Is the Varispenser resistant to morpholine (tetrahydro-1,4-oxazine)?

Yes. Due to the hygroscopic properties of morpholine, please use the drying tube (4960 851.00). The drying tube should be filled with dried calcium carbonate.

Is it possible to dispense crude oil using Varispenser?

The Varispenser is chemically resistant to crude oil. Difficulties may be caused by the viscosity, depending on the composition and temperature of the crude oil. The Varispenser is intended for dispensing liquids with a maximum kinematic viscosity of 500 mm2/s . It is thus necessary to work extremely slowly, and more effort is required on the part of the user. Furthermore blockage of the Varispenser hoses and valves is observed very fast, thus an immediate cleaning is necessary.

Can the Varispenser be used to dispense methyl butyl ether?

Yes, the Varispenser can be used for this. Methyl butyl ether is highly flammable! Warning: follow safety information!

Can the Varispenser be used to dispense dibutylamine?

Dibutylamine is very similar to ammonia. The Varispenser is resistant to dibutylamine.

Can 0.1 n butylamine and ethyl acetate be used in the Varispenser?

Both 0,1 N butylamine and ethyl acetate were tested and can be used.

During the dispensing process, does the liquid come into contact with any metal components of the Varispenser?

Yes. The spring system of the spring-loaded platinum-iridium valve ball comes into contact with the liquid during dispensing.

I would like to distribute liquid agar onto petri dishes with the Varispenser. How hot can the agar be for this?

Varispensers are measurement devices for which the guaranteed accuracy, precision or error limits only apply in a restricted temperature range of 15 - 40 °C (conforming to DIN 12650). Applications with liquids outside this temperature range are not recoommended and can only be carried out at the user's own risk. At higher temperatures up to 60 °C the plastic materials fluorocarbon and polypropylene will be fully chemically resistant, but mechanically they will become slightly softer. When hot agar is aspirated out of the bottle, cooling will take place in the cold Varispenser. Since agar becomes much firmer at ca. 35 °C, blockage can easily occur. In any case immediate cleaning will be required, in particular of the valve.

Is it possible to use the Varispenser to dispense agarose gels at approx. 90 °C?

Varispensers are measurement devices for which the guaranteed accuracy, precision or error limits only apply in a restricted temperature range of 15 - 40 °C (conforming to DIN 12650). Heating the device to 90°C is not permitted and can only be carried out at the user's own risk.

Is it possible to autoclave the Varispenser attached to a full flask?

Yes, this is possible. Please mind the following precautions: The Varispenser is loosely screwed on the bottle, the volume setting knob is set to 20% of the nominal volume and the piston is completely pulled out.

Is it possible to autoclave the Varispenser 2(x) attached to a bottle?

No, it is not possible to autoclave the Varispenser 2/x attached to a bottle. The device must be placed horizontal on a cloth in the autoclave (according to operating manual chapter 5.9).

Is it possible to exchange the dispensing tube of the Varispenser 2 and 2x among each other?

Yes, it is possible to exchange the dispensing tube of the Varispenser 2 with a dispensing tube of the Varispenser 2x. Please note that the device is then capable of recirculating the liquid back into the bottle.

Do aspiration tubes of different lengths exist for Varispenser 2(x)?

Yes, aspiration tubes of different lengths are available to cover most bottle types.

Does the aspiration tube of the Varispenser 2(x) need to be cut diagonal for aspiration?

No, the aspiration tube of Varispenser 2(x) does not need to be cut diagonal, because two holes on the bottom side of the tube enable liquid uptake.

What is the "flexible discharge tube" used for?

The "flexible discharge tube" can be used to reach into small or narrow-standing tubes without tilting the botlle. More Information are available in the White Paper 38 .

For which liquids a drying tube is beneficial?

The drying tube should be used when working with fuming acids to avoid dilution. It can tehn be filled with e.g., calcium chloride or silica pills. When working with alkaline solutions filling of the drying tube with sodium hydroxide pills prevents carbonate formation and crystallization of the liquid. More Information are available in the White Paper 38 .

Is it possible to dispense sterile medium with the Varispenser 2(x)?

Yes, it is possible to dispense sterile medium with the Varispenser 2(x). We recommend the usage of a sterile filter at the back of the device to keep the sterility of the medium. More Information are available in the White Paper 38 .

