What is the meaning of the red knob on the rotor lid?

A fixed-angle rotor with a red knob on the rotor lid indicates aerosol-tightness.

Can the Centrifuge 5430/ 5430 R be opened in case of a power failure?

Yes, in the event of a power failure, the unit can be opened by means of the rotor key. Before the centrifuge is opened, always remove the mains plug from the plug socket and wait for the rotor to come to a standstill. For the emergency release of the centrifuge lid, the small plastic caps on the left-hand and right-hand side of the Centrifuge 5430 have to be removed. Then insert the rotor key into the nut underneath the cap and open the lid latches by turning in anticlockwise direction.

How can the Centrifuge 5430/ 5430 R be cleaned?

The Centrifuge 5430/5430 R can be cleaned with neutral detergents. For disinfection, we recommend alcohol or alcoholic disinfectants. Make sure that the relevant legal requirements and directives are observed when choosing the disinfection method.

Sometimes my Centrifuge 5430/ 5430 R displays "EP". What does that mean?

"EP" is displayed when the automatic standby function is active. It is not an error code. If the centrifuge remains unused for more than 20 min., the standby function is activated, indicated by "EP" on the display. By using the standby key, the centrifuge is re-activated and ready to use. The letters "EP" were chosen because a lot of Eppendorf products already have this prefix in their name.

Does the Centrifuge 5430/ 5430 R recognize an inserted rotor automatically?

The Centrifuge 5430/ 5430 R has got an automatic rotor recognition, so that a newly inserted rotor is recognized automatically as soon as a run is started. The maximum speed will be automatically limited for each rotor. This prevents overspeeding of the rotor.

Which material is the standard fixed-angle rotor for micro test tubes made of?

The aerosol-tight standard fixed-angle rotor is made of massive, anodized aluminum. This material is durable and has got a very good chemical resistance due to the special coating. The aerosol-tight rotor lid is made of the same material.

What tubes can be centrifuged with the Centrifuge 5430/ 5430 R?

Due to the different rotors and adapters, a wide variety of tubes and plates can be centrifuged with the Centrifuge 5430/ 5430 R: • Micro test tubes (0.5 - 2.0 mL) • PCR strips and 0.2 mL PCR tubes • Spin columns • Cryo tubes • Conical tubes (15 mL and 50 mL) • Blood collection tubes • Oak Ridge Tubes (10 mL, 30 mL and 50 mL) • PCR plates (96 and 384 well) • Micro test plates • Deepwell plates (up to 29 mm height) • Slides

Can the Centrifuge 5430/5430 R be operated in the cooling room at 4° C?

The Centrifuge 5430 can be operated at an ambient temperature in the range from 4° C to 40° C. Operation in the cooling room at a temperature of down to 4° C is therefore possible. The permitted range of the ambient temperature is between 10 °C and 40 °C for the Centrifuge 5430 R, so that this centrifuge may not be operated in a cooling room at 4 °C.

What tubes do you recommend for centrifugation at 30,000 x g?

The specifications of the tube manufacturers on the centrifugation resistance must generally be taken into consideration for the centrifugation of tubes of all kinds. This applies in particular for centrifugation at 30,000 x g, as only very few micro test tubes can withstand these extreme gravitational forces to date without breaking. The following tubes can, for example, be centrifuged at 30,000 x g: • Eppendorf Safe-Lock Tubes 0.5 mL • Eppendorf Safe-Lock Tubes 1.5 mL • Eppendorf Tubes 3810X 1.5 mL

When does the opening of the centrifuge lid take place automatically or manually?

Following the end of a centrifuge run, only the lid of the Centrifuge 5430 is automatically opened in order to provide quicker access to the sample and to avoid potential heat accumulation. When working with radioactive material, this function should be deactivated in the device menu and the centrifuge lid manually opened upon conclusion of the centrifugation run. The Centrifuge 5430 R does not come equipped with the option of automatic or manual opening of the centrifuge lid. In order to maintain a consistent temperature in the rotor chamber and in the sample, the centrifuge lid for this device must always be manually opened by pressing the "open" key.

The "Fast Temp" function is a temperature control run of the Centrifuge 5430 R without samples and with a rotor and temperature-specific rotational speed in order to quickly bring the rotor chamber, incl. the rotor, buckets and adapters, to the set nominal temperature. In order that the samples can be centrifuged from the beginning on at the desired temperature, one starts this temperature control run during sample preparation by pressing the "Fast Temp" key. In addition to the current temperature and the g-count (rcf) / rotational speed, the remaining duration of the temperature control run also appears in the display, so that one can more precisely plan the subsequent workflow. You can find a more detailed description in the operating instructions of the Centrifuge 5430/5430 R.

What can I use the "Fast Temp pro" function for?

With the "Fast Temp pro" function it is possible to automatically start the "Fast Temp" temperature control run at a predetermined time. In order that the Centrifuge 5430 R already shows the desired temperature at the start of work, one can set a point in time in the device menu at which the temperature control run should be started. The rotor then begins to spin in the centrifuge at a rotor and temperature-specific rotational speed in order to quickly reach the set nominal temperature. This function can thereby be programmed in such a way that it is carried out once or several times. You can find a more detailed description in the operating instructions of the Centrifuge 5430/5430 R.

