Cleaning/Disinfection: How should I clean/disinfect my CellXpert incubator?

The recommended disinfecting agent for use with the CellXpert incubator is a solution of 70% isopropanol (isopropyl alcohol) or ethanol and 30% sterile distilled water. Carefully follow the cleaning guidelines described in your user’s manual. If you have any further questions regarding the cleaning and disinfection procedure please contact your local distributor: www.eppendorf.com/contact .

High temperature disinfection (HTD): what is the maximum temperature that is achieved during the HTD cycle of CellXpert incubators?

The maximum temperature of the HTD cycle is 180°C (CellXpert C170i) and 140°C (CellXpert C170) respectively.

Data Export: Can I export performance data from CellXpert incubators?

The CellXpert C170i is equipped with two USB ports on the front panel for quick and easy data export. In addition, you can remotely monitor the status of your CO 2 incubator within the VisioNize ® lab system.

Direct Heating Advantages: I am hesitant to move from a water-jacketed incubator to a direct heat system. Why should I switch?

While water-jacketed (WJ) incubators have, in the past, been suitable for generalized cell culture, they truly are an outdated technology. WJ incubators are bulky, often taking up valuable lab space, offer less capacity in relation to that space, have lower performance and control, are difficult to maintain and do little to prevent contamination. High-temperature disinfection is also not possible in WJ designs. The direct-heat design provides an innovative solution to these issues and is ideal for culture in today’s laboratory.

Maintenance: Are there any parts which should be regularly renewed on my CellXpert?

The filter in the gas supply line should be replaced once per year.

Temperature Control: What is the temperature range for your CO2 Incubators?

The temperature on Eppendorf direct-heat incubators is 4 °C above ambient temperature to 50 °C.

Which safety measures do you recommend regarding the handling of CO 2 and N 2 in the laboratory?

When CO 2 and/or N 2 concentration in the air is too high there is a risk of suffocation. Performing a risk assessment is strongly recommended, independent of the existing national guidelines, if incubators are operated with gases. We recommend implementing measures against the identified dangers, for example: - Measures that issue an alert when critical gas concentration is reached in the laboratory (gas detectors) - Measures that ensure air exchange (ventilation systems) both during normal operation and when a critical gas concentration is reached in the laboratory

Can the door hinge of the CellXpert C170 and C170i be changed from left to right or vice versa?

Yes, the door hinge can be changed directly in your laboratory.

Why should I have my incubator serviced at regular intervals?

Eppendorf Performance Plans offer a selection of preventive maintenance services which ensure a consistent performance of the device and confidence in the results.

How often does my incubator need to be serviced?

This depends on how intensively it is used and varies depending on the load on the device. However, we recommend to have it serviced at least once a year.

What do you need to look out for before carrying out a service?

The device needs to be decontaminated first. The device must be freely accessible and all samples removed from the device. You should also ensure that the device has cooled down already.

What types of service programs are available for incubators?

The available service programs can be found under the respective instrument in the Service & Support / Maintenance and Certification Services section.

How much are the different incubator service programs?

The cost depends on the model and on the service that is performed. Please contact your local Eppendorf Service. www.eppendorf.com/contact

Please contact your local Eppendorf Service Team. You find the contact data on our homepage or in the information materials. www.eppendorf.com/contact

What is IQ or OQ and what do I need them for?

Installation and operational qualification; the IQ documents that the device has been delivered and installed correctly. The OQ checks the correct functioning of a device. IQ/OQ ensure that your quality management requirements are met and that your device operates properly and in accordance with the manufacturer’s specifications.

How often does the OQ need to be carried out for an incubator?

The company is responsible for technical checks of devices. Eppendorf recommends regular device qualifications depending on the intensity of use, however, they should be carried out at least once a year.

How can my incubator be cleaned/ disinfected?

Use a solution of 70% isopropanol (isopropyl alcohol) and 30% distilled water as a disinfectant. Follow the cleaning guidelines carefully that are described in your user manual.

Which parts of the incubator should be replaced at regular intervals?

Replace the HEPA filter of the CO 2 supply line and the HEPA filter with automatic zero setting * at least once a year. (* Auto-zero filter is only used in R-models.)

What types of training or online training do you offer to enable me to improve my skills?