Is it possible to adjust the Varispenser 2(x)?

Yes, it is possible to adjust the Varispenser 2(x) for liquids with different properties than water. Please refer to the operating manual for further information.

How can the Varispenser 2(x) be decontaminated?

The outer of the Varispenser 2/x can be wiped with lint-free tissue and 70 % ethanol. When sterilization of the inner parts is needed, the Varispenser 2/x can be autoclaved. The dispenser does not need to be dissembled for autoclaving, but it must be placed horizontal on a cloth in the autoclave (according to operating manual chapter 5.9).

Is the Varispenser 2/2x suited for dispensing 100% acetic acid (glacial acetic acid)?

Yes. 100% acetic acid can be dispensed with the Varispenser 2/2x. When using concentrated acids the usage of a drying tube is furthermore highly recommended. More information about the chemical resistance of the Varispenser 2/2x is available here .

Can the Varispenser 2/2x be used to dispense a conc. sulfuric acid which contains potassium bichromate and silver sulfate?

Yes, you can use the Varispenser 2/2x for this application. Please notice, when using concentrated acids a drying tube should be connected to the Varispenser. More information about the chemical resistance of the Varispenser 2/2x is available here .

Which physical limits does the Varispenser 2/2x has?

The device is intended for dispensing liquids with following properties: ambient temperature 15°C - 40°C , maximum density: 2.2 g/cm3, maximum vapor pressure: 500 mbar. Above 300 mbar slow aspiration is recommended to avoid boiling., kinematic viscosity: 500 mm2/s. More details are written in the operating manual .

I bought a new Varispenser 2(x) and drops are visible inside the device. Additionally an intense smell was present when opening the cardbox. What is that?

Each Varispenser 2(x) is calibrated before packaging using distilled water. Afterwards each device is rinsed with an ethanol-glycerol solution to dry faster. The drops inside the device and the smell occur from this solution. The device should be completely dry after a few minutes outside the packaging.

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Varispenser^® 2/ Varispenser^® 2x - Bottle-Top Dispenser

The Varispenser 2(x) from Eppendorf is available in six different volume sizes

Eppendorf Varispenser_REG_ 2 and Varispenser_REG_ 2x bottle-top dispensers – six different sizes for dispensing volumes from 0.2 – 100 mL

Lab technician using the Eppendorf Varispenser_REG_ 2x bottle-top dispenser

Easily fill Conical Tubes with the Varispenser_REG_ 2(x) bottle-top dispenser from Eppendorf

Lab technician using the Eppendorf Varispenser_REG_ 2x bottle-top dispenser to fill a glass vial

The Varispenser_REG_ 2x bottle-top dispenser's recirculation valve lever

The Varispenser_REG_ 2(x) bottle-top dispensers feature an easy-to-read volume scale

Varispenser_REG_ 2/2x bottle-top dispenser ventilation screw with filter

Varispenser_REG_ 2 bottle-top dispensers from Eppendorf are resistant to many chemicals and autoclavable

Varispenser_REG_ 2x bottle-top dispensers with the Eppendorf Top Buret

Varispenser_REG_ 2 and 2x bottle-top dispensers from Eppendorf fit on a variety of supply bottles and are chemical-resistant

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Product Information

Need a bottle-top dispenser for aggressive solutions such as acids, solutions or bases? The Eppendorf bottle-top dispensers Varispenser 2 and Varispenser 2x allow you to safely dispense liquids from a wide range of bottles without reagent waste and are ideal for conveniently dispensing aliquots from large supply bottles. Chemically resistant and autoclavable, their tight seal provides protection against caustic media.

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You will find additional download material at the bottom of this page

Product Information

Technical Data

Applications

Features

Safe and easy liquid dispensing from bottles without compromise

Master difficult liquid dispensing tasks with the Varispenser 2 and Varispenser 2x universally applicable bottle-top dispensers. Available in six different sizes for liquid dispensing of volumes from 0.2 – 100 mL, the Varispenser 2 and 2x are designed to consistently dispense selected volumes with no reagent waste. The unique PFA sealing lip technology prevents crystal building from media residue by wiping off the inner cylinder wall during dispensing. This technology, combined with their high chemical resistance, allows the Varispenser 2 and 2x to be used for nearly all kinds of liquids, including aggressive solutions like acids, bases or solvents.
A special thread design and additional thread adapters ensure their use with most large supply and reagent bottles in the laboratory, while an extra air vent cap helps prevent possible contamination from the supply bottle. With a 360° rotating valve block offering safe and easy access in all positions, Varispenser 2 and Varispenser 2x really do make aliquoting liquids safe and convenient.
The Varispenser 2x has an additional recirculation valve which prevents reagent loss while ventilating. Both variants are fully autoclavable.

