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          Lab scientist with Eppendorf BioSpectrometer® basic photometer in the foreground.

          測光

          製品ラインアップ

          Mechanism of Eppendorf µCuvette G1.0

          キュベット

          BioPhotometer Reference filter set

          プリンターやフィルターセット

          laboratory software, VisioNize

          Sample and Information Management

          1950年、Eppendorfは最初の分光測定器を市場に送り出しました。現在の製品ポートフォリオには分光測定の分野で培ってきたEppendorfのノウハウが結集されており、必要な装置とアクセサリーをバリエーション豊かなラインナップからお選びいただけます。
          Eppendorf Detectionの製品ラインナップは、核酸/タンパク質の定量、酵素反応、ELIZA、蛍光分析など、分子レベルの分析を簡単、効率的に行う性能を提供します。µCuvette G1.0を備えたキュベットでの微量測定も可能です。

          When do I need what?

          Depending on the sample concentration,
          different photometric approaches are
          recommended:

          when_do_i_need_what
          Eppendorf picture

          Extinction

          • Quick and easy direct measurement method Harmless and stable, since no chemicals have to be used Provides information about sample purity
          • Harmless and stable, since no chemicals have to be used
          • Provides information about sample purity

          UV-Vis Samples

          Quantification of nucleic acids can be performed by measuring absorbance at a wavelength of 260 nm in a UV-Vis(spectro-)photometer. The OD260 value is used to calculate the concentration, using the sample specific factor, of the nucleic acid sample.

          OD260 = 1* : 50 μg/ mL double strand DNA
          40 μg/ mL RNA
          33 μg/ mL single strand DNA

          uv_vis_samples_final
          flourescence_samples_new

          Fluorescence Samples

          UV-based quantification of nucleic acids has limits which can be bypassed by using fluorescence detection, qPCR, or chip-based capillary electrophoresis systems.

          The amount of nucleic acid is calculated based on the intensity of the fluorescent signal. For excitation and detection, fluorimeter or a photometer with a fluorescence module is required.

          Nucleic acids quantification via fluorescence is based on fluorescent dyes which bind to the nucleic acids. Only the complex consisting of nucleic acid and dye is excited by light of a specific wavelength (dependent on the dye) and will subsequently emit light of a slightly longer wavelength.

          Annoyed by uncomfortable handling?

          Ergonomics is far beyond the »ergonomically designed chair«. Eppendorf started to optimize the laboratory devices regarding ergonomics already in the early 1970s.In 2003, we started the PhysioCare Concept, focusing on ergonomic liquid handling devices like our pipettes. Nowadays, the Eppendorf PhysioCare Concept is broaden up to include further laboratory products as a holistic solution to harmonize the workflow in your laboratory with your health and well-being, e.g. for the Eppendorf BioSpectrometer:

          • Guided software for easy handling
          • Clear indications at cuvette shaft for convenient handling
          • Direct keys for convenient handling
          • Small footprint to enable position in direct arm reach at your bench
          • Standardized »user-interface concept« for intuitive handling of devices
          Further information about the Eppendorf PhysioCare Concept:

          www.eppendorf.com/physiocare
          physiocare_logo_2

          Struggling with paper-based documentation?

          The eLABJournal Electronic Lab Notebook offers an intuitive and flexible solution to document research and track research data like BioSpectrometer–based values. Improve efficiency when documenting, organizing, searching, and archiving collected data. With the free eLABJournal add-ons, you can extend the functionality of eLABJournal to a fully integrated Laboratory Information Management System.

          Start a free 30-day trial!

          Visit
          www.eLABJournal.com

          electronic_lab_notebook
          sh_header_1953_customer_service_historical

          One of the First Photometers for Labs

          You might got your basic training in the laboratory with one of ours… Based on nearly 70 years of photometry experience, Eppendorf has developed many different photometers over the time.

          Eppendorf Japan

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