Are Eppendorf products free of RNA?

All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.

What is the meaning of the PCR Clean seal?

For molecular-biological requirements Eppendorf offers a new standard for uniform purity specifications. All consumables marked with the "Certified PCR Clean” label are guaranteed free of human DNA, DNase, RNase and PCR inhibitors.

Why are there double labels on the folding boxes, as "PCR clean" + "Sterile" or "PCR clean" + "Protein-free"?

Due to the modularization of purity grades, some products are labeled with more than one purity grade symbol. For example, the epDualfilter T.I.P.S. has the double seal "PCR clean "+ "Sterile" and the UVette the double seal "PCR clean" + "Protein-free" on the packing. The certification remains unaffected by the modularization.

Are lot specific certificates for the purity grades available in the internet?

Yes, the lot specific purity certificates, so-called Test report, can be downloaded at the certificates homepage .

Can products of the previous and new modular purity grades (with new seals) be used simultaneously in one application?

Yes, if no batch attachment is required for the application, products of the old and new modular purity grades can be used, as their specifications are the same.

Can the lid of the Standard Micro Test Tube 3810X be pierced as easily as that of the Safe-Lock micro test tube?

No. It is more easy to pierce a Safe-Lock Tube because of the thin membrane in the middle of the lid.

What is the life expectancy of Eppendorf Tubes?

At least 5 years at a storage temperature of +5 to +35 °C. If unopened and properly stored Eppendorf Tubes have shelf-life of 8 Years (standard Eppendorf Quality) or 5 Years on a purity grade (Biopur, PCRclean, Sterile, Forensic DNA Grade, ). Please observe " Instruction for Use" for storage condition recommendations: - Instruction for Use (IFU) of Tubes - Conical tubes, SafeCode tubes and SnapTec tubes - Instruction for Use of 5 mL Tubes - Instruction für Use of LoBind Tubes

From what material are Eppendorf Tubes manufactured?

All Eppendorf Tubes are manufactured from virgin polypropylene. The used polypropylene (PP) fulfils the specifications of the FDA for the use of plastics for foodstuffs. During production, no slip agents, biocides, or plasticizers are used. Colorants are free of biological material, free of heavy metals, and free of melamine. No metal-based dyes are used. Please see General Quality Certificate for epT.I.P.S, Combitips ® advanced, ViscoTip, Tubes, Plates, UVette.

Do you offer siliconized micro test tubes?

Our tube assortment does not include coated or siliconized tubes. In order to keep sample loss caused by the adsorption of sample material on the tube wall to a minimum, we recommend using the Eppendorf Protein LoBind Tubes or Eppendorf DNA LoBind Tubes. These are made of a special type of polypropylene and are manufactured under optimized process conditions.

Can Eppendorf micro test tubes be sterilized with ethylene oxide?

Yes, this is possible. Please note that the micro test tubes have to be taken out of the packaging for sterilization. In addition, please note that some ethylene oxide will remain in the material after sterilization.

Can your micro test tubes be frozen at -80 °C?

Yes. Please refer to the operating manual.

Can your micro test tubes be coated with acrylic resin within the framework of a UV polymerization?

Yes, the tubes can withstand UV polymerization. However, we cannot give you any instructions for the procedure.

Can autoclaving inactivate bacterial endotoxin contamination in Eppendorf Tubes?

No. Bacterial endotoxins (lipopolysaccharide, LPS) are very stable and withstand normal autoclaving at 121 °C. To inactivate LPS, the objects have to be baked at 250 °C, which, of course, is not possible with plastics.

Are the Eppendorf Tubes anti-static?

Eppendorf tubes, including SafeCode tubes and all biobased tube variants, are not produced from antistatic material, i.e. they are basically not antistatic. But it should be noted that the use of antistatic agents during production will destroy or reduce the wettability of the tubes.

Which Eppendorf micro test tubes are free of DNA?

