What is the meaning of the red knob on the rotor lid?

A fixed-angle rotor with a red knob on the rotor lid indicates aerosol-tightness.

Can the Centrifuge 5427 R be opened in the event of a power failure?

Yes, in the event of a power failure, the unit can be opened by means of the rotor key. Before the centrifuge is opened, always remove the mains plug from the plug socket and wait for the rotor to come to a standstill. For the emergency release of the centrifuge lid, the small plastic cap on the right front corner of the 5427 R has to be removed. Then insert the rotor key into the nut underneath the cap and open the lid latches by turning in anticlockwise direction.

How can the Centrifuge 5427 R be cleaned?

The Centrifuge 5427 R can be cleaned with neutral detergents. For disinfection, we recommend alcohol or alcoholic disinfectants. Make sure that the relevant legal requirements and directives are observed when choosing the disinfection method.

Sometimes my Centrifuge 5427 R displays "EP". What does that mean?"

EP" is displayed when the automatic standby function is active. If the centrifuge remains unused for more than 15 min., the standby function is activated, indicated by "EP" on the display. By using any key, the centrifuge is re-activated and ready to use.

Can the Centrifuge 5427 R automatically recognize an installed rotor?

The Centrifuge 5427 R has an automatic rotor recognition system. A newly installed rotor is recognized at the beginning of the run. The maximum speed of each rotor is automatically limited, if necessary, to prevent over-speeding of the rotors.

What benefits do I gain from the centrifugation of my samples with the swing bucket rotor?

Swing-bucket rotors are particularly advantageous for gradient centrifugation applications as horizontal layers are formed that remain in this position at the end of centrifugation (e.g. blood/Ficoll).

What is the benefit of a so-called Kit rotor?

When working with nucleic acid purification kits with spin columns by certain manufacturers, sometimes tube lids cannot be closed any more. This means you have to centrifuge with open tube lids. These tube lids might rip off during the stress of centrifugation and might damage the centrifuge. In order to prevent this, the kit rotor with the raised rotor rim was designed. Open tube lids, supported against the rim, will not rip off during centrifugation. Another feature of this rotor is the enlarged distance between the rotor bores. The enlarged distance between the bores offers a very convenient handling when loading / unloading the rotor. Especially when working with long spin columns (e. g. from Princeton Separations) you will need a larger distance between the bores to load the rotor fully and make use of the complete rotor capacity.

What tubes can be centrifuged with the Centrifuge 5427 R?

Due to the different rotors and adapters, a wide variety of tubes can be centrifuged with the Centrifuge 5427 R: • Micro test tubes (0.5 - 5 mL) • Cryo-Vials • HPLC-Vials • PCR strips and 0.2 mL PCR tubes • Spin columns (1.5/2.0 mL) • Microtainer (0.6 mL)

In which rotors can I centrifuge 0.2 mL PCR tubes?

Up to 48 0.2 mL PCR tubes (or 6 x 8-tube PCR strips) can be centrifuged in the PCR rotor F-45-48-5-PCR. Furthermore, 0.2 mL PCR tubes can be used in each rotor for 1.5/ 2.0 mL micro test tubes using an adapter

In which rotors can I centrifuge 0.5 mL micro test tubes?

0.5 mL micro test tubes can be centrifuged in all rotors for 1.5/ 2.0 mL micro test tubes using an adapter.

In which rotors can I centrifuge 5 mL micro test tubes?

Up to 16x 5 mL micro test tubes can be centrifuged in the aerosol-tight rotor FA-45-12-17.

In which rotor can I centrifuge spin columns?

Spin columns or small filtration columns, for example for centrifugation of DNA or RNA, can be used in all rotors for 1.5/2.0 mL micro test tubes. In particular, however, the kit rotor F-45-24-11-Kit is specially suitable for such spin columns, as open tube lids are supported by the raised edge of this rotor type and are no longer torn off (e.g. during elution of the sample from the column into a micro test tube)

The "Fast Temp" function is a temperature control run of the Centrifuge 5427 R without samples and with a rotor and temperature-specific rotational speed in order to quickly bring the rotor chamber, incl. the rotor, buckets and adapters, to the set nominal temperature. In order that the samples can be centrifuged from the beginning on at the desired temperature, one starts this temperature control run during sample preparation by pressing the "Fast Temp" key. The display shows the current temperature and the g-force (rcf) / rotational speed (rpm) as well as "FT" as symbol for a "Fast Temp" temperature run. You can find a more detailed description in the operating instructions of the Centrifuge 5427 R.

What is the temperature range of the Centrifuge 5427 R?

With the Centrifuge 5427 R it is possible to set the temperature within a range from -11 °C to +40 °C.

Does the Centrifuge 5427 R has an energy saving function?

Yes, with "ECO shut-off" the continuous cooling is switched off if the centrifuge is not used for longer than the preset time. With the default settings, the continuous cooling ends after 8h. In the menu setting "TEMP" the continuous cooling can be limited to 1h, 2h or 4h.

For what applications are aerosol-tight rotors important?

