How does the shaker agitation rate and orbit affect culture growth?

Generally, agitation speed and orbit affect both the aeration and the mixing of the culture. Greater aeration increases oxygen transfer rate (OTR), boosting cell growth. The size of the orbit affects OTR linearly and the speed has a square-law relationship. Therefore, if the size of the orbit doubles, say from 1/2 to 1," the amount of OTR should double〖RPM〗_1/〖RPM〗_2 = (r_2^2)/(r_1^2 ), then 〖RPM〗_2 = √(〖RPM〗_1^2 (r_1/r_2 ) ). If the speed doubles, the OTR should quadruple. Of course, these are general rules and caution should be taken with shear sensitive cells. However, this information can be used to estimate the conditions needed to obtain the same results on different model shakers. For example, to get the same oxygen transfer in a shaker with a 1 in orbit running at 300 rpm, a shaker with 3/4 in orbit must be run at 350 rpm.

Do Eppendorf shakers come with platforms?

Each Eppendorf shaker is available with multiple platform options, which are ordered separately to give you the flexibility to choose the one that best suits your needs. Dedicated platforms include installed clamps of a single size (ie. only 250 mL clamps) and provide in most cases the best capacity but does not allow for the use of different size clamps or holders. Universal Platforms are designed for use with a combination of various-sized clamps and test tube racks providing maximum flexibility. When ordering a Universal Platform, clamps and test tube racks must also be ordered separately.

What is the temperature offset function?

The temperature offset function on many of our shakers allow you to match a reference point" temperature inside the chamber (culture medium) with the "Actual Value" displayed. The temperature offset function on Innova Incubated shakers allows you to monitor the temperature of the media providing you a clearer and more accurate picture. Please be aware that matching reference point temperatures to the Actual means you have to deal with many variables such as: greater evaporation; heat gains generated by cultures growing - (slowly at first and much more during their growth phase) and heat generated by the friction of media with the glass at high speeds or use of baffled flasks. Over time, the reference point temperature offset may need to change due to these varying conditions.

I want to grow my cultures at 27°C, do I need an incubated shaker with refrigeration?

The Eppendorf Incubated, non-refrigerated Shakers Innova and I-Series are designed to maintain temperatures of ambient plus 5 °C to its maximum operating temperature. The Eppendorf Incubated Excella shakers will operate from ambient plus 7 °C to maximum temperature. It is critical to understand that "ambient temperature is not the temperature on the wall thermometer (room temperature), but the temperature measured one meter from the front of the machine.

Why are some flask clamps supplied with an extra spring set?

This "extra spring set" (we call it a girdle) is included with clamps for 2 L, 2.8 L, 4 L and 6 L flasks. It is intended to be used with the already installed spring set. This girdle helps prevent large flasks from spinning inside the clamp and reduces the risk of flasks becoming dislodged or scored by the steel clamp. One girdle is already in place on the clamp, the other is packed separately. To install this extra “girdle”, follow the instructions in the manual supplied with the shaker.

How is temperature accuracy defined by Eppendorf?

Accuracy is defined as the degree of closeness between the displayed value and the actual value as determined by a traceable device. All Eppendorf Incubated Innova and I-Series shakers have a temperature Accuracy of ±0.1 °C from 30 – 40 °C, ±0.5 °C over the remaining range. New Brunswick Incubated Excella Shakers are accurate within 0.1°C at 37°C.

How is Uniformity defined by Eppendorf?

Uniformity is the ability to maintain uniform temepratures throughout the platform. New Brunswick Incubated Innova Shaker temperatures will not deviate by more than +/- 0.25 °C at 37 °C.

The orbit of a shaker is the distance traveled around the central drive. This can be descibed in inches, centimeters, or milimeters. If our shakers had a one inch (2.54 cm) orbit. That means that that the lateral movement of the platform would equal one inch from one point to another that is 180° opposite that position. All of the New Brunswick. shakers have a circular orbit

Which screws will fit to which platform ?

