How does the shaker agitation rate and orbit affect culture growth?

Generally, agitation speed and orbit affect both the aeration and the mixing of the culture. Greater aeration increases oxygen transfer rate (OTR), boosting cell growth. The size of the orbit affects OTR linearly and the speed has a square-law relationship. Therefore, if the size of the orbit doubles, say from 1/2 to 1," the amount of OTR should double〖RPM〗_1/〖RPM〗_2 = (r_2^2)/(r_1^2 ), then 〖RPM〗_2 = √(〖RPM〗_1^2 (r_1/r_2 ) ). If the speed doubles, the OTR should quadruple. Of course, these are general rules and caution should be taken with shear sensitive cells. However, this information can be used to estimate the conditions needed to obtain the same results on different model shakers. For example, to get the same oxygen transfer in a shaker with a 1 in orbit running at 300 rpm, a shaker with 3/4 in orbit must be run at 350 rpm.

Do Eppendorf shakers come with platforms?

Each Eppendorf shaker is available with multiple platform options, which are ordered separately to give you the flexibility to choose the one that best suits your needs. Dedicated platforms include installed clamps of a single size (ie. only 250 mL clamps) and provide in most cases the best capacity but does not allow for the use of different size clamps or holders. Universal Platforms are designed for use with a combination of various-sized clamps and test tube racks providing maximum flexibility. When ordering a Universal Platform, clamps and test tube racks must also be ordered separately.

What is the temperature offset function?

The temperature offset function on many of our shakers allow you to match a reference point" temperature inside the chamber (culture medium) with the "Actual Value" displayed. The temperature offset function on Innova Incubated shakers allows you to monitor the temperature of the media providing you a clearer and more accurate picture. Please be aware that matching reference point temperatures to the Actual means you have to deal with many variables such as: greater evaporation; heat gains generated by cultures growing - (slowly at first and much more during their growth phase) and heat generated by the friction of media with the glass at high speeds or use of baffled flasks. Over time, the reference point temperature offset may need to change due to these varying conditions.

I want to grow my cultures at 27°C, do I need an incubated shaker with refrigeration?

The Eppendorf Incubated, non-refrigerated Shakers Innova and I-Series are designed to maintain temperatures of ambient plus 5 °C to its maximum operating temperature. The Eppendorf Incubated Excella shakers will operate from ambient plus 7 °C to maximum temperature. It is critical to understand that "ambient temperature is not the temperature on the wall thermometer (room temperature), but the temperature measured one meter from the front of the machine.

Why are some flask clamps supplied with an extra spring set?

This "extra spring set" (we call it a girdle) is included with clamps for 2 L, 2.8 L, 4 L and 6 L flasks. It is intended to be used with the already installed spring set. This girdle helps prevent large flasks from spinning inside the clamp and reduces the risk of flasks becoming dislodged or scored by the steel clamp. One girdle is already in place on the clamp, the other is packed separately. To install this extra “girdle”, follow the instructions in the manual supplied with the shaker.

How is temperature accuracy defined by Eppendorf?

Accuracy is defined as the degree of closeness between the displayed value and the actual value as determined by a traceable device. All Eppendorf Incubated Innova and I-Series shakers have a temperature Accuracy of ±0.1 °C from 30 – 40 °C, ±0.5 °C over the remaining range. New Brunswick Incubated Excella Shakers are accurate within 0.1°C at 37°C.

How is Uniformity defined by Eppendorf?

Uniformity is the ability to maintain uniform temepratures throughout the platform. New Brunswick Incubated Innova Shaker temperatures will not deviate by more than +/- 0.25 °C at 37 °C.

The orbit of a shaker is the distance traveled around the central drive. This can be descibed in inches, centimeters, or milimeters. If our shakers had a one inch (2.54 cm) orbit. That means that that the lateral movement of the platform would equal one inch from one point to another that is 180° opposite that position. All of the New Brunswick. shakers have a circular orbit

What wavelengths are used in your growth lamps in a photosynthetic light bank?

Each of the bulbs that is fit into our photobanks is a wide spectrum bulb that provides the red and blue wavelength of energy needed for photosynthesis. Innova 42R has six bulbs as the Innova 43R and 44R have nine bulbs. To have this option installed, the shaker needs to have the refrigeration option installed as well.

