What is the meaning of the red knob on the rotor lid?

A fixed-angle rotor with a red knob on the rotor lid indicates aerosol-tightness.

Can the Centrifuge 5427 R be opened in the event of a power failure?

Yes, in the event of a power failure, the unit can be opened by means of the rotor key. Before the centrifuge is opened, always remove the mains plug from the plug socket and wait for the rotor to come to a standstill. For the emergency release of the centrifuge lid, the small plastic cap on the right front corner of the 5427 R has to be removed. Then insert the rotor key into the nut underneath the cap and open the lid latches by turning in anticlockwise direction.

How can the Centrifuge 5427 R be cleaned?

The Centrifuge 5427 R can be cleaned with neutral detergents. For disinfection, we recommend alcohol or alcoholic disinfectants. Make sure that the relevant legal requirements and directives are observed when choosing the disinfection method.

Sometimes my Centrifuge 5427 R displays "EP". What does that mean?"

EP" is displayed when the automatic standby function is active. If the centrifuge remains unused for more than 15 min., the standby function is activated, indicated by "EP" on the display. By using any key, the centrifuge is re-activated and ready to use.

Can the Centrifuge 5427 R automatically recognize an installed rotor?

The Centrifuge 5427 R has an automatic rotor recognition system. A newly installed rotor is recognized at the beginning of the run. The maximum speed of each rotor is automatically limited, if necessary, to prevent over-speeding of the rotors.

What benefits do I gain from the centrifugation of my samples with the swing bucket rotor?

Swing-bucket rotors are particularly advantageous for gradient centrifugation applications as horizontal layers are formed that remain in this position at the end of centrifugation (e.g. blood/Ficoll).

What is the benefit of a so-called Kit rotor?

When working with nucleic acid purification kits with spin columns by certain manufacturers, sometimes tube lids cannot be closed any more. This means you have to centrifuge with open tube lids. These tube lids might rip off during the stress of centrifugation and might damage the centrifuge. In order to prevent this, the kit rotor with the raised rotor rim was designed. Open tube lids, supported against the rim, will not rip off during centrifugation. Another feature of this rotor is the enlarged distance between the rotor bores. The enlarged distance between the bores offers a very convenient handling when loading / unloading the rotor. Especially when working with long spin columns (e. g. from Princeton Separations) you will need a larger distance between the bores to load the rotor fully and make use of the complete rotor capacity.

What tubes can be centrifuged with the Centrifuge 5427 R?

Due to the different rotors and adapters, a wide variety of tubes can be centrifuged with the Centrifuge 5427 R: • Micro test tubes (0.5 - 5 mL) • Cryo-Vials • HPLC-Vials • PCR strips and 0.2 mL PCR tubes • Spin columns (1.5/2.0 mL) • Microtainer (0.6 mL)

In which rotors can I centrifuge 0.2 mL PCR tubes?

Up to 48 0.2 mL PCR tubes (or 6 x 8-tube PCR strips) can be centrifuged in the PCR rotor F-45-48-5-PCR. Furthermore, 0.2 mL PCR tubes can be used in each rotor for 1.5/ 2.0 mL micro test tubes using an adapter

In which rotors can I centrifuge 0.5 mL micro test tubes?

0.5 mL micro test tubes can be centrifuged in all rotors for 1.5/ 2.0 mL micro test tubes using an adapter.

In which rotors can I centrifuge 5 mL micro test tubes?

Up to 16x 5 mL micro test tubes can be centrifuged in the aerosol-tight rotor FA-45-12-17.

In which rotor can I centrifuge spin columns?

