナンスおよび証明書サービスセクションにあります。

How much are the different incubator service programs?

The cost depends on the model and on the service that is performed. Please contact your local Eppendorf Service. www.eppendorf.com/contact

Please contact your local Eppendorf Service Team. You find the contact data on our homepage or in the information materials. www.eppendorf.com/contact

What is IQ or OQ and what do I need them for?

Installation and operational qualification; the IQ documents that the device has been delivered and installed correctly. The OQ checks the correct functioning of a device. IQ/OQ ensure that your quality management requirements are met and that your device operates properly and in accordance with the manufacturer’s specifications.

How often does the OQ need to be carried out for an incubator?

The company is responsible for technical checks of devices. Eppendorf recommends regular device qualifications depending on the intensity of use, however, they should be carried out at least once a year.

How can my incubator be cleaned/ disinfected?

Use a solution of 70% isopropanol (isopropyl alcohol) and 30% distilled water as a disinfectant. Follow the cleaning guidelines carefully that are described in your user manual.

Which parts of the incubator should be replaced at regular intervals?

Replace the HEPA filter of the CO 2 supply line and the HEPA filter with automatic zero setting * at least once a year. (* Auto-zero filter is only used in R-models.)

What types of training or online training do you offer to enable me to improve my skills?

Please visit the Eppendorf webpage about the Eppendorf Training Center or contact your local Eppendorf Service for further information and a quotation. www.eppendorf.com/training

Where are the sensors, the access port (for cables) and the gas sample port located in the S41i? Is there an overview of the inner chamber parts (in terms of sensors and in/outlets)?

The S41i has two temperature sensors (one on the top ceiling, one in the back wall below the platform. It has one access port in the back wall to enter cables e.g. from external sensors. It has in the back one inlet for CO2 gas and one outlet for gas sampling. Please see attached document for picture.

Why should I have my Orbital Shaker serviced at regular intervals?

As with all products with moving parts, wear and tear is inevitable and can affect how consistently the product operates over a long period of time. Eppendorf products are engineered to minimize wear and tear, but a periodic service allows early identification and tracking of excessive wear before product performance is outside of acceptable tolerances. Regular servicing may also be a regulatory requirement of our users.

How often does my orbital shaker need to be serviced?

Eppendorf recommends a service once every 12 months. However, the regulatory requirements of our users vary greatly and can often be more frequent.

How much are the different orbital shaker service programs?

The cost depends on the product variant and on the level of service that is required. Please contact your local Eppendorf Service centre for a personal quotation. www.eppendorf.com/contact

What is IQ or OQ and what do I need them for?

Installation and Operational Qualification; the Installation Qualification documents that the product has been delivered and installed in accordance with manufacturer recommendations and guidelines. The Operational Qualification checks the correct functioning of a device in accordance with manufacturer recommendations and guidelines (as such, it can only be provided by the equipment manufacturer). IQ/OQ is primarily required in organizations that implement quality management processes but is available to all users.

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New Brunswick S41i - CO₂インキュベーターシェーカー

New Brunswick™ S41i CO2 Incubator Shaker

New Brunswick™ S41i CO2 Incubator Shaker Double-Stack Configuration

製品情報

高温消毒を内蔵し、大幅にガス消費を減らすCO₂ インキュベーターシエーカーを探していますか？クリーニングが簡単になり、CO₂ シリンダーの交換頻度を軽減します。ファンなしで、スタック可能な New Brunswick S41i付き。

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New Brunswick S41i

170 L, CO₂インキュベーターシェーカー、内部シェルフとタッチ画面コントロールを装備
最大2重に積み重ね可能, 1 (2. optional) コンパートメント, 120 V ± 10 %/50/60 Hz (US), 軌道 2.5 cm（1 インチ）

製品のさらなる情報

数量

在庫確認してください

カタログ番号 S41I120010

希望小売価格

¥ 3,220,000

税込価格

¥ 3,542,000

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製品情報

テクニカルデータ

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特徴

結果の再現性（タンパク質発現など）は、大気の精度とファンによる培地蒸発からの保護に大きく依存していますか？シェーカーの汚染によるサンプルの損失のリスクはありませんか？そして、プログラミング、CO₂シリンダーの交換、または信頼性の高いクリーニングに時間をかけたくありませんか？

