Are Eppendorf products free of RNA?

All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.

What is the meaning of the PCR Clean seal?

For molecular-biological requirements Eppendorf offers a new standard for uniform purity specifications. All consumables marked with the "Certified PCR Clean” label are guaranteed free of human DNA, DNase, RNase and PCR inhibitors.

Why are there double labels on the folding boxes, as "PCR clean" + "Sterile" or "PCR clean" + "Protein-free"?

Due to the modularization of purity grades, some products are labeled with more than one purity grade symbol. For example, the epDualfilter T.I.P.S. has the double seal "PCR clean "+ "Sterile" and the UVette the double seal "PCR clean" + "Protein-free" on the packing. The certification remains unaffected by the modularization.

Are batch-specific certificates for the new purity grades available in the internet?

Yes, the batch-specific purity grades can be downloaded at our certificates homepage .

Can products of the previous and new modular purity grades (with new seals) be used simultaneously in one application?

Yes, if no batch attachment is required for the application, products of the old and new modular purity grades can be used, as their specifications are the same.

Do the Eppendorf LoBind Tubes have the same material durability as all other micro test tubes from Eppendorf?

The Eppendorf LoBind Tubes completely satisfy the specifications of the Eppendorf Standard Safe Lock tubes with reference to centrifugation stability (exception: Protein LoBind Tube), lid tightness, pop up safety and chemical resistance. (See Application No. 56: The best material for original Eppis.)

Are the Eppendorf LoBind Tubes coated?

Not the Eppendorf LoBind Tubes are neither siliconized nor coated. The low binding affinity results from of a special type of polypropylene and the optimized manucturing process.

Can I use the Protein LoBind Tubes regardless of whether I am working with basic or acidic proteins?

The Protein LoBind tubes show protein low binding properties for both low and high pH values.

What distinguishes the Protein LoBind Tube from a "Standard" Safe-Lock Eppendorf tube?

The Protein LoBind Tube shows, in comparison to a Safe-Lock Tube, significantly less protein binding affinity. The Protein LoBind Tube is therefore especially suitable for applications with the lowest quantities of protein.

Are the Eppendorf LoBind Tubes autoclavable?

Please note that only the LoBind Tubes with snap can are autoclaveable when opened: 121°C, 20 min. LoBind Tubes with screw cap are not autoclaveable.

Are Eppendorf tubes and plates (also LoBind variants) resistant to 100% acetonitrile?

Eppendorf tubes and plates including LoBind variants are resistant against 100% acetonitrile only in room temperature conditions. Above 40°C and prolonged exposure (days) may lead to partial damage and is not recommended. For lower acetonitrile concentrations the resistance parameters will be better so prolonged exposure and higher temperatures may be possible. This must be however tested by the customer.

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Protein LoBind^® Tubes

DNA LoBind® tubes - 0.5 mL, 1.5 mL, 2 mL and 5 mL with closed lid

Eppendorf LoBind® tube with open lid and volume marker

Eppendorf LoBind® tubes - 0.5 mL, 1.5 mL, 2 mL and 5 mL

Eppendorf LoBind® conical tubes - 15 mL and 50 mL, closed lid

Scientist pipetting into Eppendorf 5 mL microtubes® stored in rack

Conical Tubes 15 mL, Protein LoBind in bag

제품 정보

Eppendorf Protein LoBind Tubes는 단백질체학 실험이나 기타 단백질 연구 분야에서 단백질 회수율을 극대화합니다. 단백질 시료 회수율을 높여 정확하게 다운스트림 분석을 수행하고 효소 열화를 방지하십시오.

제품 정보 자세히 표시

이 페이지 하단에서 추가 다운로드 자료를찾을 수 있습니다.

제품 정보

애플리케이션

특징

Eppendorf Protein LoBind Tubes를 사용해 표면에 결합되어 손실되는 시료를 최소화할 수 있습니다. 혁신적인 2성분 고분자 mix가 친수성 표면을 생성해 단백질 회수율과 다운스트림 분석이 크게 개선됩니다.
표준 vessel을 사용할 경우 시료가 플라스틱 표면에 결합되어 24 시간 안에 시료의 90 % 이상이 손실될 수 있습니다. Eppendorf LoBind Tubes를 사용하면 더 이상 시료 손실을 걱정할 필요가 없습니다!

Eppendorf Protein LoBind^® 제품

Protein LoBind^® 제품은 tube, microplate, deepwell plate 등 다양한 타입으로 제공되어 사용자의 요구사항을 완벽히 충족합니다. 시료량을 줄이든지 아니면 처리량을 늘리든지 상관없이 LoBind가 도움이 됩니다.

탁월한 단백질 회수율

표준 vessel에 비해 90 % 이상 단백질을 회수합니다! Eppendorf Protein LoBind^® Tubes는 96 시간 동안 배양한 후 경쟁업체의 low binding tube보다 훨씬 더 높은 회수율을 제공합니다. 자세한 정보는 Application Note 180을 참조하십시오.

MALDI-TOF 중 신호 강도를 높이는 Protein LoBind^® Tubes

Protein LoBind^® Tubes는 MALDI-TOF 분석 중에 신호 강도를 높여 일반적으로 검출 불가능한 것으로 간주되는 농도(1 pmol)에서 검출과 분석이 가능합니다. 이 그래프에서 화살표는 각 실험의 신호를 나타냅니다. (출처: Dr. S. Seeber and Dr. Humeny, Institute of Biochemistry, University of Erlangen-Nürnberg, Germany, 자세한 정보는 Application Note 180 참조).