How do I determine the volume values when calibrating gravimetrically?

The measuring values of the analysis scales are multiplied by the correction factor Z (see SOP pipettes). This takes into account the density of the water at the relevant temperature and the relevant air.

What does EN ISO 8655 cover?

ISO 8655 addresses the needs of suppliers, test houses, and users and specifies the general requirements for piston-operated liquid handling devices. It is structured in seven chapters: ISO 8655-1: Terminology, general requirements, and user recommendations ISO 8655-2: Piston pipettes ISO 8655-3: Piston burettes ISO 8655-4: Dilutors ISO 8655-5: Dispensers ISO 8655-6: Gravimetric method for the determination of measurement error ISO 8655-7: Non-gravimetric methods for assessment of equipment performance (titration, photometry)

What do the terms random error and systematic error mean in a pipette calibration?

The systematic error describes the deviation of the dispensed volume from the nominal volume or from the selected volume of the piston stroke device, and is determined by establishing the mean value of (usually) 10 measurements. The random error describes the mean variation of the dispensed volumes from the mean value of the dispensed volumes, and is determined by calculating the repeat standard deviation of (usually) 10 measurements.

Why are the Z factors listed in our SOP different from factors which can be derived from density reference values (of water)?

The Z factor is included in EN ISO 8655-6 and was incorporated into our SOP. As we understand it, it was incorporated in the standard by the Physikalisch Technische Bundesanstalt in Braunschweig. The Z factor describes the density of water at a particular temperature using a 5th degree polynomial. Furthermore, the density of water is corrected by the density of air (derived from air pressure) and a fixed correction value for balances. These corrections are the key reasons for the deviation from density values in various books of tables.

How can the Multipette/Repeater M4 be calibrated?

To calibrate Multipettes/Repeaters in accordance with EN ISO 8655, three different volumes with ten measured values each have to be checked. These three volumes are the nominal volume (= the maximum volume to be dispensed), 50% of the nominal volume and 10% of the nominal volume of the Combitips advanced used. The checking procedure, the test conditions, and the limits in accordance with EN ISO 8655 are described in our "Eppendorf SOP for checking pipettes". The SOP is available on our web page.

Can the M4 Multipette/Repeater M4 be autoclaved?

No, the Multipette/Repeater M4 cannot be autoclaved. We recommend wiping the device with a cloth that has been moistened with a disinfectant (e.g. 60% isopropanol, 12% bleach). Please ensure that no moisture can get into the device during cleaning.

Can Combitips advanced advanced be partially filled as well?

Yes, with the Multipette/Repeater M4, Combitips advanced can be partially filled as well. To ensure accurate dosing, it is mandatory that the pistons of the Combitips advanced are moved to a defined position after aspiration. Discard the steps by pressing the operating lever until the display stops flashing and “steps 0” is displayed. If the selection dial position is below 4, several steps must be discarded.

Why can the Multipette/Repeater M4 only be operated with Combitips advanced?

When using a positive displacement system, the dispenser and the dispenser tip must be perfectly matched. This will ensure that the high demands with regard to precision and accuracy are reliably met. Combitips advanced are perfectly matched to Eppendorf Multipettes and, therefore, to the Multipette/Repeater M4. In combination with the center sleeve of the Multipette M4, accurate dosing for wall dispensing is supported in particular.

What is the maximum number of dispensing steps that can be carried out with the Multipette/Repeater M4?

In position 0.5, 100 dispensing steps can be performed with the Multipette/repeater M4.

What is the volume range covered by the Multipette/Repeater M4 when Combitips advanced are used?

Eppendorf offers 9 different sizes of Combitips advanced. This enables the Multipette/Repeater M4 to cover a dispensing volume of 1 µL - 10 mL.

Which chemicals can be dispensed with the Multipette/Repeater M4?

During the development of the Multipette/Repeater M4, the use of materials with a high chemical resistance was a priority. Of course, the chemical resistance of the Combitips advanced is also relevant here. You can find a detailed list of the chemical resistance for both products in the corresponding operating manual or in the instructions for use.

Can you dispense 2 µL in a free jet?

With the Combitip advanced 0.1 mL in selector dial position 1 and the Combitip advanced 0.2 mL in selector dial position 0.5, a volume of 2 µL can be dispensed in a free jet. All volumes can be dispensed in a free jet, if handled correctly. If you want to carry out all dispensing steps as a free jet, the reverse stroke should be dispensed in a free jet as well. Please note that the technical data document recommends wall dispensing.

How can I extend the life of the battery as much as possible?

