How many parameters can I control using a DASGIP or DASbox system?

DASGIP Parallel Bioreactor Systems provide comprehensive concurrent monitoring and control of process parameters. During the process run, events are logged together with up to 70 online process variables per bioreactor. In addition to the various DASGIP modules for monitoring and control, with DASware analyze users can integrate analytical devices provided by third parties. Seamless integration into the DASGIP or DASbox system even allows for closed loop solutions such as automated feeding strategies based on the current glucose concentration. Each process value can be used as a trigger enabling users to automate their process.

How many parameters can be displayed in a process overview in DASware control?

DASware control users can freely select all relevant process variables of all or selected bioreactors to be displayed in one chart. There are no limitations in the number of process parameters. When using DASware analyze, the displayed process parameters even include integrated data from third party laboratory devices, such as nutrient analyzers, mass spectrometers or Raman. The software features tables and trend graphs of all measured values. It continuously logs and stores all data and events. These data are immediately accessible for evaluation using e.g. Microsoft Excel. Advanced charting functionalities ease further data analysis and comparison of runs.

Do I need programming skills to automate my process in DASware control?

DASware control includes a set of easy to implement automation features without any need of programming. Profiles and triggers allow for various control strategies, all of them easy to select by mouse click with parameters entered manually or just copy-pasted. Users with programming skills and special control requirements, however, are free to run scripted control strategies and profiles as well.

Can I change triggers, profiles, cascades and scripts online in DASware control?

Set-points, profiles, cascades, and scripts are online editable in DASware control. Offline values and events can be entered during or after the run and documented with a time stamp. These time stamps can be set individually, e.g to meet the time, when the sample was taken. This provides users with great flexibility to interact with the control system and reconsider parameter settings even in highly automated processes. All events, including online changes, are recorded in a SQL Server database.

How difficult is it to modify a DASGIP system subsequently?

Due to the unique modular design of the DASGIP Parallel Bioreactor System upgrades and modifications can easily be done: Functions like OD monitoring, exhaust analysis or extra feed lines can be added as modules to the control system. It is just as easy to substitute the bioreactor type or to upgrade a 4-vessel system to eight vessels. Actually, system modifications are that simple, some customers even share specific modules. For instance, the DASGIP GA4 exhaust analyzer is renowned for being moved from one bioreactor system to another. Like this, it delivers reliable in-depth analysis of OTR, CTR, and RQ for many bioreactors on demand.

How many speed-controlled pumps do the DASGIP bioreactors feature?

Up to eight peristaltic speed-controlled pumps per vessel offer continuous flow rates from 0.3 mL/h - 5 L/h. Each pump can be operated independently. Two different pump head sizes for the multi pump modules DASGIP MP4 and MP8, fitting various tube sizes make the DASGIP multi pumps a flexible and accurate solution.

How are DASGIP vessels agitated?

DASGIP bioreactors feature powerful overhead drives with agitation speeds ranging 30 – 1,600 rpm. The DASGIP TC4SC4 controls temperature and agitation in up to four bioreactors. Users can individually define each outlet set-point as well as choose from different operation modes (e.g. continuous or interval agitation).

Can I integrate DASGIP with my existing historians & supervisory control?

Yes, with the software DASware connect an optional server allows corporate-wide exchange of all process data via seamless integration with historians and supervisory control systems such as Delta V, OSI Pi, etc. This includes events, set-up information, and system status.

How can I harness data generated by the DASGIP system?

With DASware control all data and events are logged in one central SQL Server database. Further features such as export into Excel, PDF, and other file formats via one mouse click and strong charting capabilities ease plotting and data analysis. To harness data from numerous projects, on the long-run advanced archiving capabilities and data retrieval is required. DASware discover offers comprehensive and user-defined archiving functions and data retrieval for powerful data comparison and analysis. Users can, for example, link the utilized strain or cell line with its media composition, the controller setpoints and feeding profiles as well as with achieved product yields or viable cell densities to select for the best optimized combination and process.

Does DASware control give any alarms in case of critical deviation?

Critical deviation of process parameters such as pH, DO, redox, and temperature are color-coded in the software and saved in the event log. DASware control professional additionally allows notification of supervisory control systems or mobile communication tools via SMS or e-mail.

Can you help adjusting my controller loop parameters in DASware control?

DASGIP controllers are PI controllers, which are configured with default controller parameters adapted to the type of vessel and default experimental requirements. Depending on special customer requirements it might be necessary to modify these parameters. Our service will be happy to help you finding the right settings.

How to calibrate a pH sensor in DASware control?