When does the continuous cooling of the rotor chamber start?

The continuous cooling starts with each rotor standstill of the Centrifuge 5430 R in order to maintain the preselected nominal temperature of the rotor chamber not only during centrifugation. To this purpose the following prerequisites must be met: - The centrifuge is switched on. - The centrifuge lid is closed. - The nominal temperature is lower than the ambient temperature. - The centrifuge is not in standby mode. Continuous cooling is ended by opening the centrifuge lid or by pressing the standby key.

What is the lowest temperature that can be reached with the Centrifuge 5430 R?

The lowest temperature of the Centrifuge 5430 R is -11 °C. The device is thereby optimized with all rotors in such a way that at least 4 °C is maintained with all rotors at the appropriate maximum speeds.

What is the temperature range of the Centrifuge 5430 R?

With the Centrifuge 5430 R it is possible to set the temperature within a range from -11 °C to +40 °C.

Does the Centrifuge 5430 R has an energy saving function?

Yes, the ECO shut-off automatically switchs off the compressor after 8 hours of non-use. The device has a stand-by button.

For inactivating prions, can the rotors of the Centrifuge 5430/ 5430R be autoclaved at 135 °C ?

The standard rotor FA-45-30-11 can be autoclaved at 135 °C.

What is the benefit of a so-called Kit rotor?

When working with nucleic acid purification kits with spin columns by certain manufacturers, sometimes tube lids cannot be closed any more. This means you have to centrifuge with open tube lids. These tube lids might rip off during the stress of centrifugation and might damage the centrifuge. In order to prevent this, the kit rotor with the raised rotor rim was designed. Open tube lids, supported against the rim, will not rip off during centrifugation. Another feature of this rotor is the enlarged distance between the rotor bores. The enlarged distance between the bores offers a very convenient handling when loading / unloading the rotor. Especially when working with long spin columns (e. g. from Princeton Separations) you will need a larger distance between the bores to load the rotor fully and make use of the complete rotor capacity.

Why are the standard rotors in the micro centrifuges 5418 R, 5420, 5424 R, 5425, 5427 R, 5430 and 5430 R aerosol-tight?

At Eppendorf, the safety of the user always has the highest priority. That is why we now fit our centrifuges with aerosol-tight rotors as standard – at no extra charge.

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Centrifuge 5430/ 5430 R - Fast, versatile, compact

High-speed centrifuges Centrifuge 5430 and 5430 R

Eppendorf Centrifuge 5430 R with 12 centrifuge rotors

Rotary knob control on the Centrifuge 5430

Centrifuge rotor for microplates on Centrifuge 5430

Eppendorf Rotor A-2-MTP for Centrifuge 5430 with lid

Eppendorf centrifuge Rotor F-35-6-30 with 50 mL Conical tubes

Eppendorf centrifuge Rotor F-45-48-11 for Centrifuge 5430 R with lid

Eppendorf centrifuge Rotor FA-45-30-11 for Centrifuge 5430 with lid

Eppendorf Rotor FA-45-24-11-HS for Centrifuge 5430 with lid

Eppendorf Rotor FA-45-24-11-Kit for Centrifuge 5430 with lid

Eppendorf Rotor F-45-64-5-PCR for Centrifuge 5430 with lid

Eppendorf Rotor F-45-18-17-Cryo for Centrifuge 5430 with lid and cryovial adapters

Large backlit display on high-speed centrifuge Centrifuge 5430 R

General Lab Product Version

Product Information

Combine the best of both worlds with Centrifuge 5430 and 5430 R. These high-speed centrifuges have the small footprint of a microcentrifuge and the versatility of a multipurpose centrifuge.

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You will find additional download material at the bottom of this page

Product Information

Technical Data

Features

The 5430 family with the Centrifuge 5430 and 5430 R combines the best features of a microcentrifuge (small footprint) and multipurpose centrifuge (versatility) in one instrument. The high-speed centrifuges offer centrifuge rotors for Eppendorf tubes and PCR strips, but also rotors for microplates and 15 or 50 mL conical tubes.
Additional Eppendorf QuickLock^® centrifuge rotors are now available in the rotor program, including 48-place rotors (1.5 mL or 2.0 mL), a 16-place rotor for Eppendorf Tubes 5.0 mL and a swing-bucket rotor (24 x 1.5 mL or 2.0 mL). With their unmatched versatility, Centrifuge 5430 and Centrifuge 5430 R are the flagship products among the Eppendorf microcentrifuge portfolio.
Available as keypad or knob variant.

Simple and safe handling

Aerosol-tight Eppendorf QuickLock rotors close with just one quarter of a turn. This not only saves you time during everyday use; it also takes strain off your wrist.

The Eppendorf Centrifuge 5430 R: Compact footprint and low access height

The compressor at the back of the Centrifuge 5430 R, the refrigerated version of Centrifuge 5430, is compact and has a low access height.