All of Eppendorf Inc. clamps and accessories are packaged with multiple screws to fix them to our universal platforms, included in M1013-0261 clamp screw kit (Stainless steel platform screw #S2117-3050 (10-32 x 5/16 in (7.9mm)), Stainless Steel, Aluminum, and Aluminum and Phenolic (Plastic) #S2116-3051 (10-24 x 5/16 in (7.9 mm)), and Wood #S2116-3101 (10-24 x 5/8 in (15.87mm)).

How can I disinfect, clean, maintain my shaker?

We recommend cleaning the exterior of our units with a damp cloth or any standard household or laboratory cleaner to wipe the surfaces clean. Do not use abrasive or corrosive compounds to clean the instruments as they may damage the unit and void the warranty. It is the responsibility of theuser to carry out the proper decontamination procedures if hazardous materials are spilled on or inside the equipment. Before using any cleaning or decontamination method other than those suggested by the manufacturer, users should contact Eppendorf Inc. to ensure that the proposed method will not damage the equipment. Commercially available household bleach solutions, when diluted at a 1:10 ratio, are effective in routine decontamination of the instrument. The method for decontaminating a spill depends on the nature of the spill. Spills involving fresh cultures or samples of known concentrations of biomass should be flooded with decontamination solution and soaked for at least 5 minutes before cleanup. Spills involving samples with high concentrations of biomass, or involving organic matter, or occuring in areeas warmer than ambient/room temperature, should be exposed to decontamination solution for at least one hour before being cleaned up. Personnel involved in the cleanup of any spill should wear gloves, safety glasses, and a laboratory coat or gown during the cleanup process. Respiratory protection should be considered for spills where aerosolization is suspected.

Which flask type fits in which clamp ?

Please see the Accessories for New Brunswick™ Shakers: Dimensions and Capacities in the Userguides section of the shaker product page.

Which vessel fits in Eppendorf Test tube racks ?

Please see the Accessories for New Brunswick™ Shakers: Dimensions and Capacities in the Userguides section of the shaker product page.

Why is the triple eccentric drive special ? What is the difference between Innova and Excella Shakers drive ?

The Innova and I-series Triple eccentric drive consists of three individual bearings and eccentric shafts mounted in a triangular fashion for precise uniform circular motion at every point on the platform. The heavy duty drive has a single shaft that utilizes springs to aid in balancing the load. This drive works great with small to medium loads at lower speeds.

How can I shake MTPs ? Which parameters ?

Eppendorf shakers offer a variety of Micro Titer Plate holders that attach to our univeral platforms. Our shakers offer a variety of orbits up to 5.1 cm (2 in) and speeds up to 500 rpm. If mixing is what is needed at higher speeds and smaller orbits you can find Eppendorf Mixers.

How do I use of sticky pads/sticky tape?

Eppendorf Sticky Pads are recommended for use on our sticky pad platform or our sticky pad adapter kit (M1250-9504). On the S44i universal platform sticky pad can be used directly. The adapter kit is easily fixed to our universal platforms. Any size flask can be used with the sticky pad with a 1 in (2.54 cm) orbit up to 250 rpm, any size flask can be used with the 2 in (5.1 cm) orbit up to 200 rpm. To clean the sticky pad, just wash with soap and water. this will revive" the stickiness, but the sticky pad must be completely dry before replacing on the platform.

What is the heavy load platform ?

The Heavy Duty Platform (M1282-9928) is designed for use in the Innova 44 only. This platform is 0.95 cm thick alluminum plate that is recommended for shaking full loads of flasks greater than 1 L at or above 20 % fill volumes or flasks with baffles.

What is the orbit of a shaker?

An orbital shaker moves the platform in a circular shaking motion. The term orbit describes the diameter of this circular movement. Typical orbit sizes are 1.9 cm, 2.5 and 5 cm.

What orbit shall I take?

An orbital shaker is used to grow microorganisms or cells growing in suspension, like bacteria, yeast, fungal, insect, algae or plant cells. Orbital shaking enables a homogeneous distribution and aeration of the cultures by increasing the oxygen transfer rate (OTR). There are different influencing parameters on shaking performance, e.g. the shaker orbit, the applied speed, the vessel shape, the liquid viscosity and the filling volume. The 2.5 mm orbit is the standard solution which works well for most applications. For some applications, e.g. large vessels > 2 Liter, especially large volume Fernbach flasks or very shear sensitive cells, a larger shaking diameter with the consequence of a reduced speed can be beneficial.

