How many parameters can I control using a DASGIP or DASbox system?

DASGIP Parallel Bioreactor Systems provide comprehensive concurrent monitoring and control of process parameters. During the process run, events are logged together with up to 70 online process variables per bioreactor. In addition to the various DASGIP modules for monitoring and control, with DASware analyze users can integrate analytical devices provided by third parties. Seamless integration into the DASGIP or DASbox system even allows for closed loop solutions such as automated feeding strategies based on the current glucose concentration. Each process value can be used as a trigger enabling users to automate their process.

How many parameters can be displayed in a process overview in DASware control?

DASware control users can freely select all relevant process variables of all or selected bioreactors to be displayed in one chart. There are no limitations in the number of process parameters. When using DASware analyze, the displayed process parameters even include integrated data from third party laboratory devices, such as nutrient analyzers, mass spectrometers or Raman. The software features tables and trend graphs of all measured values. It continuously logs and stores all data and events. These data are immediately accessible for evaluation using e.g. Microsoft Excel. Advanced charting functionalities ease further data analysis and comparison of runs.

Do I need programming skills to automate my process in DASware control?

DASware control includes a set of easy to implement automation features without any need of programming. Profiles and triggers allow for various control strategies, all of them easy to select by mouse click with parameters entered manually or just copy-pasted. Users with programming skills and special control requirements, however, are free to run scripted control strategies and profiles as well.

Can I change triggers, profiles, cascades and scripts online in DASware control?

Set-points, profiles, cascades, and scripts are online editable in DASware control. Offline values and events can be entered during or after the run and documented with a time stamp. These time stamps can be set individually, e.g to meet the time, when the sample was taken. This provides users with great flexibility to interact with the control system and reconsider parameter settings even in highly automated processes. All events, including online changes, are recorded in a SQL Server database.

How are DASGIP vessels agitated?

DASGIP bioreactors feature powerful overhead drives with agitation speeds ranging 30 – 1,600 rpm. The DASGIP TC4SC4 controls temperature and agitation in up to four bioreactors. Users can individually define each outlet set-point as well as choose from different operation modes (e.g. continuous or interval agitation).

Does DASware control give any alarms in case of critical deviation?

Critical deviation of process parameters such as pH, DO, redox, and temperature are color-coded in the software and saved in the event log. DASware control professional additionally allows notification of supervisory control systems or mobile communication tools via SMS or e-mail.

Can you help adjusting my controller loop parameters in DASware control?

DASGIP controllers are PI controllers, which are configured with default controller parameters adapted to the type of vessel and default experimental requirements. Depending on special customer requirements it might be necessary to modify these parameters. Our service will be happy to help you finding the right settings.

How to calibrate a pH sensor in DASware control?

DASware control software allows for parallel calibration of all sensors used within one system with all data logged in the central database together with set-points, profiles, cascades, process values, user interactions and other events. Good maintenance will prolong the sensors' lifetime whereas high temperatures (i.e. during autoclavation), alkaline solutions (i.e. during alkaline deactivation), repeated etchings and improper maintenance will reduce the electrode's lifetime.

How reliable are exhaust data when working at benchtop scale?

Exhaust oxygen and carbon dioxide concentrations of multiple bioreactor units are continuously monitored with the DASGIP GA exhaust analyzer. Together with the mass flow measurement and the input values of oxygen and carbon dioxide concentrations, automatic calculation of oxygen transfer rate (OTR), carbon dioxide transfer rate (CTR), and respiratory quotient (RQ) is possible, permitting direct conclusions on the metabolic state of a culture. As the DASGIP GA considers humidity, volume, and total flow typical sources for inaccurate OTR and CTR data in small working volumes are eliminated.

Is it possible to automatically control the foam in DASGIP vessels?

All DASGIP vessels can be equipped with foam control components. All you need is a level/foam sensor connected to the DASGIP sensor module (e.g. PH4PO4L) and a feed line for dosing of the antifoam agent. Now foam formation can be detected by the level sensor and antifoam agent is automatically pumped into the vessel until all foam is collapsed. The DASware control software allows any feed line to be used for antifoam or level control. Since medium composition has a strong impact on the electrical conductivity of medium and foam, every conductivity-based level sensor needs to be adjusted to foam and medium conductivity. DASware control supports monitoring conductivity and free adjustment of the conductivity threshold.

What maintenance is required for Eppendorf bioprocess systems?

As all complex technical systems Eppendorf bioprocess equipment should be maintained regularly to keep all parts in good condition. This maintenance avoids cost-intensive down times and contributes to preservation of value. A complete preventive maintenance is recommended once a year. Additional maintenance actions are recommended to be executed by the user prior to every run or in regular cycles (e.g. every month). Our service will be happy to advice.