Spin columns or small filtration columns, for example for centrifugation of DNA or RNA, can be used in all rotors for 1.5/2.0 mL micro test tubes. In particular, however, the kit rotor F-45-24-11-Kit is specially suitable for such spin columns, as open tube lids are supported by the raised edge of this rotor type and are no longer torn off (e.g. during elution of the sample from the column into a micro test tube)

The "Fast Temp" function is a temperature control run of the Centrifuge 5427 R without samples and with a rotor and temperature-specific rotational speed in order to quickly bring the rotor chamber, incl. the rotor, buckets and adapters, to the set nominal temperature. In order that the samples can be centrifuged from the beginning on at the desired temperature, one starts this temperature control run during sample preparation by pressing the "Fast Temp" key. The display shows the current temperature and the g-force (rcf) / rotational speed (rpm) as well as "FT" as symbol for a "Fast Temp" temperature run. You can find a more detailed description in the operating instructions of the Centrifuge 5427 R.

What is the temperature range of the Centrifuge 5427 R?

With the Centrifuge 5427 R it is possible to set the temperature within a range from -11 °C to +40 °C.

Does the Centrifuge 5427 R has an energy saving function?

Yes, with "ECO shut-off" the continuous cooling is switched off if the centrifuge is not used for longer than the preset time. With the default settings, the continuous cooling ends after 8h. In the menu setting "TEMP" the continuous cooling can be limited to 1h, 2h or 4h.

For what applications are aerosol-tight rotors important?

Aerosol-tight rotors are always recommended or even prescribed when working with hazardous or potentially hazardous substances. These can, for example, be viruses, pathogenic bacteria, potentially infectious blood samples or radioactive sample material.

Why are the standard rotors in the micro centrifuges 5418 R, 5420, 5424 R, 5425, 5427 R, 5430 and 5430 R aerosol-tight?

At Eppendorf, the safety of the user always has the highest priority. That is why we now fit our centrifuges with aerosol-tight rotors as standard – at no extra charge.

What kind of cooling liquid is used for the Centrifuge 5427 R?

The cooling system operates with 75g of the "green" cooling liquid R290, also known as propane.

What is the SOFT function?

The SOFT function of the Centrifuge 5427 R allows slower and gentler acceleration and braking compared to the normal acceleration and braking times. This setting is especially suited for applications like e.g. density gradient centrifugation where remixing of phases must be prevented.

Are there any additional safety features implemented due to R290?

Like all our cooling systems, the cooling system of Centrifuge 5427R is a closed system which has no leakage of cooling liquid. For the unlikely case of leakage two additional safety measures are implemeted: All potentially sparks emitting components are shielded, before the centrifuge is ready for operation, a ventilation system takes care to exchange the air in the centrifuge. Therefore, you might notice a few more seconds prolonged initialization phase.

Do I have to take special cautions during transport, storage or installation of the Centrifuge 5427 R due to the green cooling liquid?

No special transport requirements are necessary as only 75 g propane are used. This also means that the centrifuge can be transported by air freight. The room where the centrifuge 5427 R is stored and installed needs to have a volume of minimal 10 m³. In case that more than one device is installed, the requirement stays to 10 m³. During operation leave a safety clearance of 50 cm around the device and at least 30 cm to the rear of the device.

What is the temperature range of the Centrifuge 5427 R?

With the Centrifuge 5427 R it is possible to set the temperature within a range from -11 °C to +40 °C.

How do I recognize aerosol-tight rotors?

Firstly, the aerosol-tight rotors can be recognized by their designation abbreviation “FA” or “AT”; secondly, Eppendorf has color-coded the aerosol-tight rotors. All aerosol-tight rotors have a red rotor lid screw, and a red ring is printed on to the rotor shaft.

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Centrifuge 5427 R - For high-throughput

Centrifuge 5427 R with hydrocarbon cooling

High-throughput microcentrifuge for up to 48 x 1.5/2 mL tubes

Woman closing microcentrifuge lid with one finger touch

Centrifuge 5427 R offers a great versatility with nine different rotor options

Microcentrifuge rotor for up to 48 x 1.5/2 mL microtubes

Microcentrifuge rotor for up to 30 x 1.5/2 mL microtubes

Microcentrifuge swing-bucket rotor for 24 x 1.5 mL tubes

Microcentrifuge rotor for 24 x 1.5/2 mL spin-colum tubes

Microcentrifuge rotor for 24 x 1.5/2 mL tubes

Microcentrifuge rotor for 5 mL Eppendorf tubes

ACT certificate for sustainability score of Eppendorf Centrifuge 5427 R

ACT certificate for sustainability score of Eppendorf Centrifuge 5427 R with rotor FA45-30-11