Wide choice of accessories for all your large volume dispensing needs

A variety of accessories are available for the Varispenser 2 and Varispenser 2x bottle-top dispensers to help you overcome any liquid dispensing challenge you might meet in the lab. Choose from a selection of drying tubes, filters, flexible discharge tubes, ETFE bottle thread adapters and more to speed up your workflows and prevent contamination. Choosing the right accessory for your bottle-top dispenser...

• ... speeds up your workflow
• ... reduces the risk of contamination
• ... can help reduce plastic waste: Disposable consumables, such as pipette tips for liquid transfer, are not needed

Read more on the right accessories for your bottle-top dispenser in our White Paper No. 38.

Your bottle-top dispenser choice when working with aggressive chemicals

Whether you are using corrosive, inflammable, high viscosity or volatile liquids, the built-in safety features of Varispenser 2 or Varispenser 2x protect you from accidental spills and ensure smooth and effortless operation. Their high chemical resistance means these bottle-top dispensers can be used with a variety of liquid reagents in a range of applications often found in biological, pharmaceutical, chemistry or forensic labs - all while providing superior sealing over a wide range of temperatures and viscosities. Examples include:

• Corrosive and inflammable liquids
• Liquids of high viscosity
• Detergent solutions
• Foaming liquids
• Liquids with high vapor pressure or density

If you need to dispense fuming acids, which are often used in the chemical industry, it’s important to ensure they are kept free from moisture. A drying tube filled with a moisture absorber is the ideal accessory for this application.

How to use a Varispenser 2 bottle-top dispenser

Attach the telescopic aspirating tube and recirculation valve (Varispenser 2x only) to the bottle-top dispenser before attaching it to your supply bottle using the appropriate adapter. Unlock the volume selection slider by pushing it to the right, slide it to your desired volume and lock it by pushing the slider to the left. Vent the dispenser by pulling up the piston and pushing it down to dispense liquid into any target vessel until the liquid inside the cylinder is free from air bubbles. Dispense liquid by following the same steps after venting. Note that the dispenser is not a suitable bottle closure and should be removed from the bottle and properly cleaned after use. Learn more in our tutorial videos and by reading the operating manual.

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Specifications	Varispenser^® 2x - 4967000014	Varispenser^® 2 - 4966000010	Varispenser^® 2x - 4967000022	Varispenser^® 2 - 4966000029	Varispenser^® 2x - 4967000030	Varispenser^® 2 - 4966000037	Varispenser^® 2x - 4967000049	Varispenser^® 2 - 4966000045	Varispenser^® 2x - 4967000057	Varispenser^® 2 - 4966000053	Varispenser^® 2x - 4967000065	Varispenser^® 2 - 4966000061