DASware control software allows for parallel calibration of all sensors used within one system with all data logged in the central database together with set-points, profiles, cascades, process values, user interactions and other events. Good maintenance will prolong the sensors' lifetime whereas high temperatures (i.e. during autoclavation), alkaline solutions (i.e. during alkaline deactivation), repeated etchings and improper maintenance will reduce the electrode's lifetime.

How reliable are exhaust data when working at benchtop scale?

Exhaust oxygen and carbon dioxide concentrations of multiple bioreactor units are continuously monitored with the DASGIP GA exhaust analyzer. Together with the mass flow measurement and the input values of oxygen and carbon dioxide concentrations, automatic calculation of oxygen transfer rate (OTR), carbon dioxide transfer rate (CTR), and respiratory quotient (RQ) is possible, permitting direct conclusions on the metabolic state of a culture. As the DASGIP GA considers humidity, volume, and total flow typical sources for inaccurate OTR and CTR data in small working volumes are eliminated.

Is it possible to automatically control the foam in DASGIP vessels?

All DASGIP vessels can be equipped with foam control components. All you need is a level/foam sensor connected to the DASGIP sensor module (e.g. PH4PO4L) and a feed line for dosing of the antifoam agent. Now foam formation can be detected by the level sensor and antifoam agent is automatically pumped into the vessel until all foam is collapsed. The DASware control software allows any feed line to be used for antifoam or level control. Since medium composition has a strong impact on the electrical conductivity of medium and foam, every conductivity-based level sensor needs to be adjusted to foam and medium conductivity. DASware control supports monitoring conductivity and free adjustment of the conductivity threshold.

What maintenance is required for Eppendorf bioprocess systems?

As all complex technical systems Eppendorf bioprocess equipment should be maintained regularly to keep all parts in good condition. This maintenance avoids cost-intensive down times and contributes to preservation of value. A complete preventive maintenance is recommended once a year. Additional maintenance actions are recommended to be executed by the user prior to every run or in regular cycles (e.g. every month). Our service will be happy to advice.

What do I have to consider when returning equipment for repair?

Equipment returned to Eppendorf for service or any other reason needs to be decontaminated first. Please fill out the Decontamination Form that you will find in the “Service & Support” section of the Eppendorf website and attach it to the package. Equipment which was sent without the completely filled form cannot be handled. This form must also be completed before any on-site service, whether covered under warranty or not, can commence.

What does your warranty cover?

Eppendorf issues a warranty of one year from the day of delivery on its products in accordance with our terms and conditions. Excluded are consumables such as cables, tubing, glassware, and sensors as well as used material.

Does Eppendorf offer bioreactor training?

We highly recommend a training at the customer’s site for all new customers. Besides this initial training, Eppendorf also offers individual trainings adapted to the user’s requirements like training of new employees or advanced training/recapitulation for employees already having experience with DASGIP or DASbox systems. All trainings are performed at the customer’s site using the customer’s equipment to have the training focused on the customer’s demands. The structure and methods of the training can be chosen to the customer’s requirements from general overview to detailed sessions on specific products or issues. Training in small groups is as possible as personal guidance.

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DASGIP^® Parallel Bioreactor System

Product Information

The DASGIP Parallel Bioreactor System is a bioreactor system for research and process development in working volumes from 200 mL to 1.8 L. It can operate up to 16 glass or BioBLU^® Single-Use Bioreactors in parallel and is suitable for microbial fermentation and cell culture, including the cultivation of stem cells. The modular design allows users to choose the most suitable configuration for their application needs.

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Product Information

Technical Data

Applications

Features

The DASGIP Parallel Bioreactor System enables highly efficient process development by allowing precise control of critical process parameters, rapid comparison of numerous experimental conditions, and optimal utilization of all generated data.
• Parallel operation of up to 16 bioreactors saves time and space and improves reproducibility.
• Easily convertible from single-use to reusable bioreactors and vice versa.
• DASware^® software suite enables precise monitoring and control of critical process parameters, as well as the implementation of tailored feedback loops, feeding strategies and other features.
• Seamless integration with the Bioprocess Autosampler allows for automated sampling 24/7 to gain complete datasets.
• Connects to BioNsight^® cloud software for remote process monitoring and simple, remote analysis of all data sets to make informed decisions.

Operate multiple bioreactors in parallel

• The DASGIP Parallel Bioreactor System is designed as a 4-fold system that is expandable to up to 16 parallel operating bioreactors.
• The setpoints, control strategies, and inoculation time of each bioreactor can be controlled individually from one software installation. Like this, multiple experimental conditions can be tested in parallel.

