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DNA LoBind® Plates
Eppendorf LoBind® - How it works
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Product Information
Our innovative DNA LoBind technology reduces sample-to-surface binding to ensure maximum nucleic acid recovery and offer the ideal solution for sample preparations and long-term storage.
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Applications
Features
Product Information
Maximize recovery of nucleic acids with DNA LoBind® Plates! A combination of special manufacturing technologies and selected polypropylene batches ensure nearly 100 % recovery of DNA/RNA molecules-without surface coating to eliminate the risk of sample contamination. This makes Eppendorf LoBind Plates ideal for applications where sample recovery is key - such as the preparation of long-term storage nucleic acids in forensic, microarrays and NGS applications.
High-quality Eppendorf DNA LoBind® products
Available in a variety of formats, to help you recover nucleic acids, including tube, microplate and deepwell plate to meet the different application needs for various sample volumes and throughput.
Superior DNA recovery rate
Eppendorf DNA LoBind® Tubes recover significantly more DNA than competitor tubes. DNA (0.2 ng/µL DNA fragment (130 bp, 32P labelled) in 2.5 M NaCl/TE buffer) was incubated at various conditions before being quantified to assess sample recovery. The Eppendorf DNA LoBind Tubes revealed up to 99 % sample recovery independent of the tested incubation temperature or time.
Get consistency in quantification
Eppendorf LoBind® Tubes offer greater recovery potential when compared to a low-retention competitor. An assessment of Eppendorf LoBind tube consistency in the preparation and storage of standards for real-time PCR absolute quantifications showed that the low adsorption of DNA to LoBind tubes improved the quality of standard curves and DNA quantification in assayed samples.
Image: Preparation of DNA standards: Genomic DNA stock solution was serially diluted 10–fold in competitor low retention tubes or Eppendorf DNA LoBind® Tubes. 22 µL aliquots of each dilution were stored for 24 hours at –80 °C before analysis by real-time PCR. Samples stored in Eppendorf LoBind® Tubes had a greater PCR efficiency when compared to the competitor. See Application Note 226 for more details.
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Applications
- Preparation or storage of DNA and RNA samples
- Forensic trace analysis
- Preparing dilution series in quantitative qPCR
- Sample preparation in next-generation sequencing
- Creation of genomic or oligonucleotide libraries
Features
- Eppendorf LoBind material ensures optimized sample recovery for improved assay results
- Free of surface coating (e.g. silicone) to minimize the risk of sample interference
- Lot-certified PCR clean purity grade: free of human DNA, DNase, RNase and PCR inhibitors
- Available in tube, microplate, and deepwell plate formats for easy-up scaling
- High-contrast Unique OptiTrack® matrix: up to 30 % faster sample identification and fewer pipetting errors
- RecoverMax® well design: optimized well geometry for minimal remaining/dead volume and excellent mixing properties
- Raised well rims and a smooth surface ensure reliable sealing