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- Challenges and Chances: A Review of the 1st Stem Cell Community Day
- Summertime, and the Livin’ Is Easy…
- Follow-on-Biologics – More than Simple Generics
- Bacteria Versus Body Cells: A 1:1 Tie
- Behind the Crime Scene: How Biological Traces Can Help to Convict Offenders
- Every 3 Seconds Someone in the World Is Affected by Alzheimer's
- HIV – It’s Still Not Under Control…
- How Many Will Be Convicted This Time?
- Malaria – the Battle is Not Lost
- Physicians on Standby: The Annual Flu Season Can Be Serious
- At the Forefront in Fighting Cancer
- Molecular Motors: Think Small and yet Smaller Again…
- Liquid Biopsy: Novel Methods May Ease Cancer Detection and Therapy
- They Are Invisible, Sneaky and Disgusting – But Today It’s Their Special Day!
- How Many Cells Are in Your Body? Probably More Than You Think!
- What You Need to Know about Antibiotic Resistance – Findings, Facts and Good Intentions
- Why Do Old Men Have Big Ears?
- The Condemned Live Longer: A Potential Paradigm Shift in Genetics
- From Research to Commerce
- Chronobiology – How the Cold Seasons Influence Our Biorhythms
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Influence of Physical Parameters on the Dispensed Volume of Air-Cushion Pipette
生命科学の探究
- メンテナンスとキャリブレーション
- ピペッティング技術
- エッセー
- 心を動かすサイエンス
The surrounding in the laboratory e.g., humidity, altitude and temperature, as well as the liquid properties of a sample itself like viscosity, volatility or density have a tremendous influence on the pipetting result. Each factor influences the pipetting volume and with this the experimental result in a different way. So a liquid temperature other than 22 °C already leads to less or excessive volume delivery. Or a dense liquid like phosphoric acid leads to constant insufficient liquid volume. All factors have to be considered during daily pipetting tasks and especially during calibration of a pipette. So consider our recommendations to improve your daily pipetting work.
Impact on the systematic accuracy of a pipette with liquid densities deviating from the density of water. When using dense liquids the pipette needs to be adjusted for proper volume delivery.
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Liquid temperatures other than 22 °C lead to systematic measurement deviations if forward pipetting technique is performed. Therefore another pipetting technique like reverse pipetting or a different pipette type like a direct replacement system should be considered.
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Pipettes are calibrated to an altitude of 0 m sea level. Different altitudes lead to higher systematic errors and can only be beaten by a permanent adjustment of the pipette.
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If you want to read more about the influence of physical parameters on the dispensed volume of air-cushion pipette, please download our Userguide!
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