Which screws will fit to which platform ?

All of Eppendorf Inc. clamps and accessories are packaged with multiple screws to fix them to our universal platforms, included in M1013-0261 clamp screw kit (Stainless steel platform screw #S2117-3050 (10-32 x 5/16 in (7.9mm)), Stainless Steel, Aluminum, and Aluminum and Phenolic (Plastic) #S2116-3051 (10-24 x 5/16 in (7.9 mm)), and Wood #S2116-3101 (10-24 x 5/8 in (15.87mm)).

How can I disinfect, clean, maintain my shaker?

We recommend cleaning the exterior of our units with a damp cloth or any standard household or laboratory cleaner to wipe the surfaces clean. Do not use abrasive or corrosive compounds to clean the instruments as they may damage the unit and void the warranty. It is the responsibility of theuser to carry out the proper decontamination procedures if hazardous materials are spilled on or inside the equipment. Before using any cleaning or decontamination method other than those suggested by the manufacturer, users should contact Eppendorf Inc. to ensure that the proposed method will not damage the equipment. Commercially available household bleach solutions, when diluted at a 1:10 ratio, are effective in routine decontamination of the instrument. The method for decontaminating a spill depends on the nature of the spill. Spills involving fresh cultures or samples of known concentrations of biomass should be flooded with decontamination solution and soaked for at least 5 minutes before cleanup. Spills involving samples with high concentrations of biomass, or involving organic matter, or occuring in areeas warmer than ambient/room temperature, should be exposed to decontamination solution for at least one hour before being cleaned up. Personnel involved in the cleanup of any spill should wear gloves, safety glasses, and a laboratory coat or gown during the cleanup process. Respiratory protection should be considered for spills where aerosolization is suspected.

Which flask type fits in which clamp ?

Please see the Accessories for New Brunswick™ Shakers: Dimensions and Capacities in the Userguides section of the shaker product page.

Which vessel fits in Eppendorf Test tube racks ?

Please see the Accessories for New Brunswick™ Shakers: Dimensions and Capacities in the Userguides section of the shaker product page.

Is it possible to monitor relative humidity in the Innova 42, 43, 44? ?

Innova 42, 43, 44 can be equipped with a relative humidity sensor optionally (Humidity monitor). This option allows monitoring the actual humidity level in the chamber and indicates the value on the screen. The option does not allow humidity control. The humidity sensor can be retrofitted by Eppendorf Technical Service. If humidity monitoring is installed, the maximum temperature is 60 °C. The maximum reachable relative humidity is around 40 – 45 % rH (at 37 °C) by filling the spill pan.

Why is the triple eccentric drive special ? What is the difference between Innova and Excella Shakers drive ?

The Innova and I-series Triple eccentric drive consists of three individual bearings and eccentric shafts mounted in a triangular fashion for precise uniform circular motion at every point on the platform. The heavy duty drive has a single shaft that utilizes springs to aid in balancing the load. This drive works great with small to medium loads at lower speeds.

How can I shake MTPs ? Which parameters ?

Eppendorf shakers offer a variety of Micro Titer Plate holders that attach to our univeral platforms. Our shakers offer a variety of orbits up to 5.1 cm (2 in) and speeds up to 500 rpm. If mixing is what is needed at higher speeds and smaller orbits you can find Eppendorf Mixers.

How do I use of sticky pads/sticky tape?

Eppendorf Sticky Pads are recommended for use on our sticky pad platform or our sticky pad adapter kit (M1250-9504). On the S44i universal platform sticky pad can be used directly. The adapter kit is easily fixed to our universal platforms. Any size flask can be used with the sticky pad with a 1 in (2.54 cm) orbit up to 250 rpm, any size flask can be used with the 2 in (5.1 cm) orbit up to 200 rpm. To clean the sticky pad, just wash with soap and water. this will revive" the stickiness, but the sticky pad must be completely dry before replacing on the platform.

What is the heavy load platform ?

