Run two independent protocols simultaneously on one PCR thermal cycler with the Eppendorf Mastercycler® nexus X2. The 32-well block is well suited for small assays, whereas larger assays can be run on the 64-well block, which is available with a gradient function.
Like every member of the Mastercycler nexus family, the Mastercycler nexus X2 can be combined with all other family members in a network of up to three units. In combination with Eppendorf’s PCR tubes, PCR strips or divisible plates, the Mastercycler nexus X2 will give you consistent, publication-ready results – every day!
The flexlid® concept for effortless height adjustment
The flexlid concept enables the nexus X2 PCR thermal cycler to deal with all types of consumables, from low volume all the way up to 0.5 mL PCR tubes. Of course, all common PCR plates fit – and if you ever need higher throughput, you can simply connect one or two Mastercycler nexus X2 eco units to your Mastercycler nexus.
Gradient with SteadySlope® technology
The Mastercycler pro’s gradient option features SteadySlope technology to ensure that heating and cooling ramp rates are identical in both gradient and normal operation. This gives you identical temperature control characteristics in both optimization and routine experiments. Programming the gradient with the Control Panel and the CycleManager pro is easy and intuitive, so that new users can get started quickly and safely.
Certified quality
All Eppendorf cyclers follow these quality guidelines:
• Individual quality control certificates
• Calibration according to national and international standards, e.g. NIST (USA), DKD/PTB (Germany), UKAS/NPL (UK)
• UL/cUL listed
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Mastercycler® nexus X2 - PCR Thermal Cycler
Eppendorf Mastercycler nexus X2 enables two separte PCRs in one device
Mastercycler® nexus X2 is well suited for busy PCR labs
The Eppendorf Mastercycler nexus X2 in a lab
Mastercycler® nexus X2: The double-block PCR cycler from Eppendorf
A Little Helper Eppendorf Mastercycler GX2e
The Mastercycler GX2e can be attached to a Mastercycler® nexus for higher PCR throughput
Perfect Asymmetry of the Mastercycler nexus X2 angled view
Benefit from a 1–12 °C gradient with the Mastercycler® nexus GX2
If you need that extra throughput
Increase productivity even further by connecting up to three Mastercycler® nexus X2 units
Perfect Asymmetry of Eppendorf Mastercycler® nexus
Large block for large assays, small block for small assays. The choice is yours.
Product Information
The Mastercycler® nexus X2 is Eppendorf's solution for busy PCR labs. Two totally independent protocols can be run on the two blocks, so two PCR users can independently start and end their PCR reactions whenever they want. One of the assays can even have up to 64 samples. With less than 40 dB[a] noise emission, the nexus PCR cycler is whisper quiet and also very energy efficient.Mastercycler-Nexus-GX2
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Learn more about sustainability at Eppendorf - incl. how to recognize a sustainable PCR thermocycler
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Find your cycler's best consumable match
You will find additional download material at the bottom of this page
Products (4)
Services (8)
Accessories (4)
VisioNize (2)
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Product Information
Technical Data
Applications
Features
Product Information
Improving ergonomics across the lab
Ergonomics goes far beyond just the ergonomically designed chair. At Eppendorf, we began optimizing our laboratory devices for ergonomics in the early 1970s.
In 2003, we launched the PhysioCare Concept®, focusing on ergonomic liquid handling devices, like our pipettes.
Since then we’ve broadened the Eppendorf PhysioCare Concept to include many laboratory products, including PCR cyclers. Our aim is to offer a holistic solution for harmonizing the workflow in your laboratory with your health and wellbeing.
For the Eppendorf Mastercyler range, this means:
• Ergonomically designed lid closure
• Low noise level for convenient working conditions at the bench
• Small footprint to enable position in direct arm reach at your bench
• Standardized user-interface concept for intuitive handling of devices
• Guided software for easy handling
Further information about the Eppendorf PhysioCare Concept:
www.eppendorf.com/physiocare/
Simple, intuitive documentation and tracking
The eLABJournal® Electronic Lab Notebook offers an intuitive and flexible solution to document research and track research data, including optimal PCR cycler temperatures and results. Improve efficiency when documenting, organizing, searching, and archiving collected data. With the free eLABJournal add-ons, you can extend the functionality of eLABJournal to a fully integrated Laboratory Information Management System.
Start a free 30-day trial!
Visit www.eLABJournal.com
Efficient lab and device management with VisioNize® Lab Suite
Are you interested in monitoring your lab devices and receiving notifications e.g., for device alarms? A digital, connected lab offers even more.
Gain access to a modular range of digital services and choose what works for your lab. VisioNize Lab Suite offers everything you need as a Lab Manager or Scientist to get started to digitally manage your lab for increased sample security, compliance needs and maintenance management.
Chart your own course to digital lab excellence! Find out more
Disposal of packaging
The packaging of the PCR cycler contains different materials, this includes cardboard, a plastic dust cover, and some foam parts. Recycling of materials becomes more and more important every day: Are you aware that nearly all cardboard material is recycled in Europe? Our cycler packaging cardboard material consists of approx. 60 % recycling material. Please support the global sustainability initiative of recycling valuable raw material by also collecting the cycler cardboard packaging material and disposing of it in the appropriate collection container at your organization. In respect to the plastic dust cover made of LD-PE and the foam parts, we recommend to select a dedicated recycling partner where PE material can be recycled. We suggest you contact your local waste hauler or facility management team to understand the available recycling options for your organization.