What do I have to consider when returning equipment for repair?

Equipment returned to Eppendorf for service or any other reason needs to be decontaminated first. Please fill out the Decontamination Form that you will find in the “Service & Support” section of the Eppendorf website and attach it to the package. Equipment which was sent without the completely filled form cannot be handled. This form must also be completed before any on-site service, whether covered under warranty or not, can commence.

What does your warranty cover?

Eppendorf issues a warranty of one year from the day of delivery on its products in accordance with our terms and conditions. Excluded are consumables such as cables, tubing, glassware, and sensors as well as used material.

Does Eppendorf offer bioreactor training?

We highly recommend a training at the customer’s site for all new customers. Besides this initial training, Eppendorf also offers individual trainings adapted to the user’s requirements like training of new employees or advanced training/recapitulation for employees already having experience with DASGIP or DASbox systems. All trainings are performed at the customer’s site using the customer’s equipment to have the training focused on the customer’s demands. The structure and methods of the training can be chosen to the customer’s requirements from general overview to detailed sessions on specific products or issues. Training in small groups is as possible as personal guidance.

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DASbox^® Mini Bioreactor System

BioBLU c and BioBLU f Single-Use Bioreactors for small and bench scale cell culture, stem cell, and fermentation applications.

Produktinformationen

The DASbox Mini Bioreactor System is a parallel bioreactor system for research and process development in small working volumes. It can operate up to 24 glass or BioBLU^® Single-Use Bioreactors in parallel and is suitable for microbial fermentation and cell culture, including the cultivation of stem cells. With working volumes from 60 mL to 250 mL it is an optimal tool for efficient process development, Design of Experiments (DoE) approaches, and scale-down applications.

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Eigenschaften

Small scale. Big results.

The DASbox Mini Bioreactor System enables highly efficient process development by allowing precise control of critical process parameters, rapid comparison of numerous experimental conditions, reduction of medium costs, and optimal utilization of all generated data.
• Small working volumes save resources.
• Parallel operation of up to 24 bioreactors saves time and space and improves reproducibility.
• Easily convertible from single-use to reusable bioreactors and vice versa.
• DASware^® software suite enables precise monitoring and control of critical process parameters, as well as the implementation of tailored feedback loops, feeding strategies and other features.
• Seamless integration with the Bioprocess Autosampler allows for automated sampling 24/7 to gain complete datasets.
• Connects to BioNsight^® cloud software for remote process monitoring and simple, remote analysis of all data sets to make informed decisions.

Operate small bioreactors in parallel

• The DASbox Mini Bioreactor System is designed as a 4-fold system that is expandable to up to 24 parallel operating bioreactors.
• A 24-fold DASbox system takes up only 1.8 m (6 ft) of bench space. This corresponds to 7.5 cm (3 in) per vessel.
• The setpoints, control strategies, and inoculation time of each bioreactor can be controlled individually from one software installation. Like this, multiple experimental conditions can be tested in parallel, for example in a Design of Experiments (DoE) approach.

Automate bioprocess sampling

• The DASbox system can be seamlessly integrated with the Bioprocess Autosampler, which takes samples from multiple bioreactors 24/7 in short and regular intervals and stores them tempered for later analysis.
• The Bioprocess Autosampler can be installed with new as well as retrofitted to existing bioreactor control systems.

Cultivate various cell types, including stem cells

• The DASbox is a unique mini bioreactor system suitable for microbial and cell culture as well as stem cell applications.
• Glass and single-use bioreactor variants are available, which are optimized to support the special needs of stem cells, such as low-shear mixing and aggregate formation.

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Spezifikationen	DASbox^® Zellkultur	DASbox^® Zellkultur, Einweg	DASbox^® Mikrobiologie	DASbox^® Mikrobiologie, Einweg

Anwendung	Zellkultur	Zellkultur	Mikrobiologie	Mikrobiologie
Anzahl paralleler Einheiten	bis zu 24	bis zu 24	bis zu 24	bis zu 24
Software	DASware control, andere DASware optional erhältlich	DASware control, andere DASware optional erhältlich	DASware control, andere DASware optional erhältlich	DASware control, andere DASware optional erhältlich
Benutzeroberfläche	Prozessrechner mit Monitor	Prozessrechner mit Monitor	Prozessrechner mit Monitor	Prozessrechner mit Monitor
Typische Leistungsaufnahme (4-fach-System ohne Prozessrechner)	168 W (bei 230 V)/ 154 W (bei 115 V)	168 W (bei 230 V)/ 154 W (bei 115 V)	168 W (bei 230 V)/ 154 W (bei 115 V)	168 W (bei 230 V)/ 154 W (bei 115 V)
Abmessungen (B × T × H, 4-fach-System ohne Prozessrechner)	30 x 70 x 49 cm (12 x 28 x 19 in)	30 x 70 x 49 cm (12 x 28 x 19 in)	30 x 70 x 49 cm (12 x 28 x 19 in)	30 x 70 x 49 cm (12 x 28 x 19 in)
Produktgewicht	44 kg	44 kg	44 kg	44 kg
Typisches Gewicht	44 kg	44 kg	44 kg	44 kg
Bakterien/Hefen/Pilze	ja	ja	ja	ja
Säuger-/tierische Zellen	ja	ja	ja	ja
Insektenzellen	ja	ja	ja	ja
Austauschbare Gefäße	ja	ja	ja	ja
Validierbar	nein	nein	nein	nein
Stammzellen	ja	ja	ja	ja

