For which applications may I use Eppendorf Conical Tubes ® 25 mL?

In general, all applications where standard conical tubes 15mL and 50 mL are routinely used. The Eppendorf Conical Tubes ® 25 mL close a gap between 15 mL and 50 mL conical tubes and additionally offer in this format a unique snap cap – the so called SnapTec™ cap - which markedly improves handling. This makes the Eppendorf Conical Tubes ® 25 mL ideally suited for all cell/tissue culture applications including splitting cells, media change, transfections, etc. Also assay preparation and biomolecule purification protocols (gDNA, plasmid DNA, RNA, proteins), which require organic solvent extractions and multiple centrifugation steps may be successfully performed. They are also ideally suited for general reagent / sample handling and space-saving storage.

Is it necessary to buy new equipment to enable the use of Eppendorf Conical Tubes 25 mL within existing lab periphery?

No. The bottom shape / diameter of the Eppendorf Conical Tubes 25 mL is virtually the same with standard conical 50 mL tubes. With the respective height adapter (provided as Eppendorf accessory), the Eppendorf Conical Tubes 25 mL will be compatible with a wide range of standard laboratory equipment: centrifuges, mixers, etc., additionally tube racks, storage boxes and single-tube stands (skirts) are available for lab bench work, weighing and storage. For detailed accessories description please refer to www.Eppendorf.com .

Is it possible to store samples in 25 mL Eppendorf Conical Tubes in the freezer?

Yes, the tubes can be stored down to -86 °C.

How resistant are the Eppendorf Conical Tubes ® 25 mL to chemicals?

The tubes are made of high quality, transparent polypropylene and resistant to a majority of commonly used chemical substances in the laboratory. An overview is provided in Application Note No. 56. In case a substance/chemical is not listed there, please contact our local contact.

Which purity grades and LoBind / Amber variants are available for Eppendorf Conical Tubes 25 mL?

The Eppendorf Conical Tubes 25 mL are available in the purity grades: Eppendorf Quality, PCR clean and Sterile/Pyrogen-free. For general and lot-specific certificates please refer to Eppendorf website eppendorf.com/certificates. The DNA and Protein LoBind as well as Amber variants of Eppendorf Conical Tubes ® 25 mL will be available within short term as well.

Correct opening and closing of the snap Lid (SnapTec ® ).

The snap cap of the Eppendorf Conical Tubes ® 25 mL is much larger than in other tube formats and may require some short learning of correct closing/opening at the beginning. Opening: press the safety tab from below and steady press upwards. In case of pressure buildup (organic solvents, higher temp incubations) make sure you slightly hold the lid with your thumb and don’t let the lid to pop out rapidly. Closing: press down the lid in the front area and make sure the safety tab clicks and is locked at right angle to the tube. Improperly locked tab may cause leaking.

Are Eppendorf tubes and plates (also LoBind variants) resistant to 100% acetonitrile?

Eppendorf tubes and plates including LoBind variants are resistant against 100% acetonitrile only in room temperature conditions. Above 40°C and prolonged exposure (days) may lead to partial damage and is not recommended. For lower acetonitrile concentrations the resistance parameters will be better so prolonged exposure and higher temperatures may be possible. This must be however tested by the customer.

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Eppendorf Conical Tubes 25 mL

Conical Tube 25mL single-handed operation

Tube Rack 5, 15, 25, 50 mL top view, with medium

Eppendorf Freezer storage box family for 6 different tube volume classes

ACT label of sustainability certification for Eppendorf 25 mL conical tubes (SnapTec, sterile)

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全新高度 – Eppendorf Conical Tubes 25 mL！
Eppendorf 离心管大家族中的最新成员填补了传统 15 mL 和 50 mL 锥形管之间的空白。这些离心管品质出众，且高度经过优化设计，是细胞生物学应用的理想工具，例如从T75培养瓶汇合培养基，中量规模细菌培养或诸如缓冲剂制备、储存和运输等重叠应用。

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特征

探索全新规格的Eppendorf Conical Tubes 25 mL离心管！全新的Eppendorf Conical Tubes 25 mL离心管将锥形离心管产品推向新的高度。以往在必须制备、离心、混合或储存 15 mL 以上 50 mL 以下体积的样品时，除了使用 15 mL 或 50 mL 螺旋盖锥形管之外，科研人员通常别无选择。全新的 Eppendorf Conical Tubes 25 mL 离心管可以解决这个难题。它们的直径与 50 mL 锥形管相同，但长度更短。因此与 50 mL 锥形管相比，移液器的插入深度更浅。全新的 25 mL 离心管可以配备螺旋盖或创新性 SnapTec^® 盖。这种管盖解决方案已获得专利，代表了锥形管领域的一种独特弹扣盖。
高纯净原生材质，生产过程中不会添加润滑剂、塑化剂和抑菌剂等添加剂，确保样品高度纯净。离心稳定性高达17,000 x g，盖密封性从 -86 °C至高达 100 °C以及确保优化样品和颗粒可见性。