The life of the battery very much depends on the type and frequency of use. To extend the life of the battery as much as possible, the Multipette M4 goes into sleep mode when it is not in use. You should also remove the Combitips advanced immediately after use and not store the Multipette M4 with the Combitip advanced inserted to extend the life of the battery.

How long will the battery last (for the use of the display) after the battery symbol appears?

If the battery symbol only shows with one segment, the battery capacity is very low. A few more dosing series can be carried out. Have a replacement battery to hand. As soon as the empty battery symbol appears, the battery should be replaced.

Does the Multipette/Repeater M4 also work without a battery?

Yes. The Multipette/Repeater M4 also works without a battery. The precision of the dosing is due to the reliable mechanics of the Multipette/Repeater M4. It will not be affected if the battery fails. To help with the selection of the correct dispensing volume, a volume table is included in the short instructions and in the operating manual. In addition, the Combitips advanced indicate the dispensing volume in selector dial position 1. This volume can be multiplied with the selector dial position to calculate the set dispensing volume.

How much does the Multipette/Repeater M4 weigh?

The Multipette/Repeater M4 only weighs 105 g.

What does the “PhysioCare concept” symbol on the Multipette/Repeater M4 mean?

The symbol means that the Multipette/Repeater M4 has been developed in accordance with the considerations of the Eppendorf PhysioCare concept. With its three areas, the concept includes the whole range of ergonomics, not just a single element. Instead of focusing on a particular product feature, the products with the PhysioCare symbol provide a holistic solution. Further details on the PhysioCare Concept ® are available at http://physiocare.eppendorf.com.

Does a specific dispensing angle need to be observed during dispensing?

Yes. If the Multipette/Repeater M4 is held at an angle that is too flat (< 45°C), dosing accuracy is affected. Hold the device as upright as possible, but in a position that is comfortable for you. Even when dispensing the last step, the Multipette/Repeater M4 should be held as straight as possible.

What does “steps” on the display mean?

The Multipette/Repeater M4 has a step counter. If a Combitip advanced is used alongside “steps” (here: dispensing steps), the number of dispensing steps that can be carried out with a full Combitip advanced is displayed. During dispensing, the number of dispensing steps that have already been carried out are displayed. When the selector dial position is adjusted, the number of dispensing steps that can still be carried out is displayed.

Can the step counter also count the dispensing steps if the Combitips advanced are only partially filled?

Yes, the step counter also counts the dispensing steps that were carried out when the Combitip advanced is only partially filled once. As with a full Combitip, the reverse stroke must be carried out first (display flashes). Please note with this type of filling that the step counter continues to count beyond the residual stroke lock.

Can the step counter also count the dispensing steps if the Combitip advanced is filled several times?

Yes, the step counter also counts the dispensing steps when the Combitip advanced is filled to capacity several times.

How are the Combitips advanced ejected?

To eject the Combitips advanced, push the lower filling lever down as far as it will go (dispensing the remaining volume). Then the Combitip advanced can be ejected without touching it, by pressing the upper operating lever.

Is it possible to attach the Multipette/Repeater M4 to a wall?

Yes. The Multipette/Repeater M4 comes with a holder which can be inserted in a pipette carousel or it can be fixed to a clean glass or ceramic surface using the enclosed adhesive strip.

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Multipette^® M4

Side view of the Multipette® M4 multi-dispenser pipette from Eppendorf

The range of Combitips® advanced dispenser tips offer a wide spectrum of possibilities

Multipette® M4 multi-dispenser with an assortment of fitting Combitips® advanced positive displacement pipette tips

Store your Multipette® M4 multi-dispenser securely on a Pipette Carousel 2 or Charger Carousel 2 by Eppendorf

Pipette Holder 2 for Multipette® M4 multi-dispenser pipette from Eppendorf

Multipette® M4 Multi-Dispenser Held By Scientist (Product Close-Up)

Female and Male Scientists Working with Multipette® M4 and Eppendorf Conical Tubes

ACT label of sustainability certification for Multipette® M4 starter kit

产品信息

彻底告别耗时费力的连续分液工作！EppendorfMultipette M4分液器无需反复多次抽吸液体，十分适合步骤较多的连续分液任务。搭配Combitips^® advanced分液吸头， Multipette便可轻松处理传统分液器难以移取的粘性、挥发性或发泡液体。Multipette/Combitips正位移分液系统采用密封吸头，可确保在处理传染性、毒性或放射性液体时高度安全。