I use two shakers with different orbits. Can I transfer my experimental conditions to a shaker with a different orbit?

Yes, the rpm can be adapted with help of the following formula .

What platform types are offered with the shakers?

Universal platform and a variety of dedicated platforms are available. University platforms offer maximum flexibility having a hole-pattern that allows using virtually any combination of clamps and other accessories. Dedicated platforms come complete with pre-installed clamps of one size. The hole-pattern of the dedicated platform is optimized for the use of one specific size of clamps. It allows in most cased more capacity but can only be used with that size of clamp. Please note that the platform has to be ordered in addition to the shaker.

为什么要定期维护培养箱？

Eppendorf 维护和保养计划提供了一系列可供选择的预防性维护服务，可以确保设备的一致性能以及结果的可靠性。

培养箱需要多久维护一次？

这取决于使用强度，并且因设备负载而异。然而，我们建议每年至少维护一次。

进行维护之前，需要我怎么样处理设备？

首先需要清除设备污染。设备不得锁定且所有样品已从设备中移除。您还应确保设备已经冷却。

Eppendorf 可以提供哪些培养箱维护服务？

您可以在服务与支持/保养和认证板块中找到为相应仪器提供的各种服务产品。

培养箱维护服务价格？

费用取决于型号和所执行的维护。请联系您当地的 Eppendorf 客服。 www.eppendorf.com/contact

请联系您当地的 Eppendorf 服务团队。您可以在我们的主页或资讯材料中找到联系信息。 www.eppendorf.com/contact

IQ 或 OQ 是什么？有什么用？

安装和操作验证；IQ 记录设备已正确交付和安装。 OQ 检查设备是否正常运行。 IQ/OQ 确保设备满足您的质量管理要求，确保设备正常运行且符合制造商指标。

培养箱需要多长时间进行一次 OQ？

公司负责对设备进行技术检查。 Eppendorf 建议定期进行设备验证，具体取决于使用强度。然而，至少应每年进行一次 OQ。

如何清洁/消毒培养箱？

请使用 70% 异丙醇和 30% 蒸馏水混合液作为消毒剂。请严格按照用户手册中描述的清洁指南操作。

培养箱的哪些部件应定期更换？

至少每年更换一次 CO 2 供应管道 HEPA 滤膜以及有自动调零设置的 HEPA 滤膜*。 (* 自动调零滤膜仅用于 R 型培养箱）

我想提高我的技能，你们能够提供哪些类型的培训或在线培训？

请访问 Eppendorf 网页关于 Eppendorf 培训中心的信息或联系您当地的 Eppendorf 客服，以获取更多信息和报价。 www.eppendorf.com/training

Where are the sensors, the access port (for cables) and the gas sample port located in the S41i? Is there an overview of the inner chamber parts (in terms of sensors and in/outlets)?

The S41i has two temperature sensors (one on the top ceiling, one in the back wall below the platform. It has one access port in the back wall to enter cables e.g. from external sensors. It has in the back one inlet for CO2 gas and one outlet for gas sampling. Please see attached document for picture.

为什么要定期维护轨道摇床？

对于有可移动部件的所有产品，磨损总是难以避免的，并且可能长时间影响产品操作一致性。 Eppendorf 产品在设计上可以最大程度减轻磨损，然而定期维护可以在产品性能超出可接受容差范围之外前尽早发现和追踪过度磨损。定期维护也可能是用户的一项监管要求。

轨道摇床需要多久维护一次？

Eppendorf 建议每 12 个月进行一次维护。然而，可能与用户的监管要求存在较大差异，后者可能要求进行更频繁的维护。

轨道摇床维护服务价格？

费用取决于产品型号和所需执行的维护水平。请联系您当地的 Eppendorf 客服获取个体化报价。 www.eppendorf.com/contact

IQ 或 OQ 是什么？有什么用？

安装和操作验证；安装验证记录产品已按照制造商规范和指南正确交付和安装。操作验证检查设备是否正常工作，并且符合制造商规范和指南（因此，只能由设备制造商提供该服务）。 IQ/OQ 主要适用于实施质量管理流程的机构，但也适用于所有用户。

