Are Eppendorf products free of RNA?

All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.

What is the meaning of the PCR Clean seal?

For molecular-biological requirements Eppendorf offers a new standard for uniform purity specifications. All consumables marked with the "Certified PCR Clean” label are guaranteed free of human DNA, DNase, RNase and PCR inhibitors.

Why are there double labels on the folding boxes, as "PCR clean" + "Sterile" or "PCR clean" + "Protein-free"?

Due to the modularization of purity grades, some products are labeled with more than one purity grade symbol. For example, the epDualfilter T.I.P.S. has the double seal "PCR clean "+ "Sterile" and the UVette the double seal "PCR clean" + "Protein-free" on the packing. The certification remains unaffected by the modularization.

Are lot specific certificates for the purity grades available in the internet?

Yes, the lot specific purity certificates, so-called Test report, can be downloaded at the certificates homepage .

Can products of the previous and new modular purity grades (with new seals) be used simultaneously in one application?

Yes, if no batch attachment is required for the application, products of the old and new modular purity grades can be used, as their specifications are the same.

What distinguishes the DNA LoBind Tube from a "Standard" Safe-Lock Tube?

In comparison to a Safe-Lock Tube, the DNA LoBind Tube shows significantly less DNA bonding affinity. This effect is most apparent with high salt concentrations and when working with short DNA fragments. The DNA LoBind Tube is therefore especially suitable for applications that should be carried out with the minimum loss of nucleic acid material.

Do the Eppendorf LoBind Tubes have the same material durability as all other micro test tubes from Eppendorf?

The Eppendorf LoBind Tubes completely satisfy the specifications of the Eppendorf Standard Safe Lock tubes with reference to centrifugation stability (exception: Protein LoBind Tube), lid tightness, pop up safety and chemical resistance. (See Application No. 56: The best material for original Eppis.)

Are the Eppendorf LoBind Tubes coated?

Not the Eppendorf LoBind Tubes are neither siliconized nor coated. The low binding affinity results from of a special type of polypropylene and the optimized manucturing process.

What distinguishes the Protein LoBind Tube from a "Standard" Safe-Lock Eppendorf tube?

The Protein LoBind Tube shows, in comparison to a Safe-Lock Tube, significantly less protein binding affinity. The Protein LoBind Tube is therefore especially suitable for applications with the lowest quantities of protein.

Are the Eppendorf LoBind Tubes autoclavable?

Please note that only the LoBind Tubes with snap can are autoclaveable when opened: 121°C, 20 min. LoBind Tubes with screw cap are not autoclaveable.

Are Eppendorf tubes and plates (also LoBind variants) resistant to 100% acetonitrile?

Eppendorf tubes and plates including LoBind variants are resistant against 100% acetonitrile only in room temperature conditions. Above 40°C and prolonged exposure (days) may lead to partial damage and is not recommended. For lower acetonitrile concentrations the resistance parameters will be better so prolonged exposure and higher temperatures may be possible. This must be however tested by the customer.

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DNA LoBind^® Tubes

Sample being pipetted from elongated ep T.I.P.S.® to Eppendorf LoBind® deepwell plate

Scientist pipetting into Eppendorf 5 mL microtubes® stored in rack

DNA LoBind® tubes - 0.5 mL, 1.5 mL, 2 mL and 5 mL with closed lid

Eppendorf LoBind® tube with open lid and volume marker

Eppendorf LoBind® tubes - 0.5 mL, 1.5 mL, 2 mL and 5 mL

Small section of Eppendorf DNA LoBind® microplate

제품 정보

핵산 회수율을 극대화하세요! Eppendorf LoBind^® Tubes는 표면에 결합되는 시료를 크게 감소시켜 DNA 및 RNA 분자의 회수율을 극대화합니다. 따라서 시료 전처리와 핵산의 장기 보관에 이상적인 솔루션입니다!

제품 정보 자세히 표시

이 페이지 하단에서 추가 다운로드 자료를찾을 수 있습니다.

제품 정보

애플리케이션

특징

Eppendorf LoBind^® Tubes를 사용해 시료 손실을 최소화하고 핵산 회수율을 극대화하십시오. 첨단 제조 기술을 이용해 고품질 폴리프로필렌으로 제조하는 LoBind^® tube는 표면 코팅 없이 DNA 및 RNA 분자를 거의 100 % 회수합니다.
최고 품질의 Eppendorf LoBind^® Tubes는 법의학 분석, microarray, 차세대 염기서열 분석(NGS) 등 모든 실험에 적합한 솔루션입니다. LoBind^® tube는 독립 실험실에서 엄격한 배치 테스트와 인증 과정을 거치기 때문에 'free from DNA, DNase, RNase 및 PCR inhibitor'가 보장됩니다.

모든 문제를 해결하는 Eppendorf LoBind^® 솔루션!

Eppendorf DNA LoBind^®는 표준 tube에서 microplate와 deep well plate에 이르는 다양한 타입으로 제공되어 시료량과 처리량에 상관없이 사용자의 요구사항을 충족합니다.

탁월한 DNA 회수율

Eppendorf LoBind^® Tubes는 배양 온도와 시간에 상관없이 DNA의 최대 99 %를 회수합니다. 그래프는 다양한 조건으로 배양한 후 Eppendorf LoBind^® Tubes와 경쟁업체 tube의 DNA 회수율(2.5 M NaCl/TE buffer에서 0.2 ng/µL DNA fragment (130 bp, 32P labeled))을 보여줍니다.

Eppendorf LoBind^® Tubes는 경쟁업체의 low bind tube보다 시료 회수율이 우수합니다. real-time PCR absolute quantification을 위한 표준물질의 준비 및 보관에서 Eppendorf LoBind^® Tube의 일관성을 평가한 결과, LoBind^® Tubes에 흡수되는 DNA의 양이 적어 assay된 시료에서 표준 곡선과 DNA quantification의 품질이 개선되었습니다.

이미지: DNA 표준물질 준비: 게놈 DNA 스톡 용액을 경쟁업체 low bind tube나 Eppendorf DNA LoBind^® Tubes에서 10배 희석했습니다. real-time PCR로 분석하기 전에 –80 °C에서 24시간 동안 각 희석액의 aliquot을 22 µL씩 보관했습니다. Eppendorf LoBind^® Tubes에 보관된 시료의 PCR efficiency가 경쟁업체 제품보다 우수했습니다. 자세한 정보는 Application Note 226을 참조하십시오.