Automate bioprocess sampling

• The bioreactor system can be seamlessly integrated with the Bioprocess Autosampler, which takes samples from multiple bioreactors 24/7 in short and regular intervals and stores them tempered for later analysis.
• The Bioprocess Autosampler can be installed with new as well as retrofitted to existing bioreactor control systems.

Suitable for cell culture and microbial fermentation

• The DASGIP Parallel Bioreactor System suits the needs of different cell types, including bacteria, yeasts, insect cells, differentiated mammalian cells, and stem cells.
• Two variants are tailored to support the gas flow requirements of microbial and cell culture applications, respectively.
• The DASGIP Parallel Bioreactor System is compatible with bioreactors designed to suit the needs of microbial and cell cultures, respectively.

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Specifications	DASGIP^® System for Cell Culture	DASGIP^® System for Microbiology

Application	Cell culture	Microbiology
Number of parallel units	Up to 16	Up to 16
Software	DASware control, other DASware optional	DASware control, other DASware optional
User Interface	Process computer with monitor	Process computer with monitor
Typical power consumption (4-fold system without process computer)	480 W (at 230 V)/ 432 W (at 115 V) (595 W/552 W without DASGIP Bioblock)	541 W (at 230 V)/ 467 W (at 115 V) (639 W/584 W without DASGIP Bioblock)
Dimensions (W × D × H, 4-fold system without process computer)	140 x 70 x 53 cm (55 x 28 x 21 in)	140 x 70 x 53 cm (55 x 28 x 21 in)
Typical weight	78 kg (60 kg without DASGIP Bioblock)	96 kg (78 kg without DASGIP Bioblock)
Bacteria/Yeast/Fungi	yes	yes
Mammalian/Animal	yes	yes
Insect	yes	yes
Interchangeable Vessels	yes	yes
Validatable	no	no
Stem cells	yes	yes

Bioreactors
Vessels	Glass/single-use vessels	Glass/single-use vessels
Sterilization	Autoclavable/ pre-sterilized	Autoclavable/ pre-sterilized
Total volume	1.5 L to 2.3 L	1.3 L to 2.1 L

Agitation
Drive	Direct/magnetic overhead drive	Direct/magnetic overhead drive
Speed ranges	30 – 1,250 rpm (standard)/ 100 – 1,600 rpm (optional)	100 – 1,600 rpm (standard)/ 30 – 1,250 rpm (optional)
Impellers	Pitched-blade	Rushton-type

Gassing
Gas supply	TMFC; overlay and/or sparger	TMFC/Rotameter; sparger
Standard gas flow rates	0.1 – 50 sL/h, 0.1 – 40 sL/h CO₂ (DASGIP MX4/4, other modules available)	0.5 – 250 sL/h, 0.5 – 150 sL/h CO₂ (DASGIP MX4/4H, other modules available)
Standard gas mixing	Air, N₂, O₂ and/or CO₂	Air, N₂, O₂ and/or CO₂

Feeding
Feed lines per vessel	Up to 8	Up to 8
Standard feed rates (depending on tube diameter)	0.3 – 9.5 mL/h to 13 – 420 mL/h (DASGIP MP8) 10 – 70 mL/h to 0.4 – 5 L/h (DASGIP MP4)	0.3 – 9.5 mL/h to 13 – 420 mL/h (DASGIP MP8) 10 – 70 mL/h to 0.4 – 5 L/h (DASGIP MP4)

Monitoring and Control
Temperature control	Heating and cooling integrated in DASGIP Bioblock (additional cooling fingers optional)	Heating and cooling integrated in DASGIP Bioblock (additional cooling fingers optional)
Standard temperature range (at 25° C room temp.)	5 K above cooling agent temperature – 99°C (DASGIP Bioblock)¹⁾	5 K above cooling agent temperature – 99°C (DASGIP Bioblock)¹⁾
pH control	−	Acid and/or base, and other set-ups
DO control	Cascade (O₂ concentration, gas flow rate), and other set-ups	Cascade (agitation speed, optional O₂ concentration, optional gas flow rate), and other set-ups
ORP (redox) measurement	-	Optional
Level/foam	Optional	Optional
OD measurement	Optional (DASGIP OD4)	Optional (DASGIP OD4)
Exhaust condensation	Water-cooled or liquid-free (Peltier with DASGIP EGC4)	Water-cooled or liquid-free (Peltier with DASGIP EGC4)
Exhaust analysis	Optional (DASGIP GA4)	Optional (DASGIP GA4)
1)Application-specific operating conditions apply.

Downloads: DASGIP^® Parallel Bioreactor System

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DASGIP® Parallel Bioreactor System