The Heavy Duty Platform (M1282-9928) is designed for use in the Innova 44 only. This platform is 0.95 cm thick alluminum plate that is recommended for shaking full loads of flasks greater than 1 L at or above 20 % fill volumes or flasks with baffles.

What is the orbit of a shaker?

An orbital shaker moves the platform in a circular shaking motion. The term orbit describes the diameter of this circular movement. Typical orbit sizes are 1.9 cm, 2.5 and 5 cm.

What orbit shall I take?

An orbital shaker is used to grow microorganisms or cells growing in suspension, like bacteria, yeast, fungal, insect, algae or plant cells. Orbital shaking enables a homogeneous distribution and aeration of the cultures by increasing the oxygen transfer rate (OTR). There are different influencing parameters on shaking performance, e.g. the shaker orbit, the applied speed, the vessel shape, the liquid viscosity and the filling volume. The 2.5 mm orbit is the standard solution which works well for most applications. For some applications, e.g. large vessels > 2 Liter, especially large volume Fernbach flasks or very shear sensitive cells, a larger shaking diameter with the consequence of a reduced speed can be beneficial.

I use two shakers with different orbits. Can I transfer my experimental conditions to a shaker with a different orbit?

Yes, the rpm can be adapted with help of the following formula .

What platform types are offered with the shakers?

Universal platform and a variety of dedicated platforms are available. University platforms offer maximum flexibility having a hole-pattern that allows using virtually any combination of clamps and other accessories. Dedicated platforms come complete with pre-installed clamps of one size. The hole-pattern of the dedicated platform is optimized for the use of one specific size of clamps. It allows in most cased more capacity but can only be used with that size of clamp. Please note that the platform has to be ordered in addition to the shaker.

为什么要定期维护轨道摇床？

对于有可移动部件的所有产品，磨损总是难以避免的，并且可能长时间影响产品操作一致性。 Eppendorf 产品在设计上可以最大程度减轻磨损，然而定期维护可以在产品性能超出可接受容差范围之外前尽早发现和追踪过度磨损。定期维护也可能是用户的一项监管要求。

轨道摇床需要多久维护一次？

Eppendorf 建议每 12 个月进行一次维护。然而，可能与用户的监管要求存在较大差异，后者可能要求进行更频繁的维护。

轨道摇床维护服务价格？

费用取决于产品型号和所需执行的维护水平。请联系您当地的 Eppendorf 客服获取个体化报价。 www.eppendorf.com/contact

IQ 或 OQ 是什么？有什么用？

安装和操作验证；安装验证记录产品已按照制造商规范和指南正确交付和安装。操作验证检查设备是否正常工作，并且符合制造商规范和指南（因此，只能由设备制造商提供该服务）。 IQ/OQ 主要适用于实施质量管理流程的机构，但也适用于所有用户。

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New Brunswick Innova^® 42/42R - 可叠放生物摇床

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您是否正在寻找一款具有Innova可靠性的多功能可叠放生物摇床？ Innova 42/42R是我们受欢迎的摇床产品之一，因为它易于清洁、提供多种选件（如静置培养搁板、防止琼脂板蒸发的培养抽屉、紫外线杀菌或光合灯）并且可灵活放置在实验室各处。

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Innova 42 和 42R（带制冷）摇床是我们配置和附件多样化的生物摇床。可以将两台摇床叠放在一起，也可以放置在标准高度实验台上方或下方。无论在什么样的场景下，都可以使用侧开门方便地从箱体取放样品。可选配固定培养抽屉、通气管、远程报警、湿度检测和承液盘。此外，42R 可选配光照组件和紫外消毒灯。

铸铁结构的 New Brunswick 三偏心轴平衡驱动专为高转速、高承载–应用设计，这种设计已经过超过 70 年的考验，今天仍然服务着全世界的科研人员。通过用户界面可以方便地对速度、温度、时间以及光照组件（如已安装）进行编程。可以通过添加 VisioNize box 来增强监测以及其他通知功能。可以原位进行定期维护，尽可能缩短停机时间。

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规格	Innova^® 42，轨道直径 1.9 cm（3/4 英寸）	Innova^® 42R，轨道直径 1.9 cm（3/4 英寸）	Innova^® 42，轨道直径 2.5 cm（1 英寸）	Innova^® 42R，轨道直径 2.5 cm（1 英寸）