ACT certificate for sustainability score of Eppendorf Centrifuge 5427 R with rotor FA45-48-11

ACT certificate for sustainability score of Eppendorf Centrifuge 5427 R without rotor

Product Information

Eppendorf Centrifuge 5427 R is a high-speed, refrigerated microcentrifuge for high-throughput research. It doubles your tube capacity compared to a standard 24-place microcentrifuge thanks to its 48-place double-row rotor, while contributing to a more sustainable lab environment with its natural refrigerant.

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1 Products

Centrifuge 5427 R

rotary knobs, refrigerated, without rotor
ACT, 230 V/50 – 60 Hz (GB)

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Catalog no. 5429000065

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Product Information

Technical Data

Features

Introducing our refrigerated microcentrifuge with natural refrigerant: the Eppendorf Centrifuge 5427 R

Global warming and its consequences are one of the greatest challenges of our time. Hydrofluorocarbons, which have been used as refrigerants in cooling systems such as air-conditioners as well as laboratory centrifuges or freezers in the past, can contribute to global warming due to their chemical structure. To protect our planet for future generations, it is important to switch to much more environmentally friendly, "green" cooling agents − hydrocarbons. These natural coolants have a Global Warming Potential similar to CO₂. To support this matter in the laboratory environment, Eppendorf now offers the first centrifuge in our portfolio with a natural refrigerant to protect your samples − and the planet.
The refrigerated microcentrifuge Centrifuge 5427 R was designed with high-throughput research applications in mind. With its aerosol-tight 48-place rotor it handles even the toughest challenges in a safe and reliable manner to provide you the efficiency boost you need. With speeds of up to 25,001 x g, this microcentrifuge is fast enough for all your molecular biology applications. All aerosol-tight rotors are equipped with the Eppendorf QuickLock lids for easy rotor lid opening and closing to enable safe centrifugation of hazardous samples. Choose the Centrifuge 5427 R if a 24-place microcentrifuge doesn't meet your needs regarding tube capacity and if you don't need the extra versatility our cross-over Centrifuge 5430 R can offer you.

Natural refrigerant inside!

Centrifuge 5427 R is the first Eppendorf centrifuge with hydrocarbon cooling. Hydrocarbons (HC) are also known as green or natural coolants. The two most commonly used representatives are propane and ethane (known as R290 and R170, respectively). The GWP of HC cooling liquids are more directly equivalent to CO₂. R290 is a commonly used cooling liquid in large commercial cooling systems. Due to their superior efficiency and performance, the amount of green cooling liquids used is significantly lower than that of a similar system with classic cooling by HFCs. Due to these advantages, R290 and R170 are both on the SNAP (Significant New Alternatives Policy Program for substitution of ozone-depleting substances by EPA) list for approved coolants for usage in refrigerated
laboratory instruments such as centrifuges or even ULT freezers.

Soft-touch lid closure for ergonomic operation

No need to smash the lid or use unnecessary force − just a soft finger push and you are good to go!

Simple and safe handling

Our aerosol-tight Eppendorf QuickLock rotors close with just one quarter of a turn. This not only saves you time during everyday use, it also takes strain off your wrist.

High–speed swing-bucket rotor for Eppendorf tubes

Perform gradient centrifugation or produce compact pellets with our unique swing–bucket rotor for 24 x 1.5 mL or 2 mL tubes with speeds up to 16,050 x g. Centrifuge hazardous samples safely and effortlessly thanks to the aerosol-tight QuickLock rotor lid.

