How does the shaker agitation rate and orbit affect culture growth?

Generally, agitation speed and orbit affect both the aeration and the mixing of the culture. Greater aeration increases oxygen transfer rate (OTR), boosting cell growth. The size of the orbit affects OTR linearly and the speed has a square-law relationship. Therefore, if the size of the orbit doubles, say from 1/2 to 1," the amount of OTR should double〖RPM〗_1/〖RPM〗_2 = (r_2^2)/(r_1^2 ), then 〖RPM〗_2 = √(〖RPM〗_1^2 (r_1/r_2 ) ). If the speed doubles, the OTR should quadruple. Of course, these are general rules and caution should be taken with shear sensitive cells. However, this information can be used to estimate the conditions needed to obtain the same results on different model shakers. For example, to get the same oxygen transfer in a shaker with a 1 in orbit running at 300 rpm, a shaker with 3/4 in orbit must be run at 350 rpm.

Do Eppendorf shakers come with platforms?

Each Eppendorf shaker is available with multiple platform options, which are ordered separately to give you the flexibility to choose the one that best suits your needs. Dedicated platforms include installed clamps of a single size (ie. only 250 mL clamps) and provide in most cases the best capacity but does not allow for the use of different size clamps or holders. Universal Platforms are designed for use with a combination of various-sized clamps and test tube racks providing maximum flexibility. When ordering a Universal Platform, clamps and test tube racks must also be ordered separately.

What is the temperature offset function?

The temperature offset function on many of our shakers allow you to match a reference point" temperature inside the chamber (culture medium) with the "Actual Value" displayed. The temperature offset function on Innova Incubated shakers allows you to monitor the temperature of the media providing you a clearer and more accurate picture. Please be aware that matching reference point temperatures to the Actual means you have to deal with many variables such as: greater evaporation; heat gains generated by cultures growing - (slowly at first and much more during their growth phase) and heat generated by the friction of media with the glass at high speeds or use of baffled flasks. Over time, the reference point temperature offset may need to change due to these varying conditions.

I want to grow my cultures at 27°C, do I need an incubated shaker with refrigeration?

The Eppendorf Incubated, non-refrigerated Shakers Innova and I-Series are designed to maintain temperatures of ambient plus 5 °C to its maximum operating temperature. The Eppendorf Incubated Excella shakers will operate from ambient plus 7 °C to maximum temperature. It is critical to understand that "ambient temperature is not the temperature on the wall thermometer (room temperature), but the temperature measured one meter from the front of the machine.

Why are some flask clamps supplied with an extra spring set?

This "extra spring set" (we call it a girdle) is included with clamps for 2 L, 2.8 L, 4 L and 6 L flasks. It is intended to be used with the already installed spring set. This girdle helps prevent large flasks from spinning inside the clamp and reduces the risk of flasks becoming dislodged or scored by the steel clamp. One girdle is already in place on the clamp, the other is packed separately. To install this extra “girdle”, follow the instructions in the manual supplied with the shaker.

How is temperature accuracy defined by Eppendorf?

Accuracy is defined as the degree of closeness between the displayed value and the actual value as determined by a traceable device. All Eppendorf Incubated Innova and I-Series shakers have a temperature Accuracy of ±0.1 °C from 30 – 40 °C, ±0.5 °C over the remaining range. New Brunswick Incubated Excella Shakers are accurate within 0.1°C at 37°C.

How is Uniformity defined by Eppendorf?

Uniformity is the ability to maintain uniform temepratures throughout the platform. New Brunswick Incubated Innova Shaker temperatures will not deviate by more than +/- 0.25 °C at 37 °C.

The orbit of a shaker is the distance traveled around the central drive. This can be descibed in inches, centimeters, or milimeters. If our shakers had a one inch (2.54 cm) orbit. That means that that the lateral movement of the platform would equal one inch from one point to another that is 180° opposite that position. All of the New Brunswick. shakers have a circular orbit

What wavelengths are used in your growth lamps in a photosynthetic light bank?