Product type	Dispensers	Dispensers	Dispensers	Dispensers	Dispensers	Dispensers	Dispensers	Dispensers	Dispensers	Dispensers	Dispensers	Dispensers
Pipetting type	Positive displacement system	Positive displacement system	Positive displacement system	Positive displacement system	Positive displacement system	Positive displacement system	Positive displacement system	Positive displacement system	Positive displacement system	Positive displacement system	Positive displacement system	Positive displacement system
Number of channels	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel
Volume range	0.2 – 2 mL	0.2 – 2 mL	0.5 – 5 mL	0.5 – 5 mL	1 – 10 mL	1 – 10 mL	2.5 – 25 mL	2.5 – 25 mL	5 – 50 mL	5 – 50 mL	10 – 100 mL	10 – 100 mL
Volume increment	0.05 mL	0.05 mL	0.1 mL	0.1 mL	0.2 mL	0.2 mL	0.5 mL	0.5 mL	1 mL	1 mL	1 mL	1 mL
Operating mode	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical
Random measurement error	0.2 mL: ± 1%; ± 2 µL 1 mL: ± 0.2%; ± 2 µL 2 mL: ± 0.1%; ± 2 µL	0.2 mL: ± 1%; ± 2 µL 1 mL: ± 0.2%; ± 2 µL 2 mL: ± 0.1%; ± 2 µL	0.5 mL: ± 1%; ± 5 µL 2.5 mL: ± 0.2%; ± 5 µL 5 mL: ± 0.1%; ± 5 µL	0.5 mL: ± 1%; ± 5 µL 2.5 mL: ± 0.2%; ± 5 µL 5 mL: ± 0.1%; ± 5 µL	1 mL: ± 1%; ± 10 µL 5 mL: ± 0.2%; ± 10 µL 10 mL: ± 0.1%; ± 10 µL	1 mL: ± 1%; ± 10 µL 5 mL: ± 0.2%; ± 10 µL 10 mL: ± 0.1%; ± 10 µL	2.5 mL: ± 1%; ± 25 µL 12.5 mL: ± 0.2%; ± 25 µL 25 mL: ± 0.1%; ± 25 µL	2.5 mL: ± 1%; ± 25 µL 12.5 mL: ± 0.2%; ± 25 µL 25 mL: ± 0.1%; ± 25 µL	5 mL: ± 1%; ± 50 µL 25 mL: ± 0.2%; ± 50 µL 50 mL: ± 0.1%; ± 50 µL	5 mL: ± 1%; ± 50 µL 25 mL: ± 0.2%; ± 50 µL 50 mL: ± 0.1%; ± 50 µL	10 mL: ± 1%; ± 100 µL 50 mL: ± 0.2%; ± 100 µL 100 mL: ± 0.1%; ± 100 µL	10 mL: ± 1%; ± 100 µL 50 mL: ± 0.2%; ± 100 µL 100 mL: ± 0.1%; ± 100 µL
Systematic measurement error	0.2 mL: ± 5%; ± 10 µL 1 mL: ± 1%; ± 10 µL 2 mL: ± 0.5%; ± 10 µL	0.2 mL: ± 5%; ± 10 µL 1 mL: ± 1%; ± 10 µL 2 mL: ± 0.5%; ± 10 µL	0.5 mL: ± 5%; ± 25 µL 2.5 mL: ± 1%; ± 25 µL 5 mL: ± 0.5%; ± 25 µL	0.5 mL: ± 5%; ± 25 µL 2.5 mL: ± 1%; ± 25 µL 5 mL: ± 0.5%; ± 25 µL	1 mL: ± 5%; ± 50 µL 5 mL: ± 1%; ± 50 µL 10 mL: ± 0.5%; ± 50 µL	1 mL: ± 5%; ± 50 µL 5 mL: ± 1%; ± 50 µL 10 mL: ± 0.5%; ± 50 µL	2.5 mL: ± 5%; ± 125 µL 12.5 mL: ± 1%; ± 125 µL 25 mL: ± 0.5%; ± 125 µL	2.5 mL: ± 5%; ± 125 µL 12.5 mL: ± 1%; ± 125 µL 25 mL: ± 0.5%; ± 125 µL	5 mL: ± 5%; ± 250 µL 25 mL: ± 1%; ± 250 µL 50 mL: ± 0.5%; ± 250 µL	5 mL: ± 5%; ± 250 µL 25 mL: ± 1%; ± 250 µL 50 mL: ± 0.5%; ± 250 µL	10 mL: ± 5%; ± 500 µL 50 mL: ± 1%; ± 500 µL 100 mL: ± 0.5%; ± 500 µL	10 mL: ± 5%; ± 500 µL 50 mL: ± 1%; ± 500 µL 100 mL: ± 0.5%; ± 500 µL
Autoclavable	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes
Open system (third party consumables)	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes
Thread	GL 45	GL 45	GL 45	GL 45	GL 45	GL 45	GL 45	GL 45	GL 45	GL 45	GL 45	GL 45
Bottle thread adapter incl.	GL 25, GL 28/S 28, GL 32, GL 38, S 40¹⁾¹⁾	GL 25, GL 28/S 28, GL 32, GL 38, S 40¹⁾¹⁾	GL 25, GL 28/S 28, GL 32, GL 38, S 40¹⁾¹⁾	GL 25, GL 28/S 28, GL 32, GL 38, S 40¹⁾¹⁾	GL 25, GL 28/S 28, GL 32, GL 38, S 40¹⁾¹⁾	GL 25, GL 28/S 28, GL 32, GL 38, S 40¹⁾¹⁾	GL 32, GL 38, S 40¹⁾	GL 32, GL 38, S 40¹⁾	GL 32, GL 38, S 40¹⁾	GL 32, GL 38, S 40¹⁾	GL 32, GL 38, S 40¹⁾	GL 32, GL 38, S 40¹⁾
Delivery content	Dispenser, Operating manual, Certificate, Telescopic aspirating tube (125 mm - 240 mm), Thread adapter (x5: 25 mm, 28 mm, 32 mm, 38 mm, 40 mm), Universal wrench	Dispenser, Operating manual, Certificate, Telescopic aspirating tube (125 mm - 240 mm), Thread adapter (x5: 25 mm, 28 mm, 32 mm, 38 mm, 40 mm), Universal wrench	Dispenser, Operating manual, Certificate, Telescopic aspirating tube (125 mm - 240 mm), Thread adapter (x5: 25 mm, 28 mm, 32 mm, 38 mm, 40 mm), Universal wrench	Dispenser, Operating manual, Certificate, Telescopic aspirating tube (125 mm - 240 mm), Thread adapter (x5: 25 mm, 28 mm, 32 mm, 38 mm, 40 mm), Universal wrench	Dispenser, Operating manual, Certificate, Telescopic aspirating tube (125 mm - 240 mm), Thread adapter (x5: 25 mm, 28 mm, 32 mm, 38 mm, 40 mm), Universal wrench	Dispenser, Operating manual, Certificate, Telescopic aspirating tube (125 mm - 240 mm), Thread adapter (x5: 25 mm, 28 mm, 32 mm, 38 mm, 40 mm), Universal wrench	Dispenser, Operating manual, Certificate, Telescopic aspirating tube (170 mm - 330 mm), Thread adapter (x3: 32 mm, 38 mm, 40 mm), Universal wrench	Dispenser, Operating manual, Certificate, Telescopic aspirating tube (170 mm - 330 mm), Thread adapter (x3: 32 mm, 38 mm, 40 mm), Universal wrench	Dispenser, Operating manual, Certificate, Telescopic aspirating tube (170 mm - 330 mm), Thread adapter (x3: 32 mm, 38 mm, 40 mm), Universal wrench	Dispenser, Operating manual, Certificate, Telescopic aspirating tube (170 mm - 330 mm), Thread adapter (x3: 32 mm, 38 mm, 40 mm), Universal wrench	Dispenser, Operating manual, Certificate, Telescopic aspirating tube (170 mm - 330 mm), Thread adapter (x3: 32 mm, 38 mm, 40 mm), Universal wrench	Dispenser, Operating manual, Certificate, Telescopic aspirating tube (170 mm - 330 mm), Thread adapter (x3: 32 mm, 38 mm, 40 mm), Universal wrench

