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Eppendorf twin.tec® real-time PCR Plates - qPCR Plates

Product Information

The white wells of Eppendorf’s twin.tec® real-time PCR plates increase the reflection of fluorescent light in real-time PCR (qPCR) and other fluorescence assays. The increased fluorescence from these specialized qPCR plates improves the sensitivity of your assays, while also improving reproducibility and consistency of your results.

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Products (11)

Accessories (1)


Save 50% on Eppendorf twin.tec® real-time PCR plates
    • unskirted, low profile, 150 µL
    • PCR clean, white, includes 2× sets of 20 plates
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    Quantity
    Catalog no. 2231001129
    Price
    $ 183.00
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    Save 50% on Eppendorf twin.tec® real-time PCR plates
      • semi-skirted, 250 µL
      • PCR clean, blue, includes 2× sets of 25 plates
      More Product Details ...
      Quantity
      Catalog no. 2231001130
      Price
      $ 242.00
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      Save 50% on Eppendorf twin.tec® real-time PCR plates
        • skirted, 150 µL
        • PCR clean, white, includes 2× sets of 25 plates
        More Product Details ...
        Quantity
        Catalog no. 2231001131
        Price
        $ 242.00
        Inquire

          • semi-skirted, 250 µL
          • Forensic DNA Grade, white, 10 plates, individually wrapped
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          Quantity
          Catalog no. 0030129644
          Price
          $ 123.00
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            • unskirted, low profile, 150 µL
            • PCR clean, white, 20 plates
            More Product Details ...
            Quantity
            Catalog no. 0030132700
            Price
            $ 183.00
            Inquire

              • unskirted, low profile, 150 µL
              • PCR clean, blue, 20 plates
              More Product Details ...
              Quantity
              Catalog no. 0030132718
              Price
              $ 183.00
              Inquire

                • semi-skirted, 250 µL
                • PCR clean, blue, 25 plates
                More Product Details ...
                Quantity
                Catalog no. 951022043
                Price
                $ 242.00
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                  • skirted, 150 µL
                  • PCR clean, blue, 25 plates
                  More Product Details ...
                  Quantity
                  Catalog no. 951022003
                  Price
                  $ 258.00
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                    • semi-skirted, 250 µL
                    • PCR clean, white, 25 plates
                    More Product Details ...
                    Quantity
                    Catalog no. 951022055
                    Price
                    $ 242.00
                    Inquire

                      • skirted, 150 µL
                      • Forensic DNA Grade, white, 10 plates, individually wrapped
                      More Product Details ...
                      Quantity
                      Catalog no. 0030129636
                      Price
                      $ 123.00
                      Inquire

                      Product Information

                      Applications

                      Features

                      The limiting factor in low-volume real-time PCR (qPCR) is often the remaining fluorescence intensity. Eppendorf twin.tec real-time PCR plates are specially manufactured for qPCR, as they offer all the advantages of regular twin.tec plates, but with the added advantage of white wells. These reflect fluorescence much better than clear or frosted wells, so lower levels of fluorescence are still detectable with the same instrument – just by using the right consumables. The qPCR plates' white wells also significantly reduce interfering background fluorescence and lead to increased consistency between replicates, and giving highly reproducible results.

                      The wells are made from polypropylene – which guarantees fast heat transfer for fast assays – and the frame is made of rigid polycarbonate, to ensure easy and reliable handling – both in manual and automated assays.
                      High fluorescence intensity is instrumental in improving sensitivity in qPCR. This graph shows an up to 10-fold higher fluorescence intensity in twin.tec® real-time PCR plates compared to frosted wells.
                      Eppendorf twin.tec® real-time PCR plates reduce Ct values more effectively than other tested plates. On average, Ct values were 0.8 cycles lower (compared to clear wells), indicating better sensitivity.
                      White wells improve homogeneity of results over the block. In this graph you can see the reduced standard deviation over the block compared to other plates.