All micro test tubes designated with "PCR clean" are free of human DNA, DNase, RNase and PCR inhibitors. Our Safe-Lock micro test tubes are also available in the "Eppendorf Biopur" purity standard. Due to the special manufacturing processes and the individual blister-packaging, Biopur products are certified "DNA free".

How do you define the temperature entry "RT", for example, as a storage condition for your micro test tubes?

Definition of RT (room temperature): +15 to +25 °C.

What is the best way to label Eppendorf Tubes?

Tubes should be clean, free from grease and only labeled on the space provided. Water-resistant felt pens from Steadtler S and F, in black, are the most suitable for this. The writing should be left to dry for at least 2 min.

Do Eppendorf Tubes contain heavy metals?

Heavy metal values are available in the general "Certificate of Quality - Tubes - Trace metal". All the values are below the prescribed limits of the listed DIN norm.

Overview regarding the centrifugation resistance of Eppendorf Tubes

Please consult the "Instruction for Use" of the respective Tube.

Are the Eppendorf tubes autoclavable?

Yes, all Eppendorf tubes are autoclavable when open (20 min, 121 °C, 1 bar).

Are Eppendorf tubes and plates (also LoBind variants) resistant to 100% acetonitrile?

Eppendorf tubes and plates including LoBind variants are resistant against 100% acetonitrile only in room temperature conditions. Above 40°C and prolonged exposure (days) may lead to partial damage and is not recommended. For lower acetonitrile concentrations the resistance parameters will be better so prolonged exposure and higher temperatures may be possible. This must be however tested by the customer.

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Flex-Tube^® - Microtube

Q: Is there a chemical resistance overview for the individual Tube types?

Yes. The Eppendorf Application Note 056 "The Best Material for Original Eppendorf Tubes ® and Plates: Properties and Chemical Resistance of Polypropylene" includes among other things a detailed material stability overview.

Colorless Flex-Tube® microtube with easy-open lid

Flex-Tube® microtube in blue with easy-open lid

Flex-Tube® microtube in green with easy-open lid

Product Information

The easy-open, easy-close design of the Flex-Tube^® provides convenient, ergonomic, and reliable one-handed operation. Whether sample preparation, centrifugation or storage – the Flex-Tube^® fit perfectly into your workflows!

Show more Product Information

You will find additional download material at the bottom of this page

Product Information

Features

The easy-to-open, securely closing, Flex-Tube^® offers comfort and dependability for sample preparation, centrifugation, and storage. One vessel for all applications in molecular biology, chemistry, and biochemistry.
Safer than ever: Flex-Tube^® are designed for centrifugation up to 30.000 x g!

Best material!

Eppendorf consumables incl. the Flex-Tube^® are made exclusively from high quality, ultra pure polypropylene, which complies with FDA guidelines 21 CFR § 177.1520 »Olefin Polymers«, and 21 CFR § 178.2010 »Antioxidants and Stabilizers for Polymers«. Extensive quality checks are in place prior to and during production. Furthermore, the final product is examined for functionality. The functionality tests for tubes include lid closure security in a boiling water bath, centrifugation stability and vapor tightness. These quality controls and tests ensure the sustained high product quality across the entire production phase – lot by lot. Please see APPLICATION NOTE No. 056 for details: The best material for original Eppendorf Tubes^® and Plates: Properties and chemical resistance of polypropylene

Highest sample integrity!

Manufactured without the use of slip agents, plasticizers, and biocides – substances that have been shown to leach from plastic consumables into the sample and negatively affect bio–assay results or measurements, e.g. nucleic acid quantification by photometric measurements at 260 nm.

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Downloads: Flex-Tube^®

Brochure

Absolute Tubes

Your best choice to care for your sample: Eppendorf Tubes

PDF 2.6MB

English

Flyer

Eppendorf Purity Grades Selection Guide

Eppendorf Forensic DNA Grade according to ISO 18385

PDF 1.3MB

English

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Flex-Tube® - Microtube

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Colorless Flex-Tube®microtube with easy-open lid