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用途

特征

仅需一次吸液就能进行多达 100 次的分液操作
Multipette M4手动外置活塞式分液器仅需一次吸液就能进行多达 100 次的分液操作，使其成为长时间重复移液任务的理想精密仪器，如试管或工作板加样和等分试剂。液体通过外置活塞吸入和分配，这意味着没有可能受液体样品特性影响的气体活塞。这可确保始终分配正确的体积——无论液体的密度、粘度和挥发性如何。
Combitips^® advanced外置活塞式移液器自动识别吸头，省去了耗时的体积计算，并且采用密封设计，保护用户、仪器和样品安全。处理感染性、有毒性或放射性液体的理想选择。

1978年Eppendorf发布了Multipette/Combitips分液系统，此后对其进行了多次改进。这是移液系统，仅需一次吸液就能进行多次分液操作。这一概念仍然是高效液体处理的驱动力，也是许多使用工作板或系列样品管的实验室的必备工具。

此款连续移液器配备先进操作界面和传感器控制器，操作便捷

步骤计数器
• 显示已执行完成的分液步骤数

体积选择刻度盘
• Combitips advanced共有20种不同体积设置，适用于不同尺寸

运动传感器
即使Combitips advanced分液吸头没有弹出，Multipette M4也会在不使用时自动关机以节省电力。当再次移动时，它会自动启动，并且“记住”执行的分液步骤数，以提高效率。

Combitips advanced装卸传感器。
• 自动Combitips advanced吸头识别和体积显示
• 确保Combitips advanced和Multipette M4之间的安全连接

让耗时费力的连续移液工作变得更加轻松舒适

Multipette M4配备易于操作和易于调节的体积选择刻度盘，有助轻松管理连续移液工作。采用符合人体工程学的扶手设计，带来放松的手部姿势并且易于操作，确保Multipette M4为全天工作提供更高水平的舒适度。

符合人体工程学的操作杆既可以装填和分配液体，也可以单手操作脱卸Combitips advanced分液吸头。

如何使用Multipette M4连续移液器？

Multipette M4的操作使用十分简单：只需将适配的分液吸头（Combitips advanced或ViscoTip）插在M4上，同时按下操作杆即可。移液器会自动识别吸头大小，接着您便可以直接选择满足您应用需求的分液体积和步骤。不同分液步骤的体积设置可以更改。将吸头浸入液体样品中，轻轻按压操作杆，吸取需要分配的总体积。再次按压操作杆，启动反向排液，以最大限度提高准确度。然后缓慢推动操作杆，直至无法推动为止，即可开始向目标容器中分配样品。如需了解更多信息，请参阅《快速入门指南》。

为您的分液器和移液器提供方便的存储选项
我们的Multipette M4壁式移液器支架或Eppendorf Pipette Holder System可以粘贴到几乎任何表面，或与我们的充电或非充电手动移液器旋转式支架整合。或者，一台Multipette M4分液器可以通过Eppendorf存储在Pipette Stand 2中。

处置生命周期结束后的包装和分液器

Multipette M4分液器包装含有纸板、塑料薄膜等不同材料。我们的 Multipette包装用纸板含有约 92 %的回收材料。请收集包装材料并分类放于贵司废物收集容器，以实际行动支持回收有价值原材料的全球可持续性倡议。对于采用LD-PE制成的包装薄膜，我们建议选择一位专门回收聚乙烯材料的合作伙伴。

Eppendorf液体处理仪器的设计使用年限长达数年。如果需要处置Multipette，请注意它们包含电子元件和电池并可能已被污染。有关电子和实验室废物的回收要求和更好的实践，请联系当地生物安全官员和/或废物管理部门。如有文件要求，您可以将《Eppendorf消毒证书》作为指导。

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规格	Multipette^® M4 启动套装 - 4982000314	Multipette^® M4 - 4982000012

ACT 标签	是	是
产品类型	分液器	分液器
移液类型	外置活塞系统	外置活塞系统
通道数量	1-通道	1-通道
体积范围	1 µL – 10 mL	1 µL – 10 mL
操作模式	手动	手动
随机测量误差	see Combitips advanced	see Combitips advanced
系统测量误差	see Combitips advanced	see Combitips advanced
高温高压灭菌	否	否
电池类型	纽扣电池	纽扣电池
Consumables	Combitips^® advanced, ViscoTip^®	Combitips^® advanced, ViscoTip^®
开放系统（第三方耗材）	否	否
交付内容	多功能分液器，电池（已插入），操作手册，证书，Combitips^® advanced Box 2.0 吸头，Combitips^® advanced 混合装（每种尺寸吸头各1个），Holder 2 移液器支架	多功能分液器，电池（已插入），操作手册，证书，Combitips advanced 2.5 mL吸头，Holder 2 移液器支架