Each of the bulbs that is fit into our photobanks is a wide spectrum bulb that provides the red and blue wavelength of energy needed for photosynthesis. Innova 42R has six bulbs as the Innova 43R and 44R have nine bulbs. To have this option installed, the shaker needs to have the refrigeration option installed as well.

Which screws will fit to which platform ?

All of Eppendorf Inc. clamps and accessories are packaged with multiple screws to fix them to our universal platforms, included in M1013-0261 clamp screw kit (Stainless steel platform screw #S2117-3050 (10-32 x 5/16 in (7.9mm)), Stainless Steel, Aluminum, and Aluminum and Phenolic (Plastic) #S2116-3051 (10-24 x 5/16 in (7.9 mm)), and Wood #S2116-3101 (10-24 x 5/8 in (15.87mm)).

How can I disinfect, clean, maintain my shaker?

We recommend cleaning the exterior of our units with a damp cloth or any standard household or laboratory cleaner to wipe the surfaces clean. Do not use abrasive or corrosive compounds to clean the instruments as they may damage the unit and void the warranty. It is the responsibility of theuser to carry out the proper decontamination procedures if hazardous materials are spilled on or inside the equipment. Before using any cleaning or decontamination method other than those suggested by the manufacturer, users should contact Eppendorf Inc. to ensure that the proposed method will not damage the equipment. Commercially available household bleach solutions, when diluted at a 1:10 ratio, are effective in routine decontamination of the instrument. The method for decontaminating a spill depends on the nature of the spill. Spills involving fresh cultures or samples of known concentrations of biomass should be flooded with decontamination solution and soaked for at least 5 minutes before cleanup. Spills involving samples with high concentrations of biomass, or involving organic matter, or occuring in areeas warmer than ambient/room temperature, should be exposed to decontamination solution for at least one hour before being cleaned up. Personnel involved in the cleanup of any spill should wear gloves, safety glasses, and a laboratory coat or gown during the cleanup process. Respiratory protection should be considered for spills where aerosolization is suspected.

Which flask type fits in which clamp ?

Please see the Accessories for New Brunswick™ Shakers: Dimensions and Capacities in the Userguides section of the shaker product page.

Which vessel fits in Eppendorf Test tube racks ?

Please see the Accessories for New Brunswick™ Shakers: Dimensions and Capacities in the Userguides section of the shaker product page.

Is it possible to monitor relative humidity in the Innova 42, 43, 44? ?

Innova 42, 43, 44 can be equipped with a relative humidity sensor optionally (Humidity monitor). This option allows monitoring the actual humidity level in the chamber and indicates the value on the screen. The option does not allow humidity control. The humidity sensor can be retrofitted by Eppendorf Technical Service. If humidity monitoring is installed, the maximum temperature is 60 °C. The maximum reachable relative humidity is around 40 – 45 % rH (at 37 °C) by filling the spill pan.

Why is the triple eccentric drive special ? What is the difference between Innova and Excella Shakers drive ?

The Innova and I-series Triple eccentric drive consists of three individual bearings and eccentric shafts mounted in a triangular fashion for precise uniform circular motion at every point on the platform. The heavy duty drive has a single shaft that utilizes springs to aid in balancing the load. This drive works great with small to medium loads at lower speeds.

How can I shake MTPs ? Which parameters ?

Eppendorf shakers offer a variety of Micro Titer Plate holders that attach to our univeral platforms. Our shakers offer a variety of orbits up to 5.1 cm (2 in) and speeds up to 500 rpm. If mixing is what is needed at higher speeds and smaller orbits you can find Eppendorf Mixers.

How do I use of sticky pads/sticky tape?

Eppendorf Sticky Pads are recommended for use on our sticky pad platform or our sticky pad adapter kit (M1250-9504). On the S44i universal platform sticky pad can be used directly. The adapter kit is easily fixed to our universal platforms. Any size flask can be used with the sticky pad with a 1 in (2.54 cm) orbit up to 250 rpm, any size flask can be used with the 2 in (5.1 cm) orbit up to 200 rpm. To clean the sticky pad, just wash with soap and water. this will revive" the stickiness, but the sticky pad must be completely dry before replacing on the platform.

