How can I decontaminate and clean the Mastercycler X50 and which cleaning agents and disinfectants can I use?

Before starting cleaning or disinfection, switch off the device and disconnect it from the mains/power line. Apply cleaning agent/disinfectant to a cloth and rub the unit with this. (Note: the license sticker loses some color here). 70 % Ethanol and 70% isopropanol can be used with caution. Do not use any aggressive chemicals on the device or its accessories, such as strong and weak bases, strong acids, acetone, formaldehyde, halogenated hydrocarbons or phenol, should not be used on the device or its accessories. If the device has been contaminated by aggressive chemicals, clean it immediately using a mild cleaning agent. Be careful that no liquids penetrate the unit (please see the operating manual for more details). You can find a detailed overview of the Chemical Resistance of the Mastercycler ® X50 to further cleaning reagents and disinfectants (including DNA AWAY ® , DNA/RNA-ExitusPlus™ AWAY) on https://www.eppendorf.com, when looking for certificates of the Mastercycler X50.

What is the max sample volume that I can use in the 96-well block devices?

100 µL (for both aluminum and silver blocks)

Can I transfer data (e.g. user profiles, protocols) from my older Eppendorf thermal cyclers (Mastercycler gradient, nexus or pro families) to the new Mastercycler X50?

As the Mastercycler uses modern user interface and software structure, transfer of data from older Eppendorf Mastercyclers is not possible with the Mastercycler X50 Software version below 1.0.20.0. All Mastercycler X50 variants with Lot no. H298990L and higher will come with software V1.0.20 installed. First production lot with V1.0.20 is Mastercycler X50s (order no. 6311 000.010), starting with serial no. 6311HL208116. This new software facilitates easy transfer of PCR programs from any Mastercycler nexus or Mastercycler pro: After connecting an USB stick the programs can be imported to the Mastercycler X50.

Can the Mastercycler X50 be connected to Mastercycler pro devices in a network?

Networking is only possible within members of the Mastercycler X50 family. Up to 10 Mastercycler X50 devices can be connected in a device-controlled network while up to 50 Mastercycler X50 can be connected in a PC-controlled network.

2D-Gradient is a 2-dimensional gradient technology that allows two gradients in the same run. This allows you to optimize the annealing and the denaturation temperatures in parallel in a single run, thus saving valuable optimization time.

How can I connect a master device and an eco device of the Mastercycler X50?

One eco device can be connected to a master device directly via an Ethernet cable between the "Cycler ports". If more than one eco devices are to be connected, an Ethernet switch with 100 Mbit to 1 Gbit connection with auto-negotiation is required. Each of the master and eco devices should be connected to the Ethernet switch via Ethernet cables from the "Cycler ports".

Does the Mastercycler X50 has password protection function for users?

Yes, the Mastercycler X50 has extensive User Management functions with three level of user roles: Administrator, User with standard rights and User with restricted rights. The user accounts can be protected via Password or PIN system.

How fast is the silver block of Mastercycler X50?

The block has heating rate of 10°C/s and cooling rate of 5°C/s.

What is the temperature homogeneity of the block?

at 20-72°C: ±0.2°C at 72-95°C: ±0.3°C

Does the Mastercycler X50 has in-device self-test function?

Yes. No other accessories are required.

Where can I find license information about software components of the Mastercycler X50?

The software included in this product contains copyrighted software that is licensed under the GPL. A copy of that license is included in the settings of the device under “About this Mastercycler X50”. You may obtain the complete corresponding source code from us for a period of three years after our last shipment of this product. Please direct your request to eppendorf@eppendorf.com. This offer is valid to anyone in receipt of this information.

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Mastercycler^® X50 - PCR Thermocycler

Mastercycler® X50 PCR thermocycler - Touch screen interface

Mastercycler® X50 touchscreen and eco PCR thermocycler range, including X50h and X50r - Open units

Scientist using Mastercycler® X50 PCR thermocyclers in the lab.

Mastercycler® X50 units networked in the lab - Open units.

