Are Eppendorf products free of RNA?

All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.

What is the meaning of the PCR Clean seal?

For molecular-biological requirements Eppendorf offers a new standard for uniform purity specifications. All consumables marked with the "Certified PCR Clean” label are guaranteed free of human DNA, DNase, RNase and PCR inhibitors.

Why are there double labels on the folding boxes, as "PCR clean" + "Sterile" or "PCR clean" + "Protein-free"?

Due to the modularization of purity grades, some products are labeled with more than one purity grade symbol. For example, the epDualfilter T.I.P.S. has the double seal "PCR clean "+ "Sterile" and the UVette the double seal "PCR clean" + "Protein-free" on the packing. The certification remains unaffected by the modularization.

Are lot specific certificates for the purity grades available in the internet?

Yes, the lot specific purity certificates, so-called Test report, can be downloaded at the certificates homepage .

Can products of the previous and new modular purity grades (with new seals) be used simultaneously in one application?

Yes, if no batch attachment is required for the application, products of the old and new modular purity grades can be used, as their specifications are the same.

Which advantages do Eppendorf Polypropylene Microplates have over plates made from polystyrene?

Plates made from polypropylene may be used across a broad spectrum of applications, as they are more resistant to temperature variations as well as various chemicals than plates made from polystyrene. Furthermore, they are more robust, allowing them to be centrifuged at higher g-forces. In addition, biomolecules, such as nucleic acids and proteins, bind less strongly to polypropylene.

Are Eppendorf PP Microplates resistant to organic solvents, e.g. DMSO?

The Eppendorf PP Microplates are made from polypropylene and are thus resistant to DMSO and other organic solvents, unlike plates made from polystyrene. An overview of chemical resistance can be found in Application Note no. 56.

Are the colored rim and labeling resistant to organic solvents?

All areas of the plate, including the colored rim, are resistant to chemicals. The alpha numeric labeling is equally durable. An overview of chemical resistance can be found in Application Note no. 56.

How may the Eppendorf PP Microplates be sealed?

Eppendorf offers a variety of sealing options for Microplates. Self-adhesive sealers, films and foils for heat sealing, as well as re-usable sealing mats, are available. Information regarding the compatibility of the different sealing methods is listed in the Application Note no. 208.

Which advantages do the black Eppendorf PP Microplates offer?

Plates made from black material are especially well suited for fluorescence assays (e.g. PicoGreen ® ). The black wells prevent “cross talk” between wells and reduce background signals. The black Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in black plates. Due to carefully selected materials, the plates have very low autofluorescence. The superior signal-to-noise ratio contributes to the increased sensitivity of fluorescence assays (see Application Note no. 203).

Which advantages do the white Eppendorf PP Microplates offer?

Plates made from white material may be used for luminescence assays (chemiluminescence, scintillation proximity assays (SPA) etc.), as well as for fluorescence assays (fluorescence intensity, polarization, etc.). The white wells prevent “cross talk” between the wells while amplifying the individual signals. The white Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in white plates. Due to carefully selected materials, the plates offer maximum signal amplification. Due to their very low autofluorescence, the white Eppendorf Microplates are equally well suited for fluorescence assays. Taken together, these features allow for an improvement in the sensitivity of analyses.

May the Eppendorf PP Microplates be stored at –196°C in liquid nitrogen?

No, we recommend storage at a minimum of –80°C.

May the Eppendorf PP Microplates be autoclaved?

Yes; however, the exact specifications of the Microplates could be compromised. The plates are available in the qualities sterile or PCR clean. Hence, autoclaving is necessary only under exceptional circumstances.

Are the Eppendorf PP Microplates in LoBind quality coated or siliconized?

No, the LoBind quality of the Eppendorf PP Microplates is not derived from siliconization or any other type of coating. The functionality is achieved through a carefully selected material, in combination with a special manufacturing process.

How high may Eppendorf PP Microplates be centrifuged?

The Eppendorf PP Microplates may be centrifuged up to 6000 x g. Please follow the instructions provided in the manual.

May immunoassays such as ELISA be performed in the Eppendorf PP Microplates?

Plates made from polypropylene are not suited for the standard variant, where antigen or antibodies are bound to the well wall. For this application, plates made from polystyrene are used, which have a higher binding capacity for biomolecules. However, for antibody-based fluorescence or luminescence assays (e.g. with beads) Eppendorf PP Microplates with black or white wells are suitable, respectively.

Is it possible to perform absorption measurements in the Eppendorf PP Microplates?

Typically, absorption measurements are not performed in plates made from polypropylene, but in plates made from polystyrene, as this material generally has a very high transparency. Since the Eppendorf PP Microplates show considerably higher transparency compared to other plates made from polypropylene (see Application Note no. 202), the Eppendorf Microplates may be used for absorption measurements in certain cases.

Why are the Eppendorf PP Microplates offered in Sterile and PCR clean, but not in Eppendorf quality?

The Eppendorf PP Microplates are a very high quality product. In order to underline the quality, all plates are available in the degrees of purity PCR clean or Sterile. This increases the confidence and comfort of the user, who may employ the plates directly for valuable samples in sophisticated applications.

Are Eppendorf PP Microplates also available with barcode?

Yes, the option of Eppendorf Microplates with barcode is available. Further information can be found on the product page microplates .

Are Eppendorf tubes and plates (also LoBind variants) resistant to 100% acetonitrile?

Eppendorf tubes and plates including LoBind variants are resistant against 100% acetonitrile only in room temperature conditions. Above 40°C and prolonged exposure (days) may lead to partial damage and is not recommended. For lower acetonitrile concentrations the resistance parameters will be better so prolonged exposure and higher temperatures may be possible. This must be however tested by the customer.

