How do I determine the volume values when calibrating gravimetrically?

The measuring values of the analysis scales are multiplied by the correction factor Z (see SOP pipettes). This takes into account the density of the water at the relevant temperature and the relevant air.

What does EN ISO 8655 cover?

ISO 8655 addresses the needs of suppliers, test houses, and users and specifies the general requirements for piston-operated liquid handling devices. It is structured in seven chapters: ISO 8655-1: Terminology, general requirements, and user recommendations ISO 8655-2: Piston pipettes ISO 8655-3: Piston burettes ISO 8655-4: Dilutors ISO 8655-5: Dispensers ISO 8655-6: Gravimetric method for the determination of measurement error ISO 8655-7: Non-gravimetric methods for assessment of equipment performance (titration, photometry)

What do the terms random error and systematic error mean in a pipette calibration?

The systematic error describes the deviation of the dispensed volume from the nominal volume or from the selected volume of the piston stroke device, and is determined by establishing the mean value of (usually) 10 measurements. The random error describes the mean variation of the dispensed volumes from the mean value of the dispensed volumes, and is determined by calculating the repeat standard deviation of (usually) 10 measurements.

Why are the Z factors listed in our SOP different from factors which can be derived from density reference values (of water)?

The Z factor is included in EN ISO 8655-6 and was incorporated into our SOP. As we understand it, it was incorporated in the standard by the Physikalisch Technische Bundesanstalt in Braunschweig. The Z factor describes the density of water at a particular temperature using a 5th degree polynomial. Furthermore, the density of water is corrected by the density of air (derived from air pressure) and a fixed correction value for balances. These corrections are the key reasons for the deviation from density values in various books of tables.

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Varipette^® 4720 - Large Volume Pipette

Varipette_REG__NBSP_4720 positive displacement pipette from Eppendorf

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Handle challenging liquid handling tasks with ease! The Varipette 4720 is a large volume, positive displacement pipette that is optimized for lab work with viscous or volatile liquids. Ideal for safely aspirating liquids from large bottles or tall, narrow vessels.

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The large volume positive displacement pipette for large bottles or tall, narrow vessels

Pipette faster and enjoy convenient one-handed operation with the Varipette 4720 large volume positive displacement pipette from Eppendorf. Optimized for work with viscous or volatile, high-vapor pressure liquids, the Varipette 4720 pipette is continuously adjustable and designed to aspirate from large bottles, or tall, narrow vessels when using the Eppendorf Varitips^® S system or from beakers using the Varitip P pipette tips.

Super easy, one-handed operation significantly speeds up pipetting tasks – simply press down the Varipette operating lever to aspirate liquid from the reservoir; press the lever again and the liquid will be dispensed into your sample vessel.

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Varipette® 4720 - Large Volume Pipette

Varipette® 4720 positive displacement pipette from Eppendorf

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