Aerosol-tight rotors are always recommended or even prescribed when working with hazardous or potentially hazardous substances. These can, for example, be viruses, pathogenic bacteria, potentially infectious blood samples or radioactive sample material.

Why are the standard rotors in the micro centrifuges 5418 R, 5420, 5424 R, 5425, 5427 R, 5430 and 5430 R aerosol-tight?

At Eppendorf, the safety of the user always has the highest priority. That is why we now fit our centrifuges with aerosol-tight rotors as standard – at no extra charge.

What kind of cooling liquid is used for the Centrifuge 5427 R?

The cooling system operates with 75g of the "green" cooling liquid R290, also known as propane.

What is the SOFT function?

The SOFT function of the Centrifuge 5427 R allows slower and gentler acceleration and braking compared to the normal acceleration and braking times. This setting is especially suited for applications like e.g. density gradient centrifugation where remixing of phases must be prevented.

Are there any additional safety features implemented due to R290?

Like all our cooling systems, the cooling system of Centrifuge 5427R is a closed system which has no leakage of cooling liquid. For the unlikely case of leakage two additional safety measures are implemeted: All potentially sparks emitting components are shielded, before the centrifuge is ready for operation, a ventilation system takes care to exchange the air in the centrifuge. Therefore, you might notice a few more seconds prolonged initialization phase.

Do I have to take special cautions during transport, storage or installation of the Centrifuge 5427 R due to the green cooling liquid?

No special transport requirements are necessary as only 75 g propane are used. This also means that the centrifuge can be transported by air freight. The room where the centrifuge 5427 R is stored and installed needs to have a volume of minimal 10 m³. In case that more than one device is installed, the requirement stays to 10 m³. During operation leave a safety clearance of 50 cm around the device and at least 30 cm to the rear of the device.

What is the temperature range of the Centrifuge 5427 R?

With the Centrifuge 5427 R it is possible to set the temperature within a range from -11 °C to +40 °C.

How do I recognize aerosol-tight rotors?

Firstly, the aerosol-tight rotors can be recognized by their designation abbreviation “FA” or “AT”; secondly, Eppendorf has color-coded the aerosol-tight rotors. All aerosol-tight rotors have a red rotor lid screw, and a red ring is printed on to the rotor shaft.

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Centrifuge 5427 R

High-throughput microcentrifuge for up to 48 x 1.5/2 mL tubes

Woman closing microcentrifuge lid with one finger touch

Centrifuge 5427 R offers a great versatility with nine different rotor options

Microcentrifuge rotor for up to 48 x 1.5/2 mL microtubes

Microcentrifuge rotor for up to 30 x 1.5/2 mL microtubes

Microcentrifuge swing-bucket rotor for 24 x 1.5 mL tubes

Microcentrifuge rotor for 24 x 1.5/2 mL spin-colum tubes

Microcentrifuge rotor for 24 x 1.5/2 mL tubes

Microcentrifuge rotor for 5 mL Eppendorf tubes

ACT certificate for sustainability score of Eppendorf Centrifuge 5427 R

ACT certificate for sustainability score of Eppendorf Centrifuge 5427 R with rotor FA45-30-11

ACT certificate for sustainability score of Eppendorf Centrifuge 5427 R with rotor FA45-48-11

ACT certificate for sustainability score of Eppendorf Centrifuge 5427 R without rotor

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Eppendorf Centrifuge 5427 R 是一款用于高通量研究应用的高速冷冻微型离心机。搭载48孔双排转子，比24孔标准微型离心机的试管容量高出一倍，并且采用天然制冷剂，提高实验室工作的可持续性。

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2 组合套装

Centrifuge 5427 R

旋钮式, 冷冻型, 含转子 FA-45-48-11
ACT, 230 V/50 – 60 Hz (CN)

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目录编号 5429000292

Centrifuge 5427 R

旋钮式, 冷冻型, 含转子 FA-45-30-11
ACT, 230 V/50 – 60 Hz (CN)

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目录编号 5429000392

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特征

全球变暖及其后果是当今世界所面临的最大挑战之一。在过去，氢氟烃曾在空调等冷却系统以及实验室离心机或冰箱中用作制冷剂，但由于其化学结构，可能会导致全球变暖。为了保护子孙后代赖以生存的星球，改用更环保、更“绿色”的制冷剂 — 碳氢化合物非常重要。这些天然制冷剂具有与二氧化碳类似的全球变暖潜能。为了更好地在为实验室环境提供支持，Eppendorf 如今上市了产品组合中第一台使用天然绿色制冷剂的离心机，以保护您的样本和地球环境。
这款小型冷冻离心机Centrifuge 5427 R为满足高通量研究应用而设计。配合双排48孔气密性固定角转，运行更为安全可靠，运行时间也大为缩短，可应对实验室里各种离心挑战，有效提高工作效率。这款小型离心机的相对离心力高达25,001 x g，足以满足所有分子生物学应用需求。所有气密性转子均配套了可以轻松打开和关闭的Eppendorf QuickLock 快速锁定气密性转子盖，离心危险样品更安全。如果 24 孔小型离心机不能满足您的样品通量需求，而您又不需要 Centrifuge 5430 R 冷冻离心机这么全面的离心功能，则可以选择 Centrifuge 5427 R 冷冻离心机。

