Influence of Physical Parameters on the Dispensed Volume of Air-Cushion Pipette

Beyond Science 04. August 2016

• Maintenance & Calibration
• Pipetting Techniques
• Essay
• Inspiring Science

The surrounding in the laboratory e.g., humidity, altitude and temperature, as well as the liquid properties of a sample itself like viscosity, volatility or density have a tremendous influence on the pipetting result. Each factor influences the pipetting volume and with this the experimental result in a different way. So a liquid temperature other than 22 °C already leads to less or excessive volume delivery. Or a dense liquid like phosphoric acid leads to constant insufficient liquid volume. All factors have to be considered during daily pipetting tasks and especially during calibration of a pipette. So consider our recommendations to improve your daily pipetting work.