由于这种全新的 Eppendorf 离心管与 50 mL 锥形管的直径相同，可以兼容现有实验室设备。可以提供多种系统组件，诸如离心用适配器、Eppendorf SmartBlock™、MixMate^® 试管架、储存盒和单离心管架。

全新管盖设计：

• 人性化设计的管盖经过几何形处理侧面平坦，便于操作（打开/关闭），可以单手操作
• 确保管盖稳定朝上放置，并减少污染几率
• 高质量材料和精密模制，确保高度气密性并减少样品损失

高离心耐受力

能够缩短离心时间并确保样品、用户和设备安全。

高纯度级离心管，适应于无菌细胞应用以及核酸应用：
• 无菌、无热原、无 DNase、RNase 和 DNA（人类和细菌）
• 每批均经过检测和认证

实验室Eppendorf 25 mL=耗材及其包装材料的处置。

如今，材料回收变得愈发重要。例如，欧洲几乎100%回收所有纸板材料。Eppendorf Conical Tubes 25 mL的包装由不同材料制成，其中大部分为纸板。
Eppendorf耗材和Eppendorf Tubes的包装至少采用70%的可回收纸板。
请收集离心管的纸板包装材料，支持我们回收有价值原材料的全球可持续发展倡议。将包装材料放在您所在单位和地区的指定收集容器内。关于非灭菌耗材和聚乙烯(PE)/聚对苯二甲酸乙二醇酯（PE/PET）的聚乙烯包装薄膜，我们建议选择一个可回收PE材料的专业 PE/PET 回收合作伙伴。请联系您所在机构或当地废物管理公司，了解贵公司的可用回收方案。
离心管在使用后可能受到污染。这种污染可能导致生物危害、化学危害，甚至是核危害。因此，这些容器的回收工作可能非常具有挑战性。在许多国家，由于法律限制，危险废物必须焚烧。然而，焚烧受污染的实验室耗材可以回收能量和热量。
为了减少危险塑胶垃圾，您应该至少使用两种废物容器：一种用于收集危险废物，一种用于收集非危险废物。这两种废物类型的定义可能取决于当地法规。您应该针对实验室工作人员开展相关培训。请联系当地的生物安全官员和/或当地的废物管理部门，了解更多本地废物回收方案。
我们强烈推荐经过认证的本地回收合作伙伴。采用“本地”处理方式将减少运输带来的影响。选用“认证”回收公司，能够以安全和可持续的方式处理实验室废物。

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规格	25 mL，卡扣盖，Eppendorf 优质级	25 mL，卡扣盖，PCR洁净级	25 mL，卡扣盖，无菌级	25 mL，螺旋盖，Eppendorf 优质级	25 mL，螺旋盖，PCR洁净级	25 mL，螺旋盖，无菌级

ACT 标签	是	是	是	是	是	是
产品类型	耗材	耗材	耗材	耗材	耗材	耗材
纯度级别	Eppendorf Quality优质级	PCR 洁净级	无菌、无热原, 无RNase和DNase, 无DNA	Eppendorf Quality优质级	PCR 洁净级	无菌、无热原, 无RNase和DNase, 无DNA
包装材料	200 个 (5 包 × 40 个)	200 个 (5 包 × 40 个)	150 个 (6 包 × 25 个)	200 个 (4 包 × 50 个)	200 个 (4 包 × 50 个)	200 个 (8 包 × 25 个)
高温高压灭菌	是	是	是	否	否	否
直径 (mm)	30 mm	30 mm	30 mm	30 mm	30 mm	30 mm
光保护	否	否	否	否	否	否
颜色	无色	无色	无色	无色	无色	无色
材质	离心管和盖：聚丙烯	离心管和盖：聚丙烯	离心管和盖：聚丙烯	离心管：聚丙烯（原生材质，符合 USP 6 级标准）螺旋盖：高密度聚乙烯 (HDPE)	离心管：聚丙烯（原生材质，符合 USP 6 级标准）螺旋盖：高密度聚乙烯 (HDPE)	离心管：聚丙烯（原生材质，符合 USP 6 级标准）螺旋盖：高密度聚乙烯 (HDPE)
体积	25 mL	25 mL	25 mL	25 mL	25 mL	25 mL
最大 RCF	17000 × g	17000 × g	17000 × g	17000 × g	17000 × g	17000 × g
工作温度	-86 °C –  100°C	-86 °C –  100°C	-86 °C –  100°C	-86 °C –  100°C	-86 °C –  100°C	-86 °C –  100°C
离心稳定性	高达 17,000 x g¹⁾	高达 17,000 x g¹⁾	高达 17,000 x g¹⁾	高达 17,000 x g¹⁾	高达 17,000 x g¹⁾	高达 17,000 x g¹⁾
底部形状	锥形	锥形	锥形	锥形	锥形	锥形