S41i CO₂インキュベーターシェーカーが提供するスマートで汎用性の高いソリューションをご覧になり、現在のソリューションと比較してください。
• ビルトインNew Brunswickシェーカー付きCO₂インキュベーター
• 高温消毒（120 °Cで4 時間）標準
• リアルタイムおよび過去の状態の広範囲で迅速な分析
• マルチパラメータのクイックビューイング用の大きな読みやすいディスプレイ、補助スクリーン、トレンドグラフィックそして診断
• 直観的なコントローラなので環境とアラームセッティングを迅速に変更でき、スクリーン上でトラブルシューティングと診断が可能。
• 72時間継続データロッギングと温度、アラーム、ドア開閉そして環境状態の詳細記録
• パスワードで保護されたセッティングとアラームセットポイント

VisioNize^® Lab Suite

を使って効率的なラボと装置管理ラボ装置の監視や通知の受信（装置アラームのためなど）に関心がありますか？デジタル接続されたラボは、さらに多くの事を提供します。

モジュール式バンドルソフトウェアサービスにアクセスし、ラボに適したものを選択します。VisioNize Lab Suiteは、お客様がラボ管理者や科学者として、試料のセキュリティ、コンプライアンスのニーズ、メンテナンス管理のためのラボのデジタル管理を開始するために必要なものすべてを提供します。

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仕様	New Brunswick S41i	New Brunswick S41i

外部の深さ（蓋/ドアが開いている状態）	144.8cm（57インチ）	144.8cm（57インチ）
容量	170 L (6.0 ft³)	170 L (6.0 ft³)
シェルフの数	1 (2. optional)	1 (2. optional)
タイマー	連続	連続
電源	100 V ± 10 %, 50/60 Hz	120 V ± 10 %, 50/60 Hz
寸法（W × D × H）	87.5 × 73 × 85 cm / 34.4 × 28.7 × 33.5 in	87.5 × 73 × 85 cm / 34.4 × 28.7 × 33.5 in
製品の重量	154 kg / 340 lb	154 kg / 340 lb
高温消毒（HTD）	120 °C	120 °C
シールされた内部ガラスドア	はい	標準ヒョウジュン
セグメントドアオプション	–	–
温度制御インクリメント	±0.1 °C	±0.1 °C
温度安定性 @ 37 °C	±0.1 °C	±0.1 °C
温度範囲	周囲温度+4 ℃～50 ℃	周囲温度+4 ℃～50 ℃
可聴アラームと視覚アラーム	はい	はい
温度の均一性	37 °Cで±0.25 °C（フラスコ内の培地）	37 °Cで±0.25 °C（フラスコ内の培地）
CO₂ 範囲	0.2 – 20 %	0.2 – 20 %
利用可能なプログラムモード	定速と定温	定速と定温
CO₂ 制御増分	±0.1 %	±0.1 %
CO₂ 安定性 @ 5 % CO₂	±0.2 %	±0.2 %
ドライブ機構	トリプル偏心カウンターバランスドライブ	トリプル偏心カウンターバランスドライブ
CO₂ 均一性	±0.1 %	±0.1 %
シェルフの数 (標準/最大)	1/2	1/2
データエクスポートインターフェース	Ethernet	Ethernet
ベンチトップ(B)、アンダーベンチ(U、フロア(F) 、スタック式 (S)	U, F, S (x2)	U, F, S (x2)
アクセスポート	1	1
オンボードのデータログ	data display (no data log or export)	標準ヒョウジュン
BMSリレー	はい	標準ヒョウジュン
メモリー	停電時の自動再起動による不揮発性	停電時の自動再起動による不揮発性
モータータイプ	ソリッドステート、DCブラシレスモーター	ソリッドステート、DCブラシレスモーター
プラットフォームサイズ	36 × 61 cm（14 × 24 インチ）	36 × 61 cm（14 × 24 インチ）
ドアオープニング	前開き	前開き
速度範囲	25 – 400 rpm （積み重ねられている：25 - 250/400 rpm トップ/ボトムユニット用）	25 – 400 rpm （積み重ねられている：25 - 250/400 rpm トップ/ボトムユニット用）
cETLusとCEマーク付き	はい	はい
容量	中	中
穿刺シェルフ	−	標準ヒョウジュン
リモート装置監視および通知 (VisioNize^®)	VisioNize^®ボックス可能	VisioNize^®ボックス可能
シェーキング	−	標準
温度範囲	周囲温度+4 °C～50 °C	周囲温度+4 °C～50 °C
温度精度	±0.2 °C	±0.2 °C
シームレスチャンバー	−	標準ヒョウジュン
インラインガスレギュレーター	−	標準ヒョウジュン

撹拌
軌道	2.5 cm（1 インチ）	2.5 cm（1 インチ）