Are Eppendorf products free of RNA?

All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.

What is the meaning of the PCR Clean seal?

For molecular-biological requirements Eppendorf offers a new standard for uniform purity specifications. All consumables marked with the "Certified PCR Clean” label are guaranteed free of human DNA, DNase, RNase and PCR inhibitors.

Why are there double labels on the folding boxes, as "PCR clean" + "Sterile" or "PCR clean" + "Protein-free"?

Due to the modularization of purity grades, some products are labeled with more than one purity grade symbol. For example, the epDualfilter T.I.P.S. has the double seal "PCR clean "+ "Sterile" and the UVette the double seal "PCR clean" + "Protein-free" on the packing. The certification remains unaffected by the modularization.

Are batch-specific certificates for the new purity grades available in the internet?

Yes, the batch-specific purity grades can be downloaded at our certificates homepage .

Can products of the previous and new modular purity grades (with new seals) be used simultaneously in one application?

Yes, if no batch attachment is required for the application, products of the old and new modular purity grades can be used, as their specifications are the same.

What distinguishes the DNA LoBind Tube from a "Standard" Safe-Lock Tube?

In comparison to a Safe-Lock Tube, the DNA LoBind Tube shows significantly less DNA bonding affinity. This effect is most apparent with high salt concentrations and when working with short DNA fragments. The DNA LoBind Tube is therefore especially suitable for applications that should be carried out with the minimum loss of nucleic acid material.

Do the Eppendorf LoBind Tubes have the same material durability as all other micro test tubes from Eppendorf?

The Eppendorf LoBind Tubes completely satisfy the specifications of the Eppendorf Standard Safe Lock tubes with reference to centrifugation stability (exception: Protein LoBind Tube), lid tightness, pop up safety and chemical resistance. (See Application No. 56: The best material for original Eppis.)

Are the Eppendorf LoBind Tubes coated?

Not the Eppendorf LoBind Tubes are neither siliconized nor coated. The low binding affinity results from of a special type of polypropylene and the optimized manucturing process.

What distinguishes the Protein LoBind Tube from a "Standard" Safe-Lock Eppendorf tube?

The Protein LoBind Tube shows, in comparison to a Safe-Lock Tube, significantly less protein binding affinity. The Protein LoBind Tube is therefore especially suitable for applications with the lowest quantities of protein.

Are the Eppendorf LoBind Tubes autoclavable?

Please note that only the LoBind Tubes with snap can are autoclaveable when opened: 121°C, 20 min. LoBind Tubes with screw cap are not autoclaveable.

Are Eppendorf tubes and plates (also LoBind variants) resistant to 100% acetonitrile?

Eppendorf tubes and plates including LoBind variants are resistant against 100% acetonitrile only in room temperature conditions. Above 40°C and prolonged exposure (days) may lead to partial damage and is not recommended. For lower acetonitrile concentrations the resistance parameters will be better so prolonged exposure and higher temperatures may be possible. This must be however tested by the customer.

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DNA LoBind^® Tubes

Sample being pipetted from elongated ep T.I.P.S.® to Eppendorf LoBind® deepwell plate

Scientist pipetting into Eppendorf 5 mL microtubes® stored in rack

DNA LoBind® tubes - 0.5 mL, 1.5 mL, 2 mL and 5 mL with closed lid

Eppendorf LoBind® tube with open lid and volume marker

Eppendorf LoBind® tubes - 0.5 mL, 1.5 mL, 2 mL and 5 mL

Small section of Eppendorf DNA LoBind® microplate

产品信息

尽可能提高核酸回收率！ Eppendorf LoBind^® Tubes可显著减少样品和表面的结合，以确保尽可能提高DNA和RNA分子回收率。低 DNA 吸附管是用于珍贵核酸制备和长时间样品储存的更佳选择！

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产品信息

用途

特征

使用Eppendorf LoBind^® Tubes尽可能减少样品损失并尽可能提高珍贵核酸的回收率。结合卓越的制造技术和优质聚丙烯，LoBind^®离心管提供几乎100 %的DNA和RNA分子回收率，无需表面涂层。
请放心，Eppendorf LoBind^® Tubes的质量优良，适用于包括法医分析、微阵列和下一代测序（NGS）在内的各种用途。 LoBind^®离心管经过严格的批量测试和独立实验室的认证，以确保其不含DNA、DNA酶、RNA酶和PCR抑制剂

Eppendorf LoBind^®溶液可解决所有问题！

Eppendorf DNA LoBind^® 有各种形式可用 - 从标准离心管到微孔板和深孔板 - 可满足您的具体需求，无论是低样品量还是需要更高的处理能力。

与竞争对手的离心管相比，具有无可比拟的DNA回收率

Eppendorf LoBind^® Tubes回收多达99 %的 DNA。图形显示了在不同培育条件下， in Eppendorf LoBind^® Tubes和竞争对手离心管的DNA 回收率（0.2 ng/µL DNA 片段（130 bp，32P 标记）在 2.5 M NaCl/TE 缓冲液中）。

与竞争对手的低吸附力离心管相比，Eppendorf LoBind^® Tubes提供更高的回收能力。 Eppendorf LoBind^® Tube评估与PCR绝对定量标准品制备和储存一致，显示DNA吸附至LoBind^® Tubes的数量较低，这提高了标准曲线的质量和分析样品中的DNA定量。

图像： DNA标准板制备：基因组DNA储备液在竞争对手的低吸附力离心管或Eppendorf DNA LoBind^® Tubes试管中连续稀释10倍。在实时PCR分析之前，将每种稀释液的22 µL等分试样在–80°C下保存24小时。与竞争对手的产品相比，存放在Eppendorf LoBind^® Tubes中的样品有更高的PCR效率。更多信息见应用文献第 226 期。