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Fibra-Cel® Disks

Fibra-Cel disks blue version, 2

製品情報

Fibra-Cel Disks are a solid support growth matrix for mammalian and insect cells, used predominantly for production of secreted products such as recombinant proteins and viruses. When combined with the Eppendorf proprietary packed-bed impeller system, Fibra-Cel Disks enable growth of adherent cells in stirred-tank bioreactors and eliminate the need for cell filtration to separate cells from the end product. Fibra-Cel Disks are suitable for the use in manufacturing environments.
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製品 (3)

    Fibra-Cel® Disks
    • 1 kg
    製品のさらなる情報
    カタログ番号 M1292-9974
      Fibra-Cel® Disks
      • 50 g
      製品のさらなる情報
      カタログ番号 M1292-9984
        Fibra-Cel® Disks
        • 250 g
        製品のさらなる情報
        カタログ番号 M1292-9988

        製品情報

        アプリケーション

        特徴

        Fibra-Cel Disks are usable in glass, single-use, and sterilize -in-place bioreactors. By combining Fibra-Cel Disks with the Eppendorf proprietary packed-bed impeller system, you create a three-dimensional matrix. The packed-bed impeller forms a compartment inside the bioreactor, in which the Fibra-Cel Disks and the cells attached to them are entrapped.
        • Proven culture success with a variety of cell lines.
        • High surface to volume ratio supports high cell densities and productivities.
        • Low-shear environment facilitates the cultivation of sensitive cells.

        Fibra-Cel Disk structure


        Fibra-Cel Disks consist of a three-dimensional polyester/polypropylene mesh to which cells attach.
        • Attachment matrix with a diameter of 6 mm.
        • Electrostatically pretreated to support cell adhesion.
        • High surface to volume ratio.
        • Provide a low-shear environment as the cells become embedded in the three-dimensional fiber system.

        Packed-bed impeller for the cultivation of adherent cells in bioreactors


        The packed-bed impeller forms a compartment inside the bioreactor, in which the Fibra-Cel Disks and the cells attached to them are entrapped.
        • High and uniform mass transfer of nutrients and oxygen.
        • Bubble-free aeration in and around the packed bed, which is advantageous for the cultivation of shear sensitive cells.
        • Low pressure drop across the bed minimizes variability and maintains viability of cells over the entire bed.

        Simple procedures from inoculation to harvest


        When you inoculate your bioreactor, the cells become entrapped within the Fibra-Cel Disk bed, where they remain throughout the process.
        • Cell attachment takes usually only up to one hour.
        • Easy harvest of medium and secreted products without the need for cell separation.
        • Easy setup of perfusion processes. The cells remain in the packed-bed which makes filtration unnecessary and therefore avoids the danger of filter clogging.

        For the use in manufacturing environments


        • Fibra-Cel Disks come with enhanced, consistent and comprehensive documentation supporting the use in manufacturing environments.
        • All polymeric materials of Fibra-Cel Disks as well as the manufacturing process can be declared as animal derived ingredient free or meet the guidelines outlined in EMA/410/01. As such, these materials can be declared as free of BSE/TSE.
        • Detailed information is available in a validation guide with the documentation to assist with the qualification of Fibra-Cel Disks in the intended process.

        Fibra-Cel Disks – Documentation package


        Customers purchasing Fibra-Cel Disks can download a turnover package with documentation, which they can use for qualification of Fibra-Cel Disks for their intended process. It contains:
        • Certificate of Analysis
        • Certificate of Quality
        • Validation Guide
        • Extractables Testing Report

        HEK 293 cells grown on Fibra-Cel Disk at day 7 of the growth cycle

        ダウンロード: Fibra-Cel® Disks

        内容

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        言語 (1)
        • 英語
        ブロシュア
        Support Growth
        Fibra-Cel® Disks – A solid support growth material for cell culture
        PDF 4.1MB
        英語

        同時にご考慮下さい。

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        Eppendorf Japan

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