Please visit the Eppendorf webpage about the Eppendorf Training Center or contact your local Eppendorf Service for further information and a quotation. www.eppendorf.com/training

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CellXpert^® C170 - Cell Culture Incubator

Cell culture incubator CellXpert® C170 from Eppendorf in front view

Stacked cell culture incubators CellXpert®

Lab technician with cell culture incubator CellXpert® filled with cell culture plates and flasks

Graph showing fast gas and temperature recovery < 5 min without setpoint overshoot

Graph - Costs for N2 and CO2 in cell culture incubator CellXpert® C170i for hypoxic conditions in 5 years

Product Information

The C170 is the basic version of the C170i. It comes with the same uniform atmosphere, fast recovery, cleanability, high usable space but for example with a simpler user interface and less in-field upgradeable options. Use the flyer “C170i vs. C170” to find the right device for your needs.

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Products (2)

Services (11)

Accessories (12)

VisioNize (6)

2 Products

Cell culture incubator CellXpert<sup>®</sup> C170 from Eppendorf in front view

CellXpert^® C170

non-segmented inner door, handle right side
100 – 127 V/50 – 60 Hz (US/JP/South America), 3 shelves included

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Catalog no. 6734010015

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$ 13,238.00

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CellXpert^® C170

inner door with 4 door segments, handle right side
100 – 127 V/50 – 60 Hz (US/JP/South America), 4 shelves included

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Catalog no. 6734010045

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$ 15,673.00

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Technical Data

Features

CellXpert – a new family of Eppendorf CO₂ incubators

CellXpert^® C170i or C170 cell culture incubator – What is the best choice for your needs?

The C170 is the more economical, basic version of the C170i. If you need, e.g. more future flexibility, O₂ control, copper interior, humidity monitoring, advanced documentation, user management, or if you have a requirement for 180 °C disinfection – the C170i is recommended.

Download guide: Find the right CellXpert for your needs

Contamination prevention – If it's easy to do, it's more likely to get done

Prevention of lab downtime and sample loss is essential for you to deliver cell culture results in time? You cannot afford to spend too much time and money on standardized cleaning of your cell culture incubator or on changing costly, non-sterilizable, fan-associated HEPA-filters? The CellXpert C170 cell culture incubator supports you with:

• Integrated 140 °C high temperature disinfection
• Minimal internal parts can be removed in 40 s and wiped quickly
• Nowhere to hide for contaminants and cleaned fast: Smooth, seamless incubator chamber
• No fan or non-sterilizable fan-associated HEPA-filters that can become potential contamination sources
• Option for 4-segmented inner door for added protection

Learn more: Video – Easy cleaning and reliable contamination prevention with CellXpert CO2 incubators

Optimized growth conditions for reproducible cell culture results

Many cell culture experiments are not reproducible due to several reasons. A key factor in missing reproducibility can be atmospheric variations inside the CO₂ incubator (e.g. due to extended/frequent door openings or heterogeneous atmosphere distribution). CellXpert^® cell culture incubators support more reproducible culturing results by:

• Temperature uniformity throughout the incubator chamber, verified at 27 spots inside based on German Norm DIN 12880
• Fast CO₂ and temperature recovery after door opening < 5 min without setpoint overshoot
• Higher protection from vibrations and turbulence on the top shelf with fanless incubator design – put your vessels wherever you like
• State-of-the-art dual channel and high temperature resistant IR-sensor for CO₂ control
• Dedicated IQ/OQ and Eppendorf service contracts

Is a fan inside your cell culture incubator necessary for fast atmosphere recovery after door opening and spatial homogeneity?

Fanless cell culture incubators have always been a solid choice when easy cleaning, effective contamination prevention, and protection from vibrations and air turbulences is key. In addition, these incubators usually provide significantly more usable space because of less internal parts compared to fan-assisted cell culture incubators (e.g. no ducts, filters, fan itself etc.). In the past, however, fast atmosphere recovery after door opening and spatial homogeneity compared to fan-assisted cell culture incubators have always been a challenge for fanless CO₂incubators. CellXpert^® cell culture incubators solved this former disadvantage by various technologies like multiple, independent temperature sensors (see section below), as well as advanced microprocessor and gas control.

The result: CellXpert^® cell culture incubators can easily compete with fan-assisted devices regarding recovery and spatial homogeneity of CO₂ and temperature (see below) – while providing easy cleaning, reliable contamination prevention, and high usable space.

No fan = No costly, fan-associated internal HEPA-filters

Because of their fanless design, CellXpert CO₂ incubators don´t need fan-associated internal HEPA-filters.

Generally, fan-associated HEPA-filters are included in heat-sensitive cartridges and need to be removed before starting a high temperature disinfection. Afterwards, the HEPA-filter (that traps particles, but does not destroy them) often needs to be reinstalled at the backside of the incubator chamber. This results in a high contamination risk due to extended door opening and reaching inside the clean incubator chamber a full arm's length.

