Are Eppendorf products free of RNA?

All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.

What is the meaning of the PCR Clean seal?

For molecular-biological requirements Eppendorf offers a new standard for uniform purity specifications. All consumables marked with the "Certified PCR Clean” label are guaranteed free of human DNA, DNase, RNase and PCR inhibitors.

Why are there double labels on the folding boxes, as "PCR clean" + "Sterile" or "PCR clean" + "Protein-free"?

Due to the modularization of purity grades, some products are labeled with more than one purity grade symbol. For example, the epDualfilter T.I.P.S. has the double seal "PCR clean "+ "Sterile" and the UVette the double seal "PCR clean" + "Protein-free" on the packing. The certification remains unaffected by the modularization.

Are lot specific certificates for the purity grades available in the internet?

Yes, the lot specific purity certificates, so-called Test report, can be downloaded at the certificates homepage .

Can products of the previous and new modular purity grades (with new seals) be used simultaneously in one application?

Yes, if no batch attachment is required for the application, products of the old and new modular purity grades can be used, as their specifications are the same.

Are Eppendorf tubes and plates (also LoBind variants) resistant to 100% acetonitrile?

Eppendorf tubes and plates including LoBind variants are resistant against 100% acetonitrile only in room temperature conditions. Above 40°C and prolonged exposure (days) may lead to partial damage and is not recommended. For lower acetonitrile concentrations the resistance parameters will be better so prolonged exposure and higher temperatures may be possible. This must be however tested by the customer.

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DNA LoBind^® Plates

Small section of Eppendorf DNA LoBind® microplate

Deep well Eppendorf LoBind® plate with blue border

Barcoded Eppendorf LoBind® plate in deep well format

Sample being pipetted from elongated ep T.I.P.S.® to Eppendorf LoBind® deepwell plate

产品信息

我们的创新性DNA LoBind 技术减少样品与表面的结合，以确保尽可能回收核酸，并为样品制备和长期储存提供理想的解决方案。

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产品信息

用途

特征

使用DNA LoBind^® Plates更大限度地提高核酸回收率！专用制造技术和精选的聚丙烯相结合，确保 DNA/RNA 分子的回收率接近 100 % — 无需表面涂层，避免了样品污染的风险。这使Eppendorf LoBind Plates 非常适用于非常注重样品回收的用途 - 例如在法医、微阵列和 NGS 应用中制备长期储存的核酸。

高质量Eppendorf DNA LoBind^®产品

产品包括吸附管、微孔板和深孔板在内的一系列形式，可用于帮助您回收核酸，满足各种样品量和处理量的不同应用需求。

优异的DNA回收率

Eppendorf DNA LoBind^® Tubes与竞争对手的离心管相比，DNA回收率显著更高。在达到评估样品回收率的条件之前，在各种条件下培养DNA（0.2 ng/µL DNA 片段（130 bp，32P 标记）在 2.5 M NaCl/TE 缓冲液中）。 Eppendorf DNA LoBind Tubes显示的样品回收率高达99 %，与培育温度或时间无关。

实现量化过程的一致性

Eppendorf LoBind^® Tubes与低吸附力的竞争对手产品相比，提供更大的回收能力。 Eppendorf LoBind离心管评估与PCR绝对定量标准品制备和储存一致，显示DNA吸附至LoBind离心管的数量较低，这提高了标准曲线的质量和分析样品中的DNA定量。

图像： DNA标准板制备：基因组DNA储备液在竞争对手的低吸附力离心管或Eppendorf DNA LoBind^® Tubes中连续稀释10倍。在实时PCR分析之前，将每种稀释液的22 µL等分试样在–80°C下保存24小时。与竞争对手的产品相比，存放在Eppendorf LoBind^® Tubes中的样品有更高的PCR效率。更多信息见应用文献第 226 期。