Cycle Manager, X50 white background Simple, Central, Smart ... Mastercycler X50 with Touchscreen

Mastercycler® X50 PCR 2D heatmap - Left to Right

Mastercycler® X50 2D heatmap - Top to Bottom

Mastercycler® X50h PCR thermocycler - Open unit, top-down view

Scientist loading samples into Mastercycler® X50 eco unit

Mastercycler® X50 PCR thermocycler in lab - Closed unit, front view showing small footprint

Mastercycler® X50h PCR thermocycler - Closed unit, top-down view

Mastercycler® X50t eco PCR thermocycler unit - Front view

Mastercycler® X50 PCR thermocycler in lab - Open unit, front view showing flexlid®

Mastercycler® X50 PCR thermocycler touch screen interface with active status display

Product Information

The new Mastercycler^® X50 elegantly combines speed, flexibility, and ease of use. With its intuitive touch screen offering precise fingertip control, the MC X50 PCR thermocycler enables easy PCR optimization in advanced molecular biology research and dependable standardization in routine PCR applications. It is also perfectly fitted for NGS.

Show more Product Information

Learn more about sustainability at Eppendorf - incl. how to recognize a sustainable PCR thermocycler

You will find additional download material at the bottom of this page

Product Information

Technical Data

Applications

Features

Available as standalone units, controlled by the user-friendly VisioNize^® touchscreen interface, or externally controlled eco units, the Mastercycler X50 family offers the flexibility to meet all your throughput needs. Mastercycler X50 eco units can only be controlled by a connected Mastercycler X50 with touchscreen, or the CycleManager X50 software.

Import your programs from slower cyclers to the Mastercycler X50 by only programming the total run time your current cycler needs. Mastercycler X50 automatically adjusts its ramp rate to the run time and the PCR will perform as you are used to. No further optimization of your proven protocols is needed.

Watch our video on how to program the desired runtime.

Each standalone PCR thermocycler can be networked with up to nine additional eco units. For even higher throughput, you can control up to fifty MC X50 eco units with our CycleManager X50 software. Whichever you need, you’ll be able to monitor and manage every unit - individually, simultaneously or as a group – with the assurance that every PCR cycler provides excellent block homogeneity, accuracy and precision. For security, simply choose different levels of access for different users. Low noise and power usage, and the versatile flexlid^® concept for maximized consumable flexibility, make the MC X50 the most powerful and convenient PCR thermocycler set-up.

Advanced PCR Optimization in a One Step

Mastercycler X50 PCR thermocyclers have a 2D-gradient function that means you can simultaneously optimize both the denaturation temperature (bottom to top) and the annealing temperature (left to right) - see the example of β-actin gene PCR optimization.

Higher denaturation temperatures can increase specificity while lower denaturation temperatures can increase yield, so with the MC X50 PCR cyclers, you can quickly determine the conditions for both optimal yield and specificity. This saves PCR optimization time, is a great benefit in low target copy number and GC-rich target applications, and is really useful in troubleshooting non-specific amplification issues.

Need rapid PCR results?

In the modern lab, fast is essential, not a luxury. PCR thermocyclers that give you quicker results throughout the day and a faster transition to optimal temperatures mean you can get through more work in a shorter time. The Mastercycler X50 PCR cycler heats at 10 °C/s and cools at 5 °C/s, so make the speed of the MC X50 your research advantage.

Simple, central, smart – The CycleManager X50

The CycleManager X50 allows control of up to fifty networked X50 cyclers. Control can be individual, simultaneous, or grouped. Perfect for high-throughput standardised protocols where reproducibility is paramount. Password protected user access is optional and users can be assigned different access rights, allowing greater documentation capabilities than ever before. It can also e-mail you when your PCR is complete!

• Individual, simultaneous or grouped control of up to fifty cyclers
• Verification / service reminders to ensure unverified cyclers are not used
• Profound documentation capabilities

Note: The CycleManager X50 can only be used with Mastercycler X50 variants without Touchscreen Interface.