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Eppendorf Microplates

Eppendorf Microplate with OptiTrack® matrix

Selection of microplates stacked on top of each other

Four microplates, 384-well black and transparent plate, 96-well white and transparent plate

Sample being pipetted into a microplate with Combitip® pipette tips

Eppendorf Microplate in an automated liquid handling instrument

Woman in lab coat looking through microplate

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Eppendorf Microplatesで新しいレベルの透明性を体験してください！最高のクオリティのポリプロピレンと優れたウェルデザインで、最小の手間でピペッティング、管理、回収が可能です。さらに、弊社はどのアプリケーションにも対応するプレートを提供します。

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簡単に使えるEppendorf Microplatesで試料を管理し回収してください！読み取りやすい英数字 OptiTrack^®マトリックスラベル付きの設計なので個々のウェルを識別でき充填処理をスピードアップし、ピペッティングエラーのリスクを軽減します。Eppendorf Microplatesは高い透明性のポリプロピレンで出来ており、試料の場所を迅速にそして簡単に確認することができ、最大の試料回収が必要な時のためにEppendorf LoBind^®にあります。

優れた透明性とOptiTrack^®マトリックス

弊社のEppendorf Microplatesは最高のクオリティのポリプロピレンで製造されており、最大の透明性が得られ、再現性を改善するので安心です。コントラスト豊富なOptiTrackマトリックスでラボ効率を上げましょう – 記録的なタイムでウェルを識別し、エラーと汚染のリスクを軽減します。
アプリケーションノート202を読んで Eppendorf Microplatesが素早く試料を識別する方法を学習してください：Eppendorfポリプロピレンマイクロプレート – 試料を迅速かつ確実に識別できます。

RecoverMax^®ウェル設計で最小の時間で最大の回収

Eppendorf最大の試料回収とマイクロプレートのRecoverMax^®最適化されたウェル形状で優れたミキシング特性を掴んでください。ウェルコーナーの丸型でスムーズな設計が試料を損失から保護し、残余量を最小化し、四角いウェルプレートの滑らかな輪郭は交差汚染をほとんど根絶します。

Eppendorf Microplatesは究極的な透明性とクオリティをさまざまなフォーマットで提供します：

• 96および384ウェルフォーマット
• Fボトム（フラット）、Uボトム（丸型）、Vボトム（コニカル）。
• Eppendorf PCR clean そしてSterile 純度等級。
• DNAおよび Protein LoBindとしても揃っています。
• g–Safe^®：最大6,000 x gという優れた遠心分離の安定性により、高速なプロトコルおよび試料品質の改善を実現。
• 縁が高くなっており、表面が滑らかなので信頼できるクロージング、ヒートシーリングも付いています
• 耐薬品性、機械的耐久性、温度安定性が高いポリプロピレン製です。
• オートクレーブ可能（121 °C、20 分)。
• スリップ剤、可塑剤、殺生物剤などの試料に影響を及ぼす恐れのある物質は用いておりません

詳細については、アプリケーションノート56を参照してください：純正Eppendorf Tubes^®とプレート用に最高級の素材：ポリプロピレンの特性と薬品耐性。アプリケーションノート239：Eppendorf Deepwell Platesおよびマイクロプレート - シーリング方法の研究。

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Quality – Stability – Purity: Eppendorf Plates^®

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Eppendorf Deepwell Plates

ハイクオリティ、優れたパフォーマンスEppendorf Deepwell Plates.

Twin.tec PCR plates offer ideal features for reproducible PCR

Eppendorf twin.tec^® PCR Plates LoBind^®

特別な製造技術により、 twin.tecPCRプレートの高品質とメリットを低結合と組み合わせて、ターゲット分子の収量を最大化します。自動化は便利です。異なるフォーマットを利用できます。

Evenly shaped PCR plates for reliable heat transfer

Eppendorf twin.tec^® PCR Plates

ツインテクノロジーの高品質PCRプレート。剛性の高いPCフレームを備えた薄壁PPウェルは、反ることなく最適な熱伝達を可能にし、非常に自動化されています。異なるフォーマットを利用できます。

An opened Eppendorf PCR tube that is certified free of a range of common PCR contaminants

Eppendorf PCR Tubes

高品質のシングルチューブとチューブストリップ。これらのチューブは最適化された熱伝達を可能にし、簡単に開けることができ、PCR内部での気化を防止するために気密なシールを提供します。異なるフォーマットを利用できます。

試料調製と貯蔵用シーリングオプション

Microplateで安全なシールオプション

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Eppendorf Microplates

Eppendorf Microplate with OptiTrack® matrix

Selection of microplates stacked on top of each other

Microplates stacked in a A-2 MTP

384-well Eppendorf Microplate

96-well Eppendorf Microplate

96-well microplates, stacked

Four microplates, 384-well black and transparent plate, 96-well white and transparent plate

Microplate in Eppendorf MixMate®

Sample being pipetted into a microplate with Combitip® pipette tips

Eppendorf Microplate in an automated liquid handling instrument

Woman in lab coat looking through microplate

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優れた透明性とOptiTrack®マトリックス

RecoverMax®ウェル設計で最小の時間で最大の回収

Eppendorf Microplatesは究極的な透明性とクオリティをさまざまなフォーマットで提供します：

アプリケーション

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Eppendorf Deepwell Plates

Eppendorf twin.tec® PCR Plates LoBind®

Eppendorf twin.tec® PCR Plates

Eppendorf PCR Tubes

試料調製と貯蔵用シーリングオプション

優れた透明性とOptiTrack^®マトリックス

RecoverMax^®ウェル設計で最小の時間で最大の回収

Eppendorf twin.tec^® PCR Plates LoBind^®

Eppendorf twin.tec^® PCR Plates