Advanced operating system of Centrifuge 5430 and 5430 R

The Centrifuge 5430 and 5430 R comes with an advanced operating system, making them more energy efficient due to the smart ECO shut-off and FastTemp pre-cooling functions. They each have a menu-driven, multilingual operation menu with a large backlit display that allows you to save up to 50 user-defined programs and five program keys for easy access to routine programs.
In addition to the standard FastTemp pre-cooling program, Eppendorf high-speed centrifuge Centrifuge 5430 R features the unique software option called FastTemp pro^®. The FastTemp pro function is available for fast automated pre-cooling based on pre-programmable times and dates. FastTemp pro can be set to a specific date or as a repetitive event on specified days of the week. Simply turn the centrifuge into standby mode when you leave your lab and let FastTemp pro take care of pre-cooling in the morning. This not only makes operation easy; it also saves you up to 90 % of energy overnight.
The ECO shut-off function saves you up to 88 % energy overnight by deactivating the compressor after 8 hours of inactivity to extend compressor life. This function can be deactivated in the menu settings.

Proud owner of an Eppendorf product? Already registered?

Did you know that if you register your Eppendorf Centrifuge 5430 with us you will get the following?
• 100 epPoints^® (for each product you register) to redeem in our rewards shop
• Additional 3 months warranty when you register a centrifuge, shaker, CO₂ incubator or cycler
• Entry in an exclusive Eppendorf prize draw (for each product you register)
• Get your personal product story to be posted on the Eppendorf website

Getting started is super easy! Either -
1. Download the Eppendorf App, scan and register OR
2. Visit our webpage, log onto your myEppendorf account and enter the product serial number
More information: www.eppendorf.link/product-registration

End-of-life

Disposal of packaging
The packaging of the Eppendorf Centrifuge 5430 contains different materials, this can include a wooden pallet, cardboard, a plastic dust cover, and some foam parts. Recycling of materials becomes more and more important every day: Are you aware that nearly all cardboard material is recycled in Europe? Our centrifuge packaging cardboard material consists of ca. 50% recycling material. Please support the global sustainability initiative of recycling valuable raw material by also collecting the freezer cardboard packaging material and disposing of it in the appropriate collection container at your organization. In respect to the plastic dust cover made of LD-PE and the foam parts, we recommend to select a dedicated recycling partner where PE material can be recycled. We suggest you contact your local waste hauler or facility management team to understand the available recycling options for your organization.

Disposal of instrument
Our Centrifuge 5430 and 5430 R last for many years, but if they need to be replaced, we kindly ask you to fulfill local requirements for disposal of these instruments. We strongly recommend a certified local recycling partner with experience in instruments with active cooling. Keeping it “local“ reduces the impact of transportation, and the "certified" aspect is recommended due to the safe and sustainable removal and recycling of the cooling liquids if it is a refrigerated centrifuge.

Decontamination
This piece of equipment was used in a laboratory and/ or was used to handle biological samples. Please keep in mind to adequately decontaminate the equipment which needs to be disposed. Check local requirements. For more information, get in contact with your local biosafety officer and/ or waste officer. Check if your local recycling partner has special instructions and/ or documentation requirements. You may also use the Eppendorf decontamination form sheet as guidance.

Read Less

Specifications	5430	5430 R

Max. RCF	30130 × g	30130 × g
Max. RCF with fixed-angle rotor	30130 × g	30130 × g
Max. RCF with swing-bucket rotor	16049 × g	16049 × g
Speed	100 – 17,500 rpm (100 rpm steps)	100 – 17,500 rpm (100 rpm steps)
Product type	Centrifuges	Centrifuges
Rotors available	12	12
Max. capacity	48 × 1.5/2.0 mL, 6 × 50 mL, 2 × MTP	48 × 1.5/2.0 mL, 6 × 50 mL, 2 × MTP
Acceleration time¹⁾	14 s	14 s
Deceleration time¹⁾	15 s	15 s
Timer	30 s to 99:59 h, with continuous run function	30 s to 99:59 h, with continuous run function
SOFT brake function	yes	yes
Noise level	< 58 dB(A) with Rotor F-45-30-11	< 54 dB(A) with Rotor F-45-30-11
Power supply	230 V, 50 – 60 Hz	230 V, 50 – 60 Hz
Max. power consumption	475 W	1.050 W
Dimensions (W × D × H)	34 × 42 × 25 cm / 11.2 × 16.3 × 9.8 in	38 × 64 × 29 cm / 15 × 25.2 × 11.7 in
Product weight	29 kg / 63.9 lb	56 kg / 123.5 lb
Height (with open lid)	56 cm / 21.8 in	63 cm / 24.8 in
Cooling	non-refrigerated	refrigerated
Temperature range	–	-11 °C to +40 °C

1)Acceleration and deceleration times may vary depending on rotor, load and voltage version.

Downloads: Centrifuge 5430/ 5430 R

Brochure

Your Daily Companion (IVD) (IVD)

Eppendorf Microcentrifuges

PDF 8.0MB

English

Selection Guide

Centrifuge Family Overview (IVD)

Overview of all Centrifuges: Capacity and Technical Data

PDF 2.2MB

English

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Centrifuge 5430/ 5430 R - Fast, versatile, compact

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