New, brighly lit LCD

We did not only add a new display to our popular Centrifuge 5427 R but also new features to make your everyday lab life easier − like the one-push short spin button, the end-of run timer and an even finer time adjustment for runs <60 seconds.

Proud owner of an Eppendorf product? Already registered?

Did you know that if you register your Eppendorf Centrifuge 5427 R with us you will get the following?
• 100 epPoints^® (for each product you register) to redeem in our rewards shop
• Additional 3 months warranty when you register a centrifuge, shaker, CO₂ incubator or cycler
• Entry in an exclusive Eppendorf prize draw (for each product you register)
• Get your personal product story to be posted on the Eppendorf website

Getting started is super easy! Either -
1. Download the Eppendorf App, scan and register OR
2. Visit our webpage, log onto your myEppendorf account and enter the product serial number
More information: www.eppendorf.link/product-registration

End-of-life

Disposal of packaging
The packaging of the Eppendorf Centrifuge 5427 R contains different materials, this can include a wooden pallet, cardboard, a plastic dust cover, and some foam parts. Recycling of materials becomes more and more important every day: Are you aware that nearly all cardboard material is recycled in Europe? Our centrifuge packaging cardboard material consists of ca. 50% recycling material. Please support the global sustainability initiative of recycling valuable raw material by also collecting the freezer cardboard packaging material and disposing of it in the appropriate collection container at your organization. In respect to the plastic dust cover made of LD-PE and the foam parts, we recommend to select a dedicated recycling partner where PE material can be recycled. We suggest you contact your local waste hauler or facility management team to understand the available recycling options for your organization.

Disposal of instrument
Our Eppendorf Centrifuge 5427 R lasts for many years, but if it needs to be replaced, we kindly ask you to fulfill local requirements for disposal of these instruments. We strongly recommend a certified local recycling partner with experience in instruments with active cooling. Keeping it “local“ reduces the impact of transportation, and the "certified" aspect is recommended due to the safe and sustainable removal and recycling of the cooling liquids if it is a refrigerated centrifuge.

Decontamination
This piece of equipment was used in a laboratory and/ or was used to handle biological samples. Please keep in mind to adequately decontaminate the equipment which needs to be disposed. Check local requirements. For more information, get in contact with your local biosafety officer and/ or waste officer. Check if your local recycling partner has special instructions and/ or documentation requirements. You may also use the Eppendorf decontamination form sheet as guidance.

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Specifications	Centrifuge 5427 R

ACT label	yes
Max. RCF	25001 × g
Max. RCF with fixed-angle rotor	25001 × g
Max. RCF with swing-bucket rotor	16049 × g
Speed	100 – 16,220 rpm (50 rpm steps)
Product type	Centrifuges
Rotors available	9
Max. capacity	48 × 1.5/2.0 mL
Timer	5 s to 9:59 h
SOFT brake function	yes
Noise level	< 55 dB(A) with rotor FA-45-48-11
Power supply	230 V, 50 – 60 Hz
Max. power consumption	495 W
Dimensions (W × D × H)	31.9 × 54.0 × 25.4 cm / 12.6 × 21.3 × 10 in
Product weight	30 kg / 66.1 lb
Height (with open lid)	56 cm / 21.9 in
Cooling	refrigerated
Remote Device Monitoring and Notifications (VisioNize^®)	VisioNize^® box enabled
Temperature range	-11 °C to +40 °C (This represents the minimum and maximum temperatures that can theoretically be reached. Please note that the actual minimum and maximum temperatures depend on the rotor and speed used.)

Downloads: Centrifuge 5427 R

Selection Guide

Centrifuge Family Overview (IVD)

Overview of all Centrifuges: Capacity and Technical Data

PDF 2.2MB

English

Brochure

Protect Your Samples – And the Planet (IVD)

Centrifuge 5427 R with hydrocarbon cooling

PDF .6MB

English

Brochure

Your Daily Companion (IVD) (IVD)

Eppendorf Microcentrifuges

PDF 8.0MB

English

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Centrifuge 5427 R - For high-throughput