Are Eppendorf products free of RNA?

All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.

What is the meaning of the PCR Clean seal?

For molecular-biological requirements Eppendorf offers a new standard for uniform purity specifications. All consumables marked with the "Certified PCR Clean” label are guaranteed free of human DNA, DNase, RNase and PCR inhibitors.

Why are there double labels on the folding boxes, as "PCR clean" + "Sterile" or "PCR clean" + "Protein-free"?

Due to the modularization of purity grades, some products are labeled with more than one purity grade symbol. For example, the epDualfilter T.I.P.S. has the double seal "PCR clean "+ "Sterile" and the UVette the double seal "PCR clean" + "Protein-free" on the packing. The certification remains unaffected by the modularization.

Are lot specific certificates for the purity grades available in the internet?

Yes, the lot specific purity certificates, so-called Test report, can be downloaded at the certificates homepage .

Can products of the previous and new modular purity grades (with new seals) be used simultaneously in one application?

Yes, if no batch attachment is required for the application, products of the old and new modular purity grades can be used, as their specifications are the same.

What distinguishes the DNA LoBind Tube from a "Standard" Safe-Lock Tube?

In comparison to a Safe-Lock Tube, the DNA LoBind Tube shows significantly less DNA bonding affinity. This effect is most apparent with high salt concentrations and when working with short DNA fragments. The DNA LoBind Tube is therefore especially suitable for applications that should be carried out with the minimum loss of nucleic acid material.

Do the Eppendorf LoBind Tubes have the same material durability as all other micro test tubes from Eppendorf?

The Eppendorf LoBind Tubes completely satisfy the specifications of the Eppendorf Standard Safe Lock tubes with reference to centrifugation stability (exception: Protein LoBind Tube), lid tightness, pop up safety and chemical resistance. (See Application No. 56: The best material for original Eppis.)

Are the Eppendorf LoBind Tubes coated?

Not the Eppendorf LoBind Tubes are neither siliconized nor coated. The low binding affinity results from of a special type of polypropylene and the optimized manucturing process.

What distinguishes the Protein LoBind Tube from a "Standard" Safe-Lock Eppendorf tube?

The Protein LoBind Tube shows, in comparison to a Safe-Lock Tube, significantly less protein binding affinity. The Protein LoBind Tube is therefore especially suitable for applications with the lowest quantities of protein.

Are the Eppendorf LoBind Tubes autoclavable?

Please note that only the LoBind Tubes with snap can are autoclaveable when opened: 121°C, 20 min. LoBind Tubes with screw cap are not autoclaveable.

Are Eppendorf tubes and plates (also LoBind variants) resistant to 100% acetonitrile?

Eppendorf tubes and plates including LoBind variants are resistant against 100% acetonitrile only in room temperature conditions. Above 40°C and prolonged exposure (days) may lead to partial damage and is not recommended. For lower acetonitrile concentrations the resistance parameters will be better so prolonged exposure and higher temperatures may be possible. This must be however tested by the customer.

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DNA LoBind^® Tubes

Sample being pipetted from elongated ep T.I.P.S.® to Eppendorf LoBind® deepwell plate

Scientist pipetting into Eppendorf 5 mL microtubes® stored in rack

DNA LoBind® tubes - 0.5 mL, 1.5 mL, 2 mL and 5 mL with closed lid

Eppendorf LoBind® tube with open lid and volume marker

Eppendorf LoBind® tubes - 0.5 mL, 1.5 mL, 2 mL and 5 mL

Small section of Eppendorf DNA LoBind® microplate

製品情報

核酸回収を最大化してください！Eppendorf LoBind^® Tubesは試料表面吸着を大幅に軽減し、DNAおよびRNA分子の回収を確実に最大化します。試料を調製して、精密な核酸を長期保管するのに最適なソリューションです。

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製品情報

アプリケーション

特徴

Eppendorf LoBind^® Tubesで試料の損失を最小化し、精密な核酸の回収を最大化してください。優れた製造テクノロジーとハイクオリティのポリプロピレンを組み合わせて、LoBind^®チューブはDNAおよびRNA分子のほぼ100 %の回収を表面コーティングなしで提供します。
確かなことはEppendorf LoBind^® Tubesが最高のクオリティで、法医学分析、マイクロアレイ、そして次世代シーケンシング（NGS)を含めたすべての用途に適合することです。LoBind^®チューブは独立したラボによる厳しいバッチテストとDNA、DNase、RNase、PCR inhibitorが含まれていないことを保証する証明書を受けています。

あらゆる問題のためのEppendorf LoBind^®ソリューション！

Eppendorf DNA LoBind^®にはさまざまなフォーマットがあります - 標準チューブからマイクロプレートやディープウェルプレートまで - 試料低容量でももっと大きい処理量でもお客様特殊のニーズに対応するのを助けるためです。

未曾有のDNA回収率

Eppendorf LoBind^® Tubes他メーカーと比較すると、培養温度と時間に関係なく、 DNAの最大99 %を回収します。グラフは、さまざまな培養条件に従った Eppendorf LoBind^® Tubesと他メーカーのチューブにおけるDNA 回収率（2.5 M NaCl/TEバッファの0.2 ng/µL DNA断片 (130 bp, 32P ラベル付き））。

Eppendorf LoBind^® Tubesは、他メーカーの低吸着チューブより大きい回収の可能性を提供します。リアルタイムPCR絶対定量化のための標準の調製と保管におけるEppendorf LoBind^® Tube一貫性の評価は、DNAのLoBind^® Tubesに対する低吸着が標準カーブのクオリティとDNA定量化を改善することを解析試料において示しました。

画像：DNA標準の調製：ゲノムDNAストック溶液は他メーカーの低吸着チューブまたはEppendorf DNA LoBind^® Tubesで連続的に10倍に希釈しました。各希釈の22 µLの分割量はリアルタイムPCRで分析する前に –80 °Cで24時間保管しました。Eppendorf LoBind^® Tubesで保管した試料は他メーカーと比べてPCR効率が極めて高いです。詳細については、アプリケーションノート226を参照してください。