Disposal of packaging

The packaging of the PCR cycler contains different materials, this includes cardboard, a plastic dust cover, and some foam parts. Recycling of materials becomes more and more important every day: Are you aware that nearly all cardboard material is recycled in Europe? Our cycler packaging cardboard material consists of approx. 60 % recycling material. Please support the global sustainability initiative of recycling valuable raw material by also collecting the cycler cardboard packaging material and disposing of it in the appropriate collection container at your organization. In respect to the plastic dust cover made of LD-PE and the foam parts, we recommend to select a dedicated recycling partner where PE material can be recycled. We suggest you contact your local waste hauler or facility management team to understand the available recycling options for your organization.

Disposal of instrument

Our cyclers last for many years, but if they need to be replaced, we kindly ask you to fulfill local requirements for disposal of these instruments. We strongly recommend a certified local recycling partner with experience in instruments with electronics. Keeping it "local" reduces the impact of transportation, and the "certified" aspect is recommended due to the safe and sustainable removal of specific parts.

Decontamination

This piece of equipment was used in a laboratory and/or was used to handle biological samples. Please keep in mind to adequately decontaminate the equipment which needs to be disposed. Check local requirements. For more information, get in contact with your local biosafety officer and/or waste officer. Check if your local recycling partner has special instructions and/or documentation requirements. You may also use the Eppendorf decontamination form sheet as guidance.

Improving ergonomics across the lab

Ergonomics goes far beyond just the »ergonomically designed chair«. At Eppendorf, we began optimizing our laboratory devices for ergonomics in the early 1970s.

In 2003, we launched the PhysioCare Concept^®, focusing on ergonomic liquid handling devices, like our pipettes.

Nowadays, the Eppendorf PhysioCare Concept has broadened to include many laboratory products, including PCR cyclers. Our aim is to offer a holistic solution for harmonizing the workflow in your laboratory with your health and well-being.

For the Eppendorf Mastercyler range, this means:
› Ergonomically designed lid closure
› Low noise level for convenient working conditions at the bench
› Small footprint to enable position in direct arm reach at your bench
› Standardized »user-interface concept« for intuitive handling of devices
› Guided software for easy handling

Further information about the Eppendorf PhysioCare Concept:
www.eppendorf.com/physiocare/

Simple, intuitive documentation and tracking

The eLabJournal^® Electronic Lab Notebook offers an intuitive and flexible solution to document research and track research data, including optimal PCR cycler temperatures and results. Improve efficiency when documenting, organizing, searching, and archiving collected data. With the free eLabJournal add-ons, you can extend the functionality of eLabJournal to a fully integrated Laboratory Information Management System.

Start a free 30-day trial!
Visit www.eLabJournal.com

Efficient lab and device management with VisioNize^® Lab Suite

Are you interested in monitoring your lab devices and receiving notifications e.g., for device alarms? A digital, connected lab offers even more.

Gain access to a modular range of digital services and choose what works for your lab. VisioNize Lab Suite offers everything you need as a Lab Manager or Scientist to get started to digitally manage your lab for increased sample security, compliance needs and maintenance management.

Chart your own course to digital lab excellence! Find out more

Read Less

Specifications	Mastercycler^® X50s	Mastercycler^® X50a	Mastercycler^® X50h	Mastercycler^® X50i	Mastercycler^® X50l	Mastercycler^® X50t