Bioreaktoren
Gefäße	Glasgefäße	Einweggefäße	Glasgefäße	Einweggefäße
Sterilisation	Autoklavierbar	Vorsterilisiert	Autoklavierbar	Vorsterilisiert
Gesamtvolumen	350 mL	380 mL	350 mL	380 mL

Rührer
Antrieb	direkte Überkopfantriebe	Überkopfmagnetantriebe	direkte Überkopfantriebe	Überkopfmagnetantriebe
Drehzahlbereiche	20 – 2500 rpm	20 – 500 rpm	20 – 2500 rpm	20 – 2000 rpm
Rührer	Propellerrührer	Schrägblattrührer	Rushton-Typ-Rührer	Rushton-Typ-Rührer

Begasung
Gasversorgung	TMFC; Sparger und/oder Kopfraumbegasung	TMFC; Sparger und/oder Kopfraumbegasung	TMFC; Sparger	TMFC; Sparger
Standardbegasungsraten	0,04 – 5 sL/h, 0,04 – 3,5 sL/h CO₂	0,04 – 5 sL/h, 0,04 – 3,5 sL/h CO₂	0,2 – 25 sL/h, 0,2 – 18 sL/h CO₂	0,2 – 25 sL/h, 0,2 – 18 sL/h CO₂
Standard-Gasmischung	Luft, N₂, O₂ und/oder CO₂	Luft, N₂, O₂ und/oder CO₂	Luft, N₂, O₂ und/oder CO₂	Luft, N₂, O₂ und/oder CO₂

Feeding
Dosierleitungen pro Gefäß	2 (Standard)/ 4 (optional)	2 (Standard)/ 4 (optional)	2 (Standard)/ 4 (optional)	2 (Standard)/ 4 (optional)
Standarddosierraten (abhängig vom Gefäßdurchmesser)	0,3 – 9,5 mL/h bis 13 – 420 mL/h	0,3 – 9,5 mL/h bis 13 – 420 mL/h	0,3 – 9,5 mL/h bis 13 – 420 mL/h	0,3 – 9,5 mL/h bis 13 – 420 mL/h

Überwachung und Steuerung
Temperaturregelung	Flüssigkeitsfreies Heizen und Kühlen (Peltier)	Flüssigkeitsfreies Heizen und Kühlen (Peltier)	Flüssigkeitsfreies Heizen und Kühlen (Peltier)	Flüssigkeitsfreies Heizen und Kühlen (Peltier)
Standardtemperaturbereich (bei 25 °C Umgebungstemp.)	10 – 60 °C	10 – 45 °C	10 – 60 °C	10 – 45 °C
pH-Regelung	CO₂/Base und andere Set-ups	CO₂/Base und andere Set-ups	Säure und/oder Base und andere Set-ups	Säure und/oder Base und andere Set-ups
DO-Regelung	Kaskade (O₂-Konzentration, Begasungsrate) und andere Set-ups	Kaskade (O₂-Konzentration, Begasungsrate) und andere Set-ups	Kaskade (Rührgeschwindigkeit, O₂-Konzentration, Begasungsrate) und andere Set-ups	Kaskade (Rührgeschwindigkeit, O₂-Konzentration, Begasungsrate) und andere Set-ups
ORP-Messung (Redox)	-	-	Optional (Redox oder Level wählen)	Optional (Redox oder Level wählen)
Level/Schaum	Optional	Optional	Optional (Redox oder Level wählen)	Optional (Redox oder Level wählen)
OD-Messung	Optional (DASGIP OD4)	Optional (DASGIP OD4)	Optional (DASGIP OD4)	Optional (DASGIP OD4)
Abgaskondensation	flüssigkeitsfrei (Peltier)	flüssigkeitsfrei (Peltier)	flüssigkeitsfrei (Peltier)	flüssigkeitsfrei (Peltier)
Abgasanalyse	-	-	Optional (DASGIP GA4)	Optional (DASGIP GA4)

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