内有天然制冷剂！

Centrifuge 5427 R是第一个采用碳氢化合物制冷的Eppendorf离心机。碳氢化合物（HC）也称为绿色冷却剂或天然冷却剂。两种最常见的代表性烃类气体为丙烷和乙烷（也分别被称为 R290 和 R170）。 HC冷却液的GWP更直接地等同于 CO₂。 R290是大型商用冷却系统中常用的冷却液。由于其优越的效率和性能，所使用的绿色冷却液的量明显低于使用传统HFC冷却的类似系统。由于这些优点，R290 和R170都在SNAP（美国环保署替代臭氧消耗物质的重大新替代品政策计划）清单上，以供冷藏
实验室仪器使用，如离心机或超低温冰箱。

轻触式盖子闭合，符合人体工程学操作

无需用力拍打盖子或使用不必要的力度 − 只要手指轻轻一按，您就可以离开！

操作简单安全

我们的气密性Eppendorf QuickLock转子只需转动 1/4 圈便可关闭。这不仅节省了您的日常工作时间，还可以减轻腕部压力。

–用于Eppendorf离心管的高速水平转子

使用我们独特的水平转子进行梯度离心或生产紧凑颗粒，适配 24 x 1.5 mL 或 2 mL 离心管，速度高达16,050 x g。搭载气密性QuickLock转子盖，离心危险样品时更快捷安全。

全新明亮的LCD液晶显示屏

我们不仅为广受欢迎的Centrifuge 5427 R冷冻离心机配备了全新显示屏，还添加了一些新功能，让您的日常实验室工作更加轻松——例如一键瞬时离心按钮、运行结束后的计时器以及更精确的<60秒运行时间调节。

您是否拥有Eppendorf产品？已经注册了？

您知道吗，如果您注册了您的Eppendorf产品，您会获得
• 100 epPoints^®（您注册的每个产品都可获得），可用于在我们的奖励商店兑换
• 额外 3 个月的保修期，当您注册离心机、摇床、CO₂ 培养箱或基因扩增仪时
• 每款注册产品均可参加一次Eppendorf 独家抽奖活动（您注册的每个产品都可获得）
• 在 Eppendorf网站上分享您的个人产品故事

操作非常简单！
您可以下载 Eppendorf App，扫描并注册
，或者访问我们的网页，登录您的 myEppendorf 帐户并输入产品序列号。
更多信息： www.eppendorf.com/productregistration

废弃产品

包装处置
离心机包装包含不同材料，包括木制托盘、纸板、塑料防尘罩和一些泡沫部件。材料的回收变得越来越重要：您知道欧洲几乎回收所有的纸板材料吗？我们的离心机包装纸板含有约 50 %的回收材料。请通过收集冰箱纸板包装材料并将其防止在适当的回收容器中，来支持回收有价值原材料的全球可持续发展倡议。对于LD-PE和泡沫制成的塑料防尘罩，我们建议选择可以回收PE材料的专门回收合作伙伴料。建议联系您当地的废物管理公司或设施管理团队，了解适合贵公司的可用回收方案。

仪器处置
我们的离心机可以使用多年。但如果需要更换，我们建议您根据当地要求，妥善处置此类仪器。我们强烈建议您在具有主动制冷仪器方面具有丰富经验的经认证本地回收合作伙伴。在“当地”对其进行处理可减少运输带来的影响，由于冷却液需要以安全、可持续的方式进行移除和回收，建议采用“认证”方式。

去污
该设备用于实验室和/或用于处理生物样品。请记住对需要处理的设备进行充分去污。查看当地要求。有关更多信息，请联系当地的生物安全官员和/或废物管理官员。检查您当地的回收合作伙伴是否有特殊说明和/或文件要求。您也可以使用Eppendorf去污表格作为指导。

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规格	5427 R

ACT 标签	是
最大 RCF	25001 × g
最大 RCF，带固定角转	25001 × g
最大 RCF，带水平转子	16049 × g
速度	100 – 16,220 rpm (50 rpm 步进)
产品类型	离心机
可用转子	9
最大容量	30 × 1.5/2.0 mL
定时器	5 秒至9小时59 分钟
SOFT 软刹车功能	是
噪音水平	< 55 dB(A)，配有转子 FA-45-48-11
电源	230 V, 50 – 60 Hz
最大能耗	495 W
尺寸（长x宽x高）	31.9 × 54.0 × 25.4 cm / 12.6 × 21.3 × 10 in
产品重量	30 kg / 66.1 lb
开盖高度	56 cm / 21.9 in
冷冻型	冷冻型
远程设备监视和通知 (VisioNize^®)	VisioNize^®框已启用
温度范围	-11 °C to +40 °C (This represents the minimum and maximum temperatures that can theoretically be reached. Please note that the actual minimum and maximum temperatures depend on the rotor and speed used.)