What is the heavy load platform ?

The Heavy Duty Platform (M1282-9928) is designed for use in the Innova 44 only. This platform is 0.95 cm thick alluminum plate that is recommended for shaking full loads of flasks greater than 1 L at or above 20 % fill volumes or flasks with baffles.

What is the orbit of a shaker?

An orbital shaker moves the platform in a circular shaking motion. The term orbit describes the diameter of this circular movement. Typical orbit sizes are 1.9 cm, 2.5 and 5 cm.

What orbit shall I take?

An orbital shaker is used to grow microorganisms or cells growing in suspension, like bacteria, yeast, fungal, insect, algae or plant cells. Orbital shaking enables a homogeneous distribution and aeration of the cultures by increasing the oxygen transfer rate (OTR). There are different influencing parameters on shaking performance, e.g. the shaker orbit, the applied speed, the vessel shape, the liquid viscosity and the filling volume. The 2.5 mm orbit is the standard solution which works well for most applications. For some applications, e.g. large vessels > 2 Liter, especially large volume Fernbach flasks or very shear sensitive cells, a larger shaking diameter with the consequence of a reduced speed can be beneficial.

I use two shakers with different orbits. Can I transfer my experimental conditions to a shaker with a different orbit?

Yes, the rpm can be adapted with help of the following formula .

What platform types are offered with the shakers?

Universal platform and a variety of dedicated platforms are available. University platforms offer maximum flexibility having a hole-pattern that allows using virtually any combination of clamps and other accessories. Dedicated platforms come complete with pre-installed clamps of one size. The hole-pattern of the dedicated platform is optimized for the use of one specific size of clamps. It allows in most cased more capacity but can only be used with that size of clamp. Please note that the platform has to be ordered in addition to the shaker.

Why should I have my Orbital Shaker serviced at regular intervals?

As with all products with moving parts, wear and tear is inevitable and can affect how consistently the product operates over a long period of time. Eppendorf products are engineered to minimize wear and tear, but a periodic service allows early identification and tracking of excessive wear before product performance is outside of acceptable tolerances. Regular servicing may also be a regulatory requirement of our users.

How often does my orbital shaker need to be serviced?

Eppendorf recommends a service once every 12 months. However, the regulatory requirements of our users vary greatly and can often be more frequent.

How much are the different orbital shaker service programs?

The cost depends on the product variant and on the level of service that is required. Please contact your local Eppendorf Service centre for a personal quotation. www.eppendorf.com/contact

What is IQ or OQ and what do I need them for?

Installation and Operational Qualification; the Installation Qualification documents that the product has been delivered and installed in accordance with manufacturer recommendations and guidelines. The Operational Qualification checks the correct functioning of a device in accordance with manufacturer recommendations and guidelines (as such, it can only be provided by the equipment manufacturer). IQ/OQ is primarily required in organizations that implement quality management processes but is available to all users.

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New Brunswick Innova^® 43/43R - Console Incubator Shaker

Product Information

Are you looking for a large-capacity console incubator shaker for easy handling of large shake flasks? Have a look at the Innova 43 which offers peace of mind 24/7 with the famous heavy-duty Innova triple eccentric drive. It also saves up to $3800 / 3200€ over ten years as there is no internal HEPA-filter that requires yearly replacement for optimal performance.

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New Brunswick Innova^® 43

non-stackable, 230 V ± 10 %/50 Hz (CN/EU), orbit 5.1 cm (2 in)

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Catalog no. M1320-0012

New Brunswick Innova^® 43R

refrigerated, non-stackable, 230 V ± 10 %/50 Hz (CN/EU), orbit 5.1 cm (2 in)

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Catalog no. M1320-0016

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Product Information

Technical Data

Features

The Innova 43 and 43R (refrigerated) shakers are the perfect choice for handling large volumes of cultures with precision and ease. The top loading design allows for vertical placement of the flasks. A pedal operated lid and a window in the sidewall overlooking the samples are examples of why our customers continue to trust our designs. The cast iron triple-eccentric counterbalanced drive is specifically designed to support high-speed applications and heavy workloads – the design has stood the test of time for over 70 years and continues to enable scientists today. The simple user interface allows for easy programming of speed, temperature, time and the VisioNize box can be added to enhance monitoring and adds notifications functionality. Regular servicing can be carried out in-situ, allowing for minimized downtime.

