The BioBLU Single-Use Bioreactors for cell culture applications at a glance:
BioBLU c Single-Use Bioreactors: For the scalable cultivation of mammalian and insect cells in suspension, on microcarriers, or as cell aggregates. BioBLU c Single-Use Bioreactors are available in different sizes for 400-fold scalability.
• BioBLU 0.3c (working volume 100 – 250 mL)
• BioBLU 1c (working volume 320 mL – 1.25 L)
• BioBLU 3c (working volume 1.25 – 3.75 L)
• BioBLU 10c (working volume 3.3 L – 10 L)
• BioBLU 50c (working volume 18 – 40 L)
BioBLU 0.3sc Single-Use Bioreactor: Optimized to support the special needs of stem cells, such as low-shear mixing and aggregate formation. Working volume 100 – 250 mL.
BioBLU 5p Single-Use Bioreactor: For the cultivation of adherent cells and for perfusion processes in a fixed working volume of 3.75 L. This packed-bed bioreactor is preloaded with Fibra-Cel® Disks, a solid growth support matrix.
The BioBLU Single-Use Bioreactors are compatible with the small and bench-scale bioprocess controllers from Eppendorf and selected third-party controllers.
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BioBLU® c Single-Use Bioreactors
BioBLU c Single-Use Bioreactors Group 0.3c, 0.3sc,1c, 3c, 5p, 10c, 50c, cropped
BioBLU 5p with medium, front view
Product Information
BioBLU c Single-Use Bioreactors are rigid-walled stirred-tank bioreactors for the cultivation of human and animal cells. The scalable BioBLU c portfolio enables for a 400-fold cell expansion (working volume range of 100 mL – 40 L). Different BioBLU Single-Use Bioreactor versions have been developed for the specific needs of suspension-, anchorage-dependent-, and stem cells.BioBLU 0.3c
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20 Products
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Product Information
Applications
Features
Product Information
Bioreactor body and head plate
• Dimensions: Standard bioreactor dimensions and impeller size of the BioBLU c Single-Use Bioreactors allow efficient mixing and mass transfer, and scalable results.
• Equipment: BioBLU Single-Use Bioreactors are assembled with sparger, overlay, gas filters for inlet and exhaust as well as penetrations for pH, DO, and temperature sensors, liquid additions, sampling, and harvest. All head plate ports are clearly marked for ease of use. Spare Pg 13.5 ports can be equipped with single-use septa, tri-ports and compression fitting adaptors for more flexibility.
• Tubing: Weldable and easy to connect tubing offer in-field flexibility and ease of use.
• Motor: Sealed magnetic drive with fully enclosed bearings maintains vessel sterility.
• Exhaust Treatment: Effective liquid-free exhaust condensation (Peltier) or electric heater band.
• Sterilization: BioBLU Single-Use Bioreactors are delivered sterile. Irradiated by > 15 kGy (β) or > 25 kGy (X-ray). SAL-level 10-6 for X-ray irradiated BioBLU® Single-Use Bioreactors.
Impellers designed for cell culture applications
• All BioBLU c bioreactor sizes are available with pitched-blade impellers for cell culture applications.
• BioBLU 0.3sc bioreactors are equipped with an 8-blade impeller optimized for reduced cell settling and very good mixing already at low agitation speeds to reduce shear stress and support cell aggregate formation.
• BioBLU 5p bioreactors are equipped with the Eppendorf-exclusive packed-bed impeller, pre-loaded with Fibra-Cel Disks.
Non-invasive sensor technology reduces the contamination risk
• Temperature: The temperature sensor is inserted into the thermowell (RTD port) allowing non-invasive temperature measurement.
• Dissolved oxygen (DO): The gas-permeable membrane of the DO sensor port prevents direct contact of the sensor with the culture medium.
• pH: BioBLU c Single-Use Bioreactors are equipped with non-invasive optical pH sensing technology from PreSens®. Eppendorf provides optical pH modules for the use of optical pH measurement on bioprocess control systems that are not equipped with this technology. Some BioBLU c Single-Use Bioreactor size variants are available optionally without optical pH sensor technology. Both variants can be equipped with standard pH sensors as well.
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Applications
- Cultivation of mammalian and insect suspension cells.
- Cultivation of adherent cells as cell aggregates, on microcarriers, or on Fibra-Cel Disks.
- For the cultivation of microbial cells we offer the BioBLU f Single-Use Bioreactor product line.
- BioBLU Single-Use Bioreactors for cell culture applications are typically used for example in research and process development in the fields of cell and gene therapies, monoclonal antibodies and other biologics, vaccines, and modern food.
Features
- Single-use, stirred-tank, rigid-walled bioreactors available in sizes ranging from 100 mL to 40 L working volume
- All BioBLU c bioreactor sizes available with pitched-blade impellers for cell culture applications
- BioBLU 0.3sc bioreactors are designed for stem cell applications in research and development
- BioBLU 5p bioreactors with Eppendorf-exclusive packed-bed impeller, pre-loaded with Fibra-Cel® Disks
- Unique non-invasive pH and DO sensor technology drastically reduces contamination risks (industry standard autoclavable pH sensors available for pH measurement)
- Sealed magnetic drive with fully enclosed bearings maintain vessel sterility
- Bioreactors are assembled with sparger, overlay, gas filters for inlet and exhaust as well as penetrations for pH, DO, temperature, liquid additions, sampling, and harvest
- For use with Eppendorf benchtop bioreactor systems
- Adaptor kits for legacy bioreactor controllers available
