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Cell Culture FAQ: How to handle various cell lines?
Lab Academy
- Cell Biology
- Cell Culture
- CO2 Incubators
- Cell Culture Consumables
- Essay
Cell culture work often implies the cultivation of various cell lines for different ongoing experiments. The simultaneous nourishment of different cell lines in a biosafety cabinet might save some time during a busy day in the laboratory but dramatically increases the risk of cross-contamination and can thus seriously impair the entire experiment. Therefore, some aspects should be considered when working with different cell lines:
- Work with only one cell line at a time in the biosafety cabinet.
- Use labeled aliquots of media and reagents for each individual cell line.
- Disinfect the interior of the biosafety cabinet after a work session with one cell line.
- Run the laminar flow for a few minutes between work sessions with different cell lines.
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