CellXpert cell culture incubators reduce significant recurring costs for fan-associated HEPA-filters that must be replaced regularly (usually twice yearly is recommended) – while offering strong contamination protection.

Innovative CO₂ incubator heating concept

High temperature uniformity is required to compare cell growth in vessels at different locations inside the cell culture incubator. In the CellXpert, this uniformity is achieved by advanced microprocessor control and an innovative heating concept. This concept replaces the use of one or two temperature sensors (the second is almost always just used to control for harmful overtemperature) with multiple, independent sensors in different incubator locations. The power of this novel approach has been verified by measuring the temperature deviation among 27 spots inside the cell culture incubator – a measurement that is based on the German norm DIN 12880.

What is the best place for your vessels inside the cell culture incubator?
Learn more: White Paper about CO₂ incubator homogeneity

Do you put cell culture vessels on the top shelf of your fan-assisted cell culture incubator? Are you afraid of vibrations or increased medium evaporation?

Have you ever experienced variations between cells grown in vessels on different incubator shelves, especially between the top shelf and others?

In addition to atmospheric inhomogeneities, cells on the top shelf can experience vibrations and increased medium evaporation that can be induced by incubator fans. Fan-induced vibrations can lead to vessel vibration and thus uneven cell growth – especially in the case of sensitive cells. Additionally, fan-induced air turbulences can disrupt the protective micro-atmosphere above the medium and thus lead to increase medium evaporation. Vibrations and fan-induced air turbulences can be effectively avoided with fanless cell culture incubators like the CellXpert.
Put your vessels wherever you like and enjoy peace of mind using the top shelf – for more reproducible results and more valuable space.

Save money and cell culture lab space

In addition to initial incubator acquisition costs, several running costs add to the overall lab expenses and total costs of ownership. These factors include, e.g. regular replacement of fan-associated HEPA-filters or UV-lamps, loss of lab space (low vessel capacity vs. footprint ratio), or high gas consumption including more frequent cylinder exchange efforts.
CellXpert^® cell culture incubators significantly reduce lab costs by:

• Saving lab space: Up to 25 % more usable space inside within a small footprint (no additional interior parts like fan, air ducts, HEPA-filters etc.)
• Saving up to 1000 € / 1100 USD in five years: No internal expendable parts like fan-associated HEPA-filters or UV-lamps
• Significantly lower CO₂ consumption by smart gas control and low clearance construction
• Long-lasting quality – Made in Germany
• Features and accessories already included: Three shelves, dual-channel IR-sensor for CO₂ control (instead of TC-sensor) etc.

Learn more: White Paper - How to Save Money with CellXpert CO2 incubators

Efficient lab and device management with VisioNize^® Lab Suite

Are you interested in monitoring your lab devices and receiving notifications e.g., for device alarms? A digital, connected lab offers even more.

Gain access to a modular range of digital services and choose what works for your lab. VisioNize Lab Suite offers everything you need as a Lab Manager or Scientist to get started to digitally manage your lab for increased sample security, compliance needs and maintenance management.

Chart your own course to digital lab excellence! Find out more

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Specifications	CellXpert^® C170

Capacity	170 L (6.0 ft³)
Power supply	100 – 127 V, 50 – 60 Hz
Dimensions (W × D × H)	71.8 × 71.5 × 90.0 cm / 28.3 × 28.1 × 35.4 in
Product weight	113 kg / 249 lb
High temperature disinfection (HTD)	140 °C
Sealed inner glass doors	yes
Segmented door options	4 segments
Temperature control increment	±0.1 °C
Temperature stability at 37 °C	±0.1 °C
Temperature range	Ambient +4 °C to 50 °C
Temperature uniformity	±0.3 °C
CO₂ range	0.1 – 20 %
CO₂ control increment	±0.1 %
CO₂ stability at 5 % CO₂	±0.1 %
CO₂ uniformity	±0.1 %
Number of shelves (standard/max)	3 (4 with 4-segmented door)/8
Max. load/shelf	10 kg
Max. load/rack	40 kg
Data export interface	Ethernet
Benchtop (B), Under Bench (U), Floor (F) or Stackable (S)	B, U, F, S (x2)
Copper package	–
Access port	2
On-board data logging	–
Water/Humidity monitoring	–
BMS relay	yes
Temperature range	Ambient +4 °C to 50 °C
Temperature accuracy	±0.4 °C