Unit can control 9 other units in network	■	■	■	–	–	–
Unit can only be controlled	–	–	–	■	■	■
Thermoblock	Silver	Aluminum	Aluminum	Silver	Aluminum	Aluminum
Sample capacity	96-well plate or 0.1/0.2 mL tubes	96-well plate or 0.1/0.2 mL tubes	384-well plate	96-well plate or 0.1/0.2 mL tubes	96-well plate or 0.1/0.2 mL tubes	384-well plate
96-well plate	■	■	–	■	■	–
384-well plate	–	–	■	–	–	■
0.1/0.2 mL tubes	■	■	–	■	■	–
Heating technology	Six peltier elements	Six peltier elements	Six peltier elements	Six peltier elements	Six peltier elements	Six peltier elements
2D-Gradient block	over 12 columns and 8 rows	over 12 columns and 8 rows	over 24 columns and 16 rows	over 12 columns and 8 rows	over 12 columns and 8 rows	over 24 columns and 16 rows
Temperature control mode	Fast, Intermediate, Standard, Safe	Fast, Intermediate, Standard, Safe	Fast, Intermediate, Standard, Safe	Fast, Intermediate, Standard, Safe	Fast, Intermediate, Standard, Safe	Fast, Intermediate, Standard, Safe
Temperature control range of the block	4 – 99 °C	4 – 99 °C	4 – 99 °C	4 – 99 °C	4 – 99 °C	4 – 99 °C
Gradient range	1 – 30 °C	1 – 30 °C	1 – 30 °C	1 – 30 °C	1 – 30 °C	1 – 30 °C
Gradient temperature control range	30 – 99 °C	30 – 99 °C	30 – 99 °C	30 – 99 °C	30 – 99 °C	30 – 99 °C
Block homogeneity 20 – 72 °C	≤±0.2 °C	≤±0.2 °C	≤±0.2 °C	≤±0.2 °C	≤±0.2 °C	≤±0.2 °C
Block homogeneity 95 °C	≤±0.3 °C	≤±0.3 °C	≤±0.3 °C	≤±0.3 °C	≤±0.3 °C	≤±0.3 °C
Control accuracy	±0.15 °C	±0.15 °C	±0.15 °C	±0.15 °C	±0.15 °C	±0.15 °C
Product type	PCR	PCR	PCR	PCR	PCR	PCR
Heating rate	max. 10 °C/s	max. 5 °C/s	max. 5 °C/s	max. 10 °C/s	max. 5 °C/s	max. 5 °C/s
Cooling rate	max. 5 °C/s	max. 2.3 °C/s	max. 2.3 °C/s	max. 5 °C/s	max. 2.3 °C/s	max. 2.3 °C/s
Lid descent	flexlid^® technology with thermal sample protection	flexlid^® technology with thermal sample protection	flexlid^® technology with thermal sample protection	flexlid^® technology with thermal sample protection	flexlid^® technology with thermal sample protection	flexlid^® technology with thermal sample protection
High pressure lid	–	–	■	–	–	■
Lid temperature range	37 – 110 °C	37 – 110 °C	37 – 110 °C	37 – 110 °C	37 – 110 °C	37 – 110 °C
Interfaces	Ethernet, USB	Ethernet, USB	Ethernet, USB	Ethernet, USB	Ethernet, USB	Ethernet, USB
Power supply	100 – 240 V, 50 – 60 Hz	100 – 240 V, 50 – 60 Hz	100 – 240 V, 50 – 60 Hz	100 – 240 V, 50 – 60 Hz	100 – 240 V, 50 – 60 Hz	100 – 240 V, 50 – 60 Hz
Max. power consumption	850 W	850 W	850 W	850 W	850 W	850 W
Total weight	11.5 kg	11.5 kg	11.5 kg	10.7 kg	10.7 kg	10.7 kg
Dimensions (W × D × H)	27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in	27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in	27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in	27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in	27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in	27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in
Remote Device Monitoring and Notifications (VisioNize^®)	VisioNize^® touch enabled	VisioNize^® touch enabled	VisioNize^® touch enabled	VisioNize^® touch enabled	VisioNize^® touch enabled	VisioNize^® touch enabled
Touchscreen Interface	yes	yes	yes	no	no	no

Eppendorf ThermoMixer^®
Heating rate	°C/s	−	−	°C/s	−	−
Cooling rate above RT	°C/s	−	−	°C/s	−	−

Downloads: Mastercycler^® X50

Flyer

Correct Temperature? – Yes!

Verify the temperature of your PCR cycler block on site

PDF .3MB

English

Customer Testimonial

Interview Henner Tasch

Mastercycler^® – tradition leads the way into the future

Simply migrate your PCR programs with one step on Eppendorf Mastercycler^® X50

PDF 1.0MB

English

Brochure

Simple, Central, Smart

The Eppendorf CycleManager X50

The new Mastercycler^® X50

Quality – Stability – Purity: Eppendorf Plates^®

PDF 4.8MB

English

Flyer

VisioNize Lab Suite Cycler Flyer

Be Confident With Every PCR Run

Increase the productivity of your NGS library preparation without compromising the reliability of your results

PDF 1.4MB

English

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Mastercycler® X50 - PCR Thermocycler