Efficient lab and device management with VisioNize^® Lab Suite

Are you interested in monitoring your lab devices and receiving notifications e.g., for device alarms? A digital, connected lab offers even more.

Gain access to a modular range of digital services and choose what works for your lab. VisioNize Lab Suite offers everything you need as a Lab Manager or Scientist to get started to digitally manage your lab for increased sample security, compliance needs and maintenance management.

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Specifications	Innova^® 43, orbit diameter 5.1 cm (2 in)	Innova^® 43R, orbit diameter 5.1 cm (2 in)

External height (with lid/door open)	165.1 cm (65 in)	165.1 cm (65 in)
Timer	0.01 – 99.59 h, continuous	0.01 – 99.59 h, continuous
Power supply	230 V ± 10 %, 50 Hz	230 V ± 10 %, 50 Hz
Dimensions (W × D × H)	115.6 × 81.3 × 103.1 cm / 45.5 × 20 × 40.6 in	115.6 × 81.3 × 103.1 cm / 45.5 × 20 × 40.6 in
Product weight	202 kg / 445 lb	216 kg / 475 lb
Temperature range	Ambient +10 °C to 80 °C	20 °C below ambient to 80 °C
Audible and visual alarms	yes	yes
Temperature uniformity	±0.25 °C at 37 °C	±0.25 °C at 37 °C
Available program modes	• Constant speed and temperature • Programmable multi-steps • RS-232 communication port	• Constant speed and temperature • Programmable multi-steps • RS-232 communication port
Drive mechanism	triple-eccentric counterbalanced drive	triple-eccentric counterbalanced drive
Benchtop (B), Under Bench (U), Floor (F) or Stackable (S)	F	F
Gassing manifold	yes	yes
Water/Humidity monitoring	yes	yes
Internal convenience light	yes	yes
Memory	non volatile with automatic power failure restart	non volatile with automatic power failure restart
Motor type	Solid state, DC brushless motor	Solid state, DC brushless motor
Photosynthetic lighting	no	yes
Platform size (cm/in)	76 × 46 cm (30 × 18 in)	76 × 46 cm (30 × 18 in)
Refrigerated	−	yes
Slide-out platform	no	no
Door opening	console style	console style
Speed range	25 – 500 rpm with 2.5 cm orbit; 25 – 300 rpm with 5.1 cm orbit	25 – 500 rpm with 2.5 cm orbit; 25 – 300 rpm with 5.1 cm orbit
Multi-step programming	yes	yes
Steps per program	15	15
Stored programs	4	4
cETLus and CE marked	yes	yes
UV germicidal lamp	−	yes
Capacity	large	large
Programming steps / storable programs	15/4	15/4
Remote Device Monitoring and Notifications (VisioNize^®)	VisioNize^® box enabled	VisioNize^® box enabled
Temperature range	10 °C above ambient to 80 °C	20 °C below ambient to 80 °C

Agitation
Orbit	5.1 cm (2 in)	5.1 cm (2 in)

Downloads: New Brunswick Innova^® 43/43R

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Incubator Shaker Guide

Innova^® and New Brunswick Lab Shakers from Eppendorf

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Incubator Shaker Portfolio

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New Brunswick Innova® 43/43R - Console Incubator Shaker

Innova 43 3

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Often Imitated, Never Duplicated

Product Information

Products (2)

epServices (17)

Accessories (13)

Spare Parts (3)

VisioNize (2)

Product Information

Efficient lab and device management with VisioNize® Lab Suite

Technical Data

Features

Downloads: New Brunswick Innova® 43/43R

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