1)buttress thread

Downloads: Varispenser^® 2/ Varispenser^® 2x

Selection Guide

Get It Right! Selection Chart for Liquid Handling Instruments & Tips

Find the most suitable pipetting system for your lab applications

You give your all to scientific research every day. Eppendorf liquid handling instruments help you grow beyond your limits

PDF 8.3MB

English

Brochure

Pipette Service Done Right

Eppendorf Pipette Calibration

Uncompromising from the bottom to the top –
Varispenser^® 2 and Varispenser^® 2x bottle-top dispensers

PDF 1.0MB

English

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Varispenser® 2/ Varispenser® 2x - Bottle-Top Dispenser

The Varispenser 2(x) from Eppendorf is available in six different volume sizes

Eppendorf Varispenser® 2 and Varispenser® 2x bottle-top dispensers – six different sizes for dispensing volumes from 0.2 – 100 mL

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The Varispenser® 2(x) bottle-top dispensers feature an easy-to-read volume scale

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Varispenser® 2 bottle-top dispensers from Eppendorf are resistant to many chemicals and autoclavable

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Product Information

Varispenser® 2(x) Bottle-Top Dispenser

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Product Information

Safe and easy liquid dispensing from bottles without compromise

Wide choice of accessories for all your large volume dispensing needs

Your bottle-top dispenser choice when working with aggressive chemicals

How to use a Varispenser 2 bottle-top dispenser

Technical Data

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