Disposal of instrument
Our cyclers last for many years, but if they need to be replaced, we kindly ask you to fulfill local requirements for disposal of these instruments. We strongly recommend a certified local recycling partner with experience in instruments with electronics. Keeping it "local" reduces the impact of transportation, and the "certified" aspect is recommended due to the safe and sustainable removal of specific parts.
Decontamination
This piece of equipment was used in a laboratory and/or was used to handle biological samples. Please keep in mind to adequately decontaminate the equipment which needs to be disposed. Check local requirements. For more information, get in contact with your local biosafety officer and/or waste officer. Check if your local recycling partner has special instructions and/or documentation requirements. You may also use the Eppendorf decontamination form sheet as guidance.
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Technical Data
| Specifications | Mastercycler® nexus GX2 | Mastercycler® nexus X2 | Mastercycler® nexus GX2e |
|---|
| Unit can only be controlled1) | – | – | ■ |
| Thermoblock | Aluminum | Aluminum | Aluminum |
| Sample capacity | 64/32 × 0.2 mL PCR tubes or up to 24/10 × 0.5 mL PCR tubes | 64/32 × 0.2 mL PCR tubes or up to 24/10 × 0.5 mL PCR tubes | 64/32 × 0.2 mL PCR tubes or up to 24/10 × 0.5 mL PCR tubes |
| Heating technology | Peltier elements, Triple Circuit Technology | Peltier elements, Triple Circuit Technology | Peltier elements, Triple Circuit Technology |
| Temperature control mode | Fast, Standard, Safe | Fast, Standard, Safe | Fast, Standard, Safe |
| Temperature control range of the block | 4 – 99 °C | 4 – 99 °C | 4 – 99 °C |
| Intuitive graphic programming | ■ | ■ | – |
| Gradient range | 1 – 12 °C | − | 1 – 12 °C |
| Gradient temperature control range | 30 – 99 °C | − | 30 – 99 °C |
| Block homogeneity 20 – 72 °C | ≤ ±0.3 °C | ≤ ±0.3 °C | ≤ ±0.3 °C |
| Control accuracy | ±0.2 °C | ±0.2 °C | ±0.2 °C |
| E-mail Notification | − | ■ | ■ |
| Product type | PCR | PCR | PCR |
| flexlid® concept | ■ | ■ | ■ |
| 2 years warranty | ■ | ■ | ■ |
| Universal Block for 0.2 mL and 0.5 mL vessels | ■ | ■ | ■ |
| Gradient block | ■ | − | ■ |
| Unit can control 2 other units in network | ■ | ■ | – |
| USB port | ■ | ■ | – |
| Block homogeneity 90 °C | ≤ ±0.4 °C | ≤ ±0.4 °C | ≤ ±0.4 °C |
| Heating rate | max. 3 °C/s2) | max. 3 °C/s2) | max. 3 °C/s2) |
| Cooling rate | max. 2 °C/s2) | max. 2 °C/s2) | max. 2 °C/s2) |
| Gradient block | over 8 rows (0.2 mL) | − | Over 8 rows |
| Lid descent | flexlid® technology, with thermal sample protection | flexlid® technology, with thermal sample protection | flexlid® technology, with thermal sample protection |
| Lid temperature range | 37 – 110 °C | 37 – 110 °C | 37 – 110 °C |
| Interfaces | CAN_in/CAN_out, Ethernet, USB | CAN_in/CAN_out, Ethernet, USB | CAN_in/CAN_out, Ethernet |
| Power supply | 100 – 110 V, 50 – 60 Hz | 100 – 110 V, 50 – 60 Hz | 100 – 110 V, 50 – 60 Hz |
| Max. power consumption | 700 W | 700 W | 700 W |
| Total weight | 10.5 kg | 11 kg | 10.5 kg |
| Dimensions (W × D × H) | 25 × 41.2 × 33 cm / 9.8 × 16.2 × 13 in | 25 × 41.2 × 33 cm / 9.8 × 16.2 × 13 in | 25 × 41.2 × 33 cm / 9.8 × 16.2 × 13 in |
| Number of programs on device | > 700 | > 700 | > 700 |
| Remote Device Monitoring and Notifications (VisioNize®) | VisioNize® box enabled | VisioNize® box enabled | VisioNize® box enabled |
1)Control through a Mastercycler® nexus, Mastercycler® nexus gradient, Mastercycler® nexus GSX1, Mastercycler® nexus SX1, Mastercycler® nexus GX2, Mastercycler® nexus X2 or Mastercycler® nexus flat
2)measured at the block
Applications
- Standard PCR
- PCR optimization
Features
- Asymmetrically divided block for running different assays in parallel, one 32-well and one 64-well block
- Intuitive graphic programming
- Universal block for strips, 0.1 mL, 0.2 mL and 0.5 mL PCR tubes and fits for divisible PCR plates
- Small footprint
- Optional gradient for PCR optimization
- E-mail notification
- flexlid® concept allows use of all types of consumables with automatic height adjustment of the lid
