What is the UVette made of?

The UVette is made of a UV-transparent plastic.

Can the UVette be used in spectrophotometers of other manufacturers?

In general this is possible by using an adapter which we offer for light beam heights of 8.5, 10, 15 and 20 mm. Choose the adapter according to the height of the measuring beam in your spectrometer. Please contact therefore the manufacturer of your spectrometer directly or please ask your local Eppendorf office for assistance.

Can I reuse the UVette?

We recommend that the UVette be discarded following the sample measurement in order to guarantee contamination-free analysis. Cleaning processes and/or multiple use of an UVette may damage the quality of the optical surfaces, particularly in the sensitive UV-range. However, the UVette may be used up to five times for UV measurements if the Uvette material is not damaged during the cleaning procedures.

How to measure in the right way with the UVette?

As with quartz glass cuvettes, it is important to make certain that the UVette is placed in the same direction in the cuvette shaft for both the blank and the sample measurement. Because the self-absorbance values of the UVettes vary somewhat from one another, ideally, the same UVette should be used for both measurements. If changing the UVette when measuring a series of samples (recommendation: up to five measurements with one UVette) also the blank should be repeated using the new UVette.

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Eppendorf UVette^®

Scientist working with Eppendorf BioPhotometer and Uvette

Scientist putting cuvette into photometer from Eppendorf

Scientist measures sample with cuvette in photometer from Eppendorf

Scientist looking at cuvette from Eppendorf

Eppendorf UVette routine pack on table for many samples

Product Information

Working with very low amount of samples?
The UVette is a disposable UV/Vis cuvette with a light transmission of 220 nm to 1,600 nm for spectrophotometer and photometers. As an example of Eppendorf’s expertise in the production of high quality consumables, the UVette offers superb purity, convenience and precision for photometric analysis. The unique shape of the UVette combines two optical light path lengths to offer greater flexibility.

Show more Product Information

You will find additional download material at the bottom of this page

Product Information

Technical Data

Features

The Eppendorf UVette allows for measurements in the UV/Vis range with transparency from 220 nm to 1,600 nm with just a typical minimum volume of 50 µL. The UVette allows the user to choose between two optical path lengths (10 mm and 2 mm). The path length can be reduced by a factor of x5 without diluting or wasting any sample – simply by turning the UVette 90°. The UVette is suitable for fluorescence assays due to its low auto-fluorescence. The PCR-clean and protein-free individually sealed variant is ideal for accurate nucleic acid and protein quantification. The UVette is ideally matched for use with the Eppendorf BioPhotometer and Eppendorf BioSpectrometer families. The UVette can be used in other spectrophotometers with the aid of an adapter.
The UVettes are manufactured from crystal clear plastic, which is UV/Vis transparent and features translucency between 220 nm and 1,600 nm. The openly accessible side walls allow for a visual check of the liquid inside the UVette from all sides. This enables the user to detect air bubbles, for example, which may alter the test result. Furthermore, the funnel–shaped design of the cuvette base prevents a capillary effect and ensures that even the minimum volume of 50 µL constantly remains centered in the measuring area.
Two packaging sizes and purity grades are available:
• The individually packaged UVettes are PCR-clean & protein-free and are the ideal choice for valuable samples. Samples can be recovered for downstream applications without the risk of degradation or contamination. Lot specific certificates are available on the Eppendorf webpage.
• The routine pack contains UVettes with Eppendorf certified quality and is the ideal choice for routine applications where many samples need to be screened. It offers safe storage in a reclosable box and convenient access to each cuvette which makes filling easy.

For safe handling, cuvettes should be handled in combination with specific cuvette holders.

Selection guide for Eppendorf photometers

One cuvette - two path lengths

The unique shape of the UVette allows sample measurement using two different optical path lengths, which are clearly marked. A rotation of the UVette by 90° provides a choice between the 10 mm and the 2 mm optical path lengths. If the sample concentration is too high when measuring using the 10 mm light path, the UVette may simply be rotated by 90°, and the measurement can be repeated in the 2 mm light path.
On the other hand, if the sample concentration is lower than expected, one can easily switch from the 2 mm to the 10 mm light path. Thus the UVette provides great flexibility by covering measurements across a broad range of concentrations. Further information, e.g. about the chemical resistance of the UVette are available in our Knowledge Base.

Design

The UVettes are manufactured from crystal clear plastic, which is UV/Vis transparent and features translucency between 220 nm and 1,600 nm. The openly accessible side walls allow for a visual check of the liquid inside the UVette from all sides. This enables the user to detect air bubbles, for example, which may alter the test result. Furthermore, the funnel-shaped design of the cuvette base prevents a capillary effect and ensures that even the minimum volume of 50 µL constantly remains centered in the measuring area.

Two packaging sizes and purity grades are available:

• The individually packaged UVettes are PCR–clean & protein–free and are the ideal choice for valuable samples. Samples can be recovered for downstream applications without the risk of degradation or contamination. Lot specific certificates are available on the Eppendorf webpage.
• The routine pack contains UVettes with Eppendorf certified quality and is the ideal choice for routine applications where many samples need to be screened. It offers safe storage in a reclosable box and convenient access to each cuvette which makes filling easy.

Safe line-up of your cuvettes needed?

Due to their light weight and the small footprint, cuvettes tend to tip on the bench. To avoid this loss of high value samples due to these accidents, a dedicated rack for cuvettes is recommended. These racks have special square-footed wells for the cuvettes.
On top, the array of several cuvettes with individual sample content in a specific rack reduces the risk of mixing up your samples.

Read Less

Specifications	Eppendorf UVette^®

Volume range	50 – 2.000 µL
Purity grade	PCR clean
Packaging	80 pcs.
Dimensions (W × D × H)	12,5 × 12,5 × 36 mm / 0,49 × 0,49 × 1,42 in
Colorimetric protein assays	yes
Fluorescence measurements	yes
Light transmission	220 nm – 1,600 nm
Maximum filling volume	2000 µL
Height of light source	8.5 mm¹⁾
Use in Eppendorf BioPhotometer^®	yes
Use in Eppendorf BioSpectrometer^®	yes
OD 600 methods	yes
Temperature control	no
Wavelength range	220 – 1,600 nm
Sample light path length (mm)	2 mm and 10 mm
Cuvette blank	≤0.5 A at 260 nm
No. of optical windows	4
Material	UV-transparent plastic
dsDNA concentration range (UV 260 nm)	2.5 ng/µL – 750 ng/µL
BSA concentration range (UV 280 nm)	76 ng/µL – 22,725 ng/µL

Minimum filling volume
in Eppendorf photometers	50 µL
in devices of other manufacturers	50 µL

1)adapters available for height adjustment

Downloads: Eppendorf UVette^®

Flyer

Eppendorf Purity Grades Selection Guide

Spektrakuläre Performance – Eppendorf Photometer und Zubehör

PDF 9.2MB

German

Brochure

Spark of the Future - Sales stop

Spectracular performance - Eppendorf photometer and accessories

PDF 2.2MB

English

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