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New Brunswick S41i - CO₂ 培养箱摇床

New Brunswick™ S41i CO2 Incubator Shaker

New Brunswick™ S41i CO2 Incubator Shaker Double-Stack Configuration

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您是否正在寻找一种集成了高温消毒功能并显著降低耗气量的CO₂培养箱摇床？使得清洁更容易并且降低更换CO₂钢瓶的频率 — 无风扇设计并且可堆叠New Brunswick S41i。

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New Brunswick S41i

170 L, 装配内部搁板和触屏控制面板的CO₂生物摇床
可堆叠达2层, 1 (2. optional) 室, 230 V ± 10 %/50/60 Hz (CN), 轨道 2.5 cm (1″)

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目录编号 S41I230014

价格

¥ 282,373.00

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实验结果（例如蛋白表达）的重复性高度依赖于准确的气体环境配置以及防止风扇引发的培养基蒸发？您希望避免摇床污染导致样品损失？并且您不希望在编程、更换 CO₂ 气瓶或清洁方面花费太多时间？

欢迎了解 S41i CO₂ 培养箱摇床提供的智能全方位解决方案，并可以与您现在使用的摇床做一对比。
• 内置New Brunswick摇床的CO₂培养箱
• 高温消毒（120 °C下4 小时）标准
• 全面、快速地分析实时和历史状况
• 易于阅读的大型显示屏，可快速查看多个参数、辅助屏幕、趋势图和诊断分析
• 直观的控制器可让您快速更改环境和警报设置，执行屏幕故障排除和诊断分析
• 72小时连续数据记录，详细记录温度、警报、开门和环境条件
• 密码保护设置和警报设定值

通过VisioNize^® Lab Suite高效管理实验室和设备

您是否希望监测实验室设备并接收设备警报等通知？数字化互联实验室可大大超出您的期望。

获取一系列模块化数字服务，从中选择适合您实验室的服务项目。VisioNize Lab Suite 满足您作为实验室管理人员或科学家进行实验室数字化管理所需的一切，以提高样品安全性，满足合规性需求，提升维护管理水平。

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规格	New Brunswick™ S41i CO2 生物摇床

外部深度（盖/门打开）	144.8 cm (57 英寸)
容量	170 L (6.0 ft³)
搁板数量	1 (2. optional)
定时器	持续运行
电源	230 V ± 10 %, 50/60 Hz
尺寸（长x宽x高）	87.5 × 73 × 85 cm / 34.4 × 28.7 × 33.5 in
产品重量	154 kg / 340 lb
高温消毒 (HTD)	120 °C
密封内玻璃门	标准
分段门方案	–
温度控制增量	±0.1 °C
37 °C 时的温度稳定性	±0.1 °C
温度范围	环境温度 +4 °C 至 50 °C
声光报警	是
温度均一性	37°C下±0.25 °C（锥形瓶培养基中）
CO₂ 范围	0.2 – 20 %
可用程序模式	连续温度与转速控制
CO₂ 控制增量	±0.1 %
5 % CO₂ 时的 CO₂ 稳定性	±0.2 %
驱动装置	三偏心轴平衡驱动
CO₂ 均一性	±0.1 %
搁板数量（标准/最大）	1/2
数据导出接口	Ethernet
台上 (B)，台下 (U)，落地 (F) 或叠放 (S)	U, F, S (x2)
访问端口	1
板载数据记录	标准
BMS 继电器	标准
存储卡	断电后重启自动运行记录参数设置
马达类型	固态无碳刷马达
摇床板尺寸	36 × 61 cm (14 × 24 in)
正在打开门	向前方打开
速度范围	25 – 400 rpm（叠放时 25 - 250/400 rpm 适用于顶部/底部设备）
cETLus 和 CE 标志	是
容量	中型
带孔搁板	标准
远程设备监视和通知 (VisioNize^®)	VisioNize^®框已启用
振荡	标配
温度范围	环境温度以上 4 °C 至 50 °C
温控准确性	±0.2 °C
无缝箱体	标准
精细气体减压阀	标准

搅拌
轨道	2.5 cm (1″)