外部深度（盖/门打开）	131  cm (51.6 in)	131  cm (51.6 in)	131  cm (51.6 in)	131  cm (51.6 in)
定时器	0.1–99.9 小时，可连续运行	0.1–99.9 小时，可连续运行	0.1–99.9 小时，可连续运行	0.1–99.9 小时，可连续运行
电源	230 V ± 10 %, 50 Hz	230 V ± 10 %, 50 Hz	230 V ± 10 %, 50 Hz	230 V ± 10 %, 50 Hz
尺寸（长x宽x高）	63.5 × 74.5 × 81.8 cm / 25 × 29.3 × 32.8 in	63.5 × 74.5 × 81.8 cm / 25 × 29.3 × 32.8 in	63.5 × 74.5 × 81.8 cm / 25 × 29.3 × 32.8 in	63.5 × 74.5 × 81.8 cm / 25 × 29.3 × 32.8 in
产品重量	98.5 kg / 217 lb	121 kg / 267 lb	98.5 kg / 217 lb	121 kg / 267 lb
温度范围	环境温度+10 °C至80 °C	环境温度以下20 °C至80 °C	环境温度+10 °C至80 °C	环境温度以下20 °C至80 °C
声光报警	是	是	是	是
温度均一性	±0.25°C，37°C 时 °C	±0.25°C，37°C 时 °C	±0.25°C，37°C 时 °C	±0.25°C，37°C 时 °C
可用程序模式	• 持续转速和温度控制 • 多步骤编程控制 • RS-232 通讯端口	• 持续转速和温度控制 • 多步骤编程控制 • RS-232 通讯端口	• 持续转速和温度控制 • 多步骤编程控制 • RS-232 通讯端口	• 持续转速和温度控制 • 多步骤编程控制 • RS-232 通讯端口
驱动装置	三偏心轴平衡驱动	三偏心轴平衡驱动	三偏心轴平衡驱动	三偏心轴平衡驱动
台上 (B)，台下 (U)，落地 (F) 或叠放 (S)	B, U, S (x2)	B, U, S (x2)	B, U, S (x2)	B, U, S (x2)
通气管	是	是	是	是
水/湿度监测	是	是	是	是
箱体内电插座	是	是	是	是
内部便捷灯	是	是	是	是
存储卡	断电后重启自动运行记录参数设置	断电后重启自动运行记录参数设置	断电后重启自动运行记录参数设置	断电后重启自动运行记录参数设置
马达类型	固态无碳刷马达	固态无碳刷马达	固态无碳刷马达	固态无碳刷马达
光照组件照明	否	是	否	是
摇床板尺寸	46 × 46 cm (18 × 18 in)	46 × 46 cm (18 × 18 in)	46 × 46 cm (18 × 18 in)	46 × 46 cm (18 × 18 in)
冷冻型	−	是	−	是
滑出式摇床板	否	否	否	否
正在打开门	向前方打开	向前方打开	向前方打开	向前方打开
速度范围	25 – 400 rpm；叠放或使用 >500 mL 锥形瓶，25 – 300	25 – 400 rpm；叠放或使用 >500 mL 锥形瓶，25 – 300	25 – 400 rpm；叠放或使用 >500 mL 锥形瓶，25 – 300	25 – 400 rpm；叠放或使用 >500 mL 锥形瓶，25 – 300
多步骤编程	是	是	是	是
每个程序的步骤	15	15	15	15
存储的程序	4	4	4	4
cETLus 和 CE 标志	是	是	是	是
容量	中型	中型	中型	中型
编程步骤/可存储程序	15/4	15/4	15/4	15/4
远程设备监视和通知 (VisioNize^®)	VisioNize^®框已启用	VisioNize^®框已启用	VisioNize^®框已启用	VisioNize^®框已启用
温度范围	环境温度以上10 °C至80 °C	环境温度以下20 °C至80 °C	环境温度以上10 °C至80 °C	环境温度以下20 °C至80 °C

搅拌
轨道	1.9 cm (0.75″)	1.9 cm (0.75″)	2.5 cm (1″)	2.5 cm (1″)