The limiting factor in low-volume real-time PCR (qPCR) is often the remaining fluorescence intensity. Eppendorf twin.tec real-time PCR plates are specially manufactured for qPCR, as they offer all the advantages of regular twin.tec plates, but with the added advantage of white wells. These reflect fluorescence much better than clear or frosted wells, so lower levels of fluorescence are still detectable with the same instrument – just by using the right consumables. The qPCR plates' white wells also significantly reduce interfering background fluorescence and lead to increased consistency between replicates, and giving highly reproducible results.
The wells are made from polypropylene – which guarantees fast heat transfer for fast assays – and the frame is made of rigid polycarbonate, to ensure easy and reliable handling – both in manual and automated assays.
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Eppendorf twin.tec® real-time PCR Plates - qPCR Plates
PCR plates dedicated to qPCR
Eppendorf twin.tec® real-time PCR plates (96 wells, stackable)
Underside of Eppendorf twin.tec® real-time PCR plate
Underside of a 96-well qPCR plate
Open Forensic DNA Grade qPCR plate
Avoid contamination during storage with individually wrapped forensic DNA grade qPCR plates
Forensic DNA Grade qPCR plate wrapped
Forensic DNA grade qPCR plates are individually wrapped to prevent contamination
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The white wells of Eppendorf’s twin.tec® real-time PCR plates increase the reflection of fluorescent light in real-time PCR (qPCR) and other fluorescence assays. The increased fluorescence from these specialized qPCR plates improves the sensitivity of your assays, while also improving reproducibility and consistency of your results.-
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High fluorescence intensity is instrumental in improving sensitivity in qPCR. This graph shows an up to 10-fold higher fluorescence intensity in twin.tec® real-time PCR plates compared to frosted wells.
Eppendorf twin.tec® real-time PCR plates reduce Ct values more effectively than other tested plates. On average, Ct values were 0.8 cycles lower (compared to clear wells), indicating better sensitivity.
White wells improve homogeneity of results over the block. In this graph you can see the reduced standard deviation over the block compared to other plates.
Downloads: